Histology - Intro to Histology Flashcards Preview

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Flashcards in Histology - Intro to Histology Deck (19)
1

, focused with optincal lens (max resoving poswr ~0.2 microns).

Light microscopy (LM)

2

Electrons interact with tissue components (B&W images). Focused with electromagnetic lens (resolution 1000x LM)

Electron microscopy (EM)

3

Shows internal structure

Transmission (TEM)

4

Shows external (surface) structure

Scanning (SEM)

5

splits cell membranes (p-face, e-face)

TEM freeze fracture technique

6

Interact with acids (basophilic structures). Stain acidic components blue or purple. E.g. nucleic acids (RNA< DNA, RER), glycosaminoclycans (GAGs)

Base-like stains

7

Interact with bases (acidophilic structures). Stain basic components pink, red, or orange. E.g. mitochondria, collagen

Acid-like stains

8

acid-like stain (reddish)

eosin

9

base-like stain (blue)

hematoxylin

10

Stains carbohydrates magenta. E.g., glycoproteins in mucin

Periodic-Acid Schiff (PAS)

11

coffee bean staining pattern of nucleus

Brenner tumor

12

Stains collagen fibers blue or green

Trichrome

13

Stains reticular fibers black

Silver

14

Stains elastic fibers reddish-brown or black

Orcein

15

stains lipids red

Sudan Red

16

stains lipids black

Osmic acid

17

Look at nuclei (tend to be similar in size among cells)

How to see scale

18

biological yardstick (~8 microns)

Red blood cell (RBS)

19

tissue shrinkage & lost molecules, “cracks” & “spaces”

Artifacts