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Flashcards in Genetic standardisation Deck (9):
1

What are outbred lines also called?

stocks

 

2

What are inbred lines also called?

strains

3

Name some characteristics of outbred stocks?

  • heterozygosity undefined
  • genetic profile unkown
  • random-mated, close colonies
  • high reproductive performance and high vigor
  • phenotypically variable
  • not identifiable - not defined by genetic profile
  • cheap and large

resemble human population

4

How are inbred strains defined?

The result of more than 20 consecutive generations of brother-sister mating

An inbred strain may be regarded as an immortal clone of an almost constant genotype

5

Name some properties of inbred strains

  • isogenicity
  • homozygosity
  • phenotypic uniformity
  • long term stability
  • individuality of the strain
  • internatl distribution
  • identificaition (PCR primers available)
  • senstivity (to, e.g., environmental factors)

6

When should outbred stocks be used in research?

  • large amounts of tissue
  • education
  • base population for selective breeding
  • genetic mapping

7

When should inbred strains be used?

In all cases where use of outbred stocks is not particularly justified

8

What are F1 hybrids?

  • Cross between 2 inbred strains
  • heterozygosity high
  • isogenic
  • robust - high vigour

9

Name main causes for genetic change

  • genetic contamination or immigration
  • mutation
  • directional selection
  • random drift