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Instrumentation Flashcards

1
Q

Most common Glassware

A

Borosilicate Glass

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2
Q

Temperature Rate of Borosilicate Glass

A

515 C

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3
Q

Borosilicate Glass has high ____________ resistance, but low __________ resistance.

A

High Thermal Resistance
Low Alkali Resistance

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4
Q

Boron-free Glass

A

a.k.a Soft Glass
High Alkali Resistance

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5
Q

What contains Alkali Resistant Glass?

A

Magnesium Lime

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6
Q

Pyrex, Kimax

A

Borosilicate Glass

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7
Q

Specialized alumina Silicate Glass

A

Coring

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8
Q

Corex is ______ times stronger than borosilicate glass

A

Six (6)

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9
Q

Temperature rate of Vycor

A

900 C

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10
Q

Vycor

A

High thermal drastic-heat shock resistance

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11
Q

Vycor is both _______ and _______ resistant

A

Acid and Alkali

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12
Q

Corex is strengthen ___________ than __________.

A

Strengthen CHEMICALLY than Thermally

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13
Q

It is used for Disposable glasswares

A

Flint Glass/Soda Lime Glass

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14
Q

Most Common and Simplest type of Pipette

A

Glass Pipet

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15
Q

Enumerate Classification of Pipette

A

According to Calibration
1. To Contain
2, To Deliver

According to Drainage Characteristic
1. Blow Out
2. Self-draining

According to Purpose
1. Transfer Pipets
- Volumetric
- Ostwald-folin
- Pasteur
- Automatic micropippete
2. Measuring/Graduated Pipets
- Serologic
- Mohr
- bacteriologic
- Ball, Kolmer, Kahn Pipets
- Micropipettes

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16
Q

Transfer pipet that is used for NON-VISCOUS fluid and is SELF DRAINING

A

Volumetric Pipet

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17
Q

Ostwald Folin

A

Viscous fluid and Blow out

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18
Q

Pasteur pipet

A

Transfer fluid without consideration of specific volume

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19
Q

Automatic Micropipette

A

Most sensitive

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20
Q

Serologic pipet has ____________ to the tip and is ___________.

A

Graduation to the tip
Blow Out

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21
Q

Mohr pipet has ___________ to the tip and is ____________.

A

No graduation to the tip
Self-draining

22
Q

Recall: An automated pipet that rely on PISTON FOR SUCTION and DISPOSABLE TIPS.

A

Air Displacement Pipette

23
Q

Recall: An automated pipet that rely by MOVING THE PISTON in the pipet tip and has PERMANENT TIP.

A

Positive Displacement Pipet

24
Q

Micropipette Tips are made from ____________.

A

Polypropylene

25
Cleaning solution for Glasswares
Acid Dichromate
26
Recall: Polypropylene
Autoclaved Common Micropipette tips/Plastic Pipette tips
27
Used for Plastic test tube and Test Tube Rack
Polycarbonate
28
4 Basic Approaches to Automation
Continuous Flow Centrifugal Analyzer Discrete Analyzer Dry Slide
29
Continuous Flow Analyzer
Reagents, diluents and samples are pumped through a system of CONTINUOUS TUBING Mixing: Glass Coil
30
Uses Batch Testing Analysis
Centrifugal analyzer
31
Centrifugal Analyzer
One test per sample but loaded at the same time. Mixing: Spinning Rotor (1000 rpm)
32
Used for STAT
Discrete Analyzer
33
Discrete Analyzer
Measures only the test requested on a sample and each sample is analyzed is separately in their own reaction container. Mixing: Magnetic stirring bar
34
Batch Testsing
All sample are loaded at the same but one single test is conducted per sample.
35
Parallel Testing
Simultaneous analysis
36
Random Access Tesing
Any sample, any test, any sequence
37
Sequential Testing
Multiple test are analyzed one after another each specimen
38
Open Reagent System
Can use other than manufacturer's reagent
39
Close Reagent System
Only the manufacturer's reagent can be used
40
Time of Pipette Calibration in a year
3-4 times/year
41
Class ___ pipet do not require Calibrations.
Class A
42
QC: Used to verify pipette volume and accuracy
Gravimetric and Spectrometric Method
43
Calibrating medium - TD Pipette
Distilled H2O
44
Calibrating medium - TC Pipette
Mercury
45
Used to compare reproducibility of Pipet tips brands
0.1% Phenol Red solution in distilled H2O
46
Serves as separation and cleaning in Continuous Flow analyzers
air bubbles
47
Rapid and Ideal Centrifuge
Fixed Angle Head
48
Horizontal Head Spinning: Not spinning:
Horizontal Head Spinning: Horizontal Not spinning: Vertical
49
Type of Reagent Water Type I: Type II: Type III:
Type of Reagent Water Type I: Standard solution Type II: Reagent Preparation Type III: Washing
50
Types of water used for consumption and cooking ? a. distilled b. potable c. purified d. AOTA
B. Potable
51
Most popular and versatile analyzer
Discrete Analyzer
52
Minimum volume of Discrete Analyzer
2-6 ul

Clinical Chemistry (20 decks)

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