EMBEDDING AND INFILTRATION

Question 1

Process that removes the clearing agent

Answer 1

impregnation

Question 2

3 types of general tissue impregnation

Answer 2

paraffin wax impregnation
celloidin impregnation
gelatin impregnation

Question 3

range temp of actual melting wax

Answer 3

56 celcius
58 celsius

Question 4

solid in form upon procurement thus we need to melt it first in order for us to have it in
liquid form.

Answer 4

paraffin wax

Question 5

must be maintained 2-5°C above
the melting point of wax

Answer 5

paraffin oven

Question 6

what coarse filter must be used in impregnation

Answer 6

Green’s No. 904

Question 7

3 Ways of Paraffin Wax Impregnation

Answer 7

manual processing
automatic processing
vacuum embedding

Question 8

how many changes of paraffin dp we do in manual processinh and how long per beaker/change

Answer 8

3 changes of paraffin, 1hr per beaker/change

Question 9

how many stations are there in automatic processing and infiltration will take up at what stations?

Answer 9

12 station; 11 and 12

Question 10

fastest way of embedding yet the most expensive

Answer 10

vacuum embedding

Question 11

more elastic and resilient

Answer 11

Paraplast

Question 12

Less brittle, and less compressible

Answer 12

Embeddol

Question 13

semisynthetic; for embedding of eyes

Answer 13

Bioloid

Question 14

melting point of paraplast

Answer 14

56-57°C

Question 15

melting point of embeddol

Answer 15

56-57°C

Question 16

melting point of ester wax

Answer 16

46-48°C

Question 17

melting point of water soluble waxes (polyethylene glycol)

Answer 17

38-42 or 45-56 celsius

Question 18

most common water soluble wac and no need for dehydration and clearing

Answer 18

Carbowax

Question 19

remedy for carbowax

Answer 19

add soap to water or 10% PEG 900 in water

Question 20

Purified form of nitrocellulose/gun cotton

Answer 20

colloidin

Question 21

concentrations in celloidin impregnation

Answer 21

2%
4%
8%

Question 22

• for bones, brain, teeth
  ◆ Store tissue block in 70%-80% alcohol
  ◆ The purpose of storing it in this
  concentration is to avoid dehydration and
  shrinkage of the tissue.

Answer 22

wet

Question 23

• for whole eye sections
  ◆ Store tissue block in Gilson’s Mixture
  (chloroform and cedarwood oil)

Answer 23

dry

Question 24

Has lower viscosity, thus can be used in higher concentration, and rapid tissue penetration

Answer 24

Nitrocellulose

Question 25

Needed to prevent tissue cracking in chrome mordanted tissues

Answer 25

Plasticizer

Question 26

rarely used impregnation

Answer 26

gelatin impregnation

Question 27

must be added to prevent molds and prevent further damage such as tissue decay

Answer 27

1% phenol

Question 28

Placing the impregnated tissue into a mold with embedding media, and then allowing the media to solidify

Answer 28

embedding

Question 29

other terms for embedding

Answer 29

casting blocking molding

Question 30

orientation for tubular tissue

Answer 30

transverse sections

Question 31

orientation for skin

Answer 31

all layers should come

Question 32

orientation for endomatrial curetting

Answer 32

keep in center

Question 33

orientation for long tissue

Answer 33

keep diagonally

Question 34

orientation for intestine

Answer 34

all layers should come

Question 35

orientation for membrane

Answer 35

swiss roll

Question 36

types of molds

Answer 36

Leuckhart’s Embedding Mold Compound Embedding Unit Plastic Embedding Rings and Base Mold Disposable Embeding Molds

Question 37

It has two (2) L-shaped metal plates, strips of heavy brass metal.

Answer 37

Leuckhart’s Embedding Mold

Question 38

This used for batch embedding thus one of its disadvantages is being prone to the interchanging of samples.

Answer 38

Compound Embedding Unit

Question 39

This is for the ease of separation of the tissue block from the mold after it solidifies.

Answer 39

Disposable Embeding Molds

Question 40

Tissues are first infiltrated with Celloidin and subsequently embedded in a paraffin mass; For large blocks of dense tissues; obsolete

Answer 40

double-embedding method

Question 41

For high resolution light microscopy of thinner than usual sections, renal biopsies, BM biopsies

Answer 41

plastic resin embedding

Question 42

Most widely applied, but carcinogenic due to vinylcyclohexane dioxide (VCD) component

Answer 42

epoxy

Question 43

types of epoxy

Answer 43

● Bisphenol A (Araldite) - slow ● Glycerol (Epon) - low viscosity ● Cyclohexene dioxide (Spurr) - very low viscosity; fastest

Question 44

For electron microscopy; seldom used

Answer 44

polyster

Question 45

For high resolution light microscopy

Answer 45

acrylic plastics

Question 46

catalyst; forms radicals which are site for polymerization

Answer 46

benzoyl peroxide