Lab microbes and mediums to know Flashcards

(37 cards)

1
Q

What bacteria type is E. coli

A

gram negative cell wall

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2
Q

What shape is E. coli

A

bacillus shaped

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3
Q

Does E. coli fermentate

A

yes it fermentated glucose and lactose

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4
Q

Is E. coli motile?

A

yes

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5
Q

What bacteria type is P. fluorescens

A

gram negative cell wall

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6
Q

What shape is P. fluorescens

A

bacillus shaped

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7
Q

Does P. fluorescens fermentate

A

no

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8
Q

Does P. fluorescens have the ability to be motile

A

yes flagellum for mobility

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9
Q

Where was their growth in the test tube for P. fluorescens, what type of oxygen relationship

A

Growth at the top; obligate aerobe

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10
Q

What type of oxygen relationship is E. Coli

A

facultative anaerobic bacterium

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11
Q

What type of bacteria is B. cereus

A

Gram positive

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12
Q

What shape is B. cereus

A

bacillus shaped

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13
Q

Is B. cereus motile

A

yes

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14
Q

What type of oxygen relationship does B. cereus have

A

aerobic or facultatively anaerobic

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15
Q

Does B. cereus fermented lactose and glucose

A

yes

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16
Q

Is S. Cerevisiae a bacteria

A

no–chitin cell wall

17
Q

What type of shape is S. Cerevisiae

18
Q

What type of growth was seen in the thioglycolate of E. faecalis; what’s its oxygen relationship

A

growth throughout; facultative anaerobe

19
Q

What was seen in the thioglycolate of S. marcescens; what’s its oxygen relationship

A

Cloudy throughout with dark red at the top; facultatively anaerobe

20
Q

What type of bacteria is S. epidermidis

A

gram positive

21
Q

What shape is S. epidermidis

22
Q

Is S. epidermidis + for glucose fermentation

23
Q

Is S. epidermidis motile

24
Q

What type of medium is nutrient broth/NA

A

all purpose medium

25
What type of medium is MAC
selective and differential medium (for gram negative bacteria)
26
What will MAC look like if fermentation occurs after 24 hours of incubation
red colonies
27
What type of medium is APT w/ tween
non selective (typically fastidious bacteria)
28
What are the differential ingredients in MAC
lactose neutral red
29
What are the selective ingredients in MAC
bile salts Crystal violet
30
What is the selective ingredient in APT w/ tween
sodium azide
31
What can thioglycolate help determine
obligate anaerobes
32
What type of medium is thioglycolate
differentail
33
What type of medium is fermenting broth
differential medium for fermentation
34
What color will carbone fermentation broth turn if fermentation has occured
yellow due to the bromcresol purple
35
What type of medium is EMB
selective and differential
36
What is the selective ingredient for EMB
Esoin Y and Methylene blue dyes
37
What is the differential ingredient for EMB
lactose