HPV and HPolyV Flashcards Preview

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Flashcards in HPV and HPolyV Deck (19):
1

structure of Human polyoma virus and Human papilloma virus

naked icosahedral
circular dsDNA

2

human polyomaviruses are usually seen in....

immunocompromised:
organ transplantation/HIV infection

3

route of human polyoma virus transmission

enters through respiratory tract; can get into blood and remain latent until reactivated by immunocompromisation

4

reactivation of JC virus can cause....

reactivation is most common in...

progressive multifocal leukoencephalopathy(PML)
- transforms astrocytes
- kills oligodendrocytes
- demyelinating lesions

common in CD4<50; AIDS pts

5

reactivation of BK virus can cause....

reactivation is most common in...

polyomavirus-associated nephropathy after solid organ transplant

hemorrhagic cystitis after stem cell transplant

6

HPV capsid proteins

L1, L2

7

genes of HPV genome

early: E1-8
late: L1,L2
long control region(LCR)

8

transmission of HPV

vaginal/anal/oral sex
mother>>newborn

9

pathogenesis of HPV

virus gets into basal layer of stratified epithelium via micro-tears

L2-DNA complex moves into nucleus as episome

E6/E7 induce cell proliferation by inhibiting p53/Rb

Viruses are assembled by the time cells reach cornosum layer and are shed at surface

10

how does carcinogenesis occur in HPV?

integration into host genome can cause loss of E2 which regulates E6/E7 expression

if E2 is lost, E6/E7 will constitutively inhibit p53/Rb and cause tumor formation

11

HPV 6,11 primarily cause

mucosal warts/cutaneous infection

recurrent respiratory papillomatosis

12

HPV mucosal warts can progress to....

low grade CIN/AIN(cutaneous/anal neoplasias)

regress in most adults w/in 5 years

13

manifestation of HPV involving recurrent laryngeal tumors, hoarseness, stridor, respiratory distress
can be child onset from birth canal

recurrent respiratory papillomatosis

14

most common HPV types causing cancer

HPV 16 and 18
more likely a resistant strain if same HPV is detecte 2x in 6-12 mo.

15

high risk HPV types

16,18,31,33,45,62,58

16

low risk HPV types

6,11

17

diagnosis of HPV

molecular detection of RNA/DNA is gold standard

18

treatment for HPV

surgical removal of lesions but no treatment for HPV

19

HPV vaccines; types and mechanism

L1 protein is recombinantly generated in yeast, which spontaneously assembles into an empty icosahedral shell

Gardasil(6,11,16,18) - male/female
cervaris(16,18) - females