Specimen Preparation, Handling, Transport & Storage

Question 1

Preanalytical Variable in Px. Preparation

Answer 1

Age & Gender
Diurnal Variation
Posture
Fasting
Diet
Drugs
Stress

Question 2

Exercise - Transient Increase

Answer 2

Lactate, FA, Ammonia

Question 3

Fasting Specimens

Answer 3

FBS
GTT
Lipid Profile
Gastrin
Insulin
Aldoterone/Renin

Question 4

Over fasting - 72 hrs

Answer 4

Increased in Males: TAG
Decreased in Females: 45mg/dl of Glucose

Question 5

Increased during standing

Answer 5

Albumin
Cholesterol
Aldosterone
Calcium

Question 6

Increased - From Supine to Sitting

Answer 6

Albumin
Enzymes
Calcium

Question 7

Increased - From Sitting to Supine

Answer 7

Proteins
Lipids
BUN
Iron
Calcium

Question 8

Increased - Prolonged tourniquet application

Answer 8

Potassium
Proteins
Enzymes
Lactate
Cholesterol
Ammonia

Question 9

Increased - Fist Clenching

Answer 9

Potassium
Phosphate
Lactate

Question 10

Analytes Increased in AM

Answer 10

ACTH
Iron
Cortisol

Question 11

Analytes Increased in PM

Answer 11

GH
ACP

Question 12

Calcium is ___& decrease supine

Answer 12

4%

Question 13

Iron decreases up to ___% during the day.

Answer 13

30%

Question 14

Decreased cortisol level by 8:00 pm

Answer 14

50%

Question 15

Uric acid levels peak in men in their

Answer 15

20s

Question 16

Increase of Cholesterol/TAG per year

Answer 16

TG - 2mg/dL (0.02 mmol/L)
TC - 2 md/dL (0.05 mg/dL)

Question 17

Sleeping patients

Answer 17

Should be awakened

Question 18

Outpatient/Ambulatory Patient is counterchecked with:

Answer 18

Driver’s License or Valid ID with photo

Question 19

3 ways of ID

Answer 19

Positive ID: Verbal ID statement
Passive ID: Check ID band
Comparison of labeled specimen with px. ID before leaving

Question 20

Average human blood

Answer 20

5 quartz (4.73 L)

Question 21

Venous Blood

Answer 21

Deoxygenated, Dark Red Color

Question 22

Most Common Site for Venipuncture

Answer 22

Antecubital fossa - (1) Median (2) Cephalic (3) Basilic

Question 23

CLSI Recommended site for Venipuncture

Answer 23

Median Cubital Vein

Question 24

international Standard - A backflow is first seen in the ______________

Answer 24

Flash window of the Needle hub

Note: If no flash window in the choices, answer - needle hub

Question 25

Most common Antiseptic

Answer 25

70% Alcohol

Question 26

Used for Ethanol Testing

Answer 26

Benzalkonium chloride/Zephiran Chloride (1:750)

Question 27

CLSI recommended - blood culture, Infants, Iodine Sensitivity

Answer 27

Chlorhexidine gluconate

Question 28

Tourniquet Application

Answer 28

3-4 (7.5-10 cm) inches ABOVE the site for <1 minute

Question 29

CLSI - Tourniquet Reapplication

Answer 29

2 minutes

Question 30

Bacteria transmitted due to Reusable tourniquets

Answer 30

MRSA - Methicillin-resistant Staphylococcus aureus

Question 31

10% contamination of 5% glucose increase glucose by _____ mg/dL

Answer 31

500 mg/dL

Question 32

Needle Angle (Veni)

Answer 32

<30 degrees 15-30 degree

Question 33

Needle - Yellow Cap gauge

Answer 33

20 gauge

Question 34

Needle - Green Cap Gauge

Answer 34

21 gauge

Question 35

Needle - Black Cap Gauge

Answer 35

22 gauge

Question 36

Standard gauge for Venipuncture

Answer 36

21 gauge

Question 37

Pediatric/Small veins needle gauge

Answer 37

23 gauge

Question 38

Blood Letting Gauge

Answer 38

16-G

Question 39

Lumbar puncture needle gauge

Answer 39

18-G

Question 40

25-G

Answer 40

Infants/Neonates/Blood from Scalp needle Gauge

Question 41

Routine Needle Length

Answer 41

1-1.5 inches

Question 42

Butterfly Needle Length

Answer 42

1/2 - 3/4 (0.5-0.75) inch

Question 43

Cause of Hemolysis

Answer 43

Prolong tourniquet application Alcohol contamination Small needle bore Agitation Frothing/Bubbling

Question 44

Lancet Length Disposable: ________ Non-disposable: ______ Rodriguez - _____ NCBI - _____

Answer 44

Lancet Length Disposable: 0.75 mm Non-disposable: 1.75 mm (Rodriguez) 2.8 - 3.2 mm (NCBI)

Question 45

Most Routine Site for Capillary Puncture

Answer 45

4th Finger

Question 46

Sites to avoid in Capillary Puncture

Answer 46

Thumb 5th Finger

Question 47

Depth of Puncture Children/Adults

Answer 47

<2.0/2-2.5 mm

Question 48

Detected analytes in Arterialized Capillary Blood

Answer 48

pH pCO2

Question 49

Preferred site for Arterialized Capillary Blood

Answer 49

Earlobe

Question 50

First microcollection tube to be filled in Skin puncture

Answer 50

EDTA/Lavander

Question 51

Order of Draw - Skin Puncture

Answer 51

(B)lood gases (S)lides (ED)TA (O)ther microcollection tubes (S)erum container