Fish, amphibians and reptiles in the lab Flashcards Preview

Laboratory Animal Science > Fish, amphibians and reptiles in the lab > Flashcards

Flashcards in Fish, amphibians and reptiles in the lab Deck (9):
1

Why is the zebra fish a good model?

  • small, can be kept in large numbers
  • females can spawn every 2-3 days
  • short generation time, 3-4 months
  • sequenced genome
  • increasing interest in behaviour

2

Why is the stickleback a good model?

  • well studied species
  • very popular in behavioural studies
  • becoming popular in tox studies
  • genome sequenced

3

Pain in fish

 

  • rainbow trout has the same type of nociceptors as mammals
  • as in mammals pain information from head and mouth conveyed by the trigeminal nerve
  • respond behaviorally to noxious stimuli
  • this response is reduced by analgesic (morphine)

4

Name some stressors for fish

  • hypoxia
  • bad water quality
  • temperature
  • handling
  • transport crowding
  • social interactions

5

Physiological effects of sympathetic activation and elevated plasma catecholamine concentrations

  • increased HR
  • stimulation of breathing, oxygen uptake and transport
  • increase in blood flow to gills, brain and skeletal muscles
  • reduction in blood supply to the GI tract
  • elevelated blood glucose levels

6

Why is it costly to breath water?

  • holds lower oxygen concentration than air
  • has a higher density than air
  • is more viscous than air
  • oxygen has a lower diffusion coefficient in water than in air

7

Effects of air exposure in fish

  • drastically reduced O2 levels in the blood
  • increase in blood pCO2
  • larger increase in blood and muscle lactate concentrations
  • larger decrease in blood and muscle pH
  • even a very short air exposure results in drastically elevated mortality
  • gills collaps in gravity

8

How to reduce the effects of stress in fish

 

  • low temperature
  • starve the fish
  • NaCl to the water

9

Behavioural effects of social subordination

  • suppression of aggressive behaviour
  • decreased appetite and food intake
  • lowered locomotor activity
  • a general inhibition of behavioural responsiveness