Lab 6

Question 0

What does a negative stained bacteria look like

Answer 0

Bacteria appears clear against Gray background

Question 1

Define negative stain

Answer 1

Stains the background

Question 2

What is considered a colloidal stain

Answer 2

India ink or eosin

Question 3

What stain did we use

Answer 3

Nigrosin

Question 4

Do you heat fix in a negative stain ? And why

Answer 4

No. Less distortion so it shows morphology and size

Question 5

What are cocci bacilli

Answer 5

Short, oval bacilli

Question 6

What bacteria did we use in this lab

Answer 6

B. Subtilus

S. Epidermidis

Question 7

Describe the procedure of a negative stain

Answer 7

Please drop of Nickerson near one end of slide. Mix new full of broth culture in drop. Draw slide across surface like blood sample. Let smear air dry.