Chapter 3 notes Flashcards

(47 cards)

1
Q

Define infection

A

a microbe that invades a human or other organism and causes illness and disease

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2
Q

What two things do growth of microbes include

A

–catabolic and anabolic process to increase cell size
–make more copies of itself

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3
Q

How do eukaryotes reproduce

A

sexually AKA meiosis

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4
Q

How do prokaryotes reproduce

A

binary fission

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5
Q

What does the parent cell of binary fission have

A

ribosomes and chromosomes

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6
Q

What are the steps of binary fission

A
  1. parent cell
  2. duplicating chromosomal DNA
  3. chromosomes separate and attach to cytoskeleton
  4. septum divides the cell in half
  5. two daughter cells
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7
Q

Out of the 2 daughter cells, which cell is the parent cell and which cell is the daughter cell

A

they’re both new parent

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8
Q

Equation for generation time

A

G = t/n

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9
Q

Define generation time

A

time per complete fission cycle of the population or doubling of population

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10
Q

growth can cause what with max rate of population

A

maxes out rate of binary fission

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11
Q

What is the equation for predicting population size over time

A

Nf = (Ni)2^n

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12
Q

What is the blood culture test

A

mix it with a medium, put sample in media to check for growth

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13
Q

Define incubated

A

grown under optimal conditions

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14
Q

Why do we streak plates

A

to isolate individual cells/colonies

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15
Q

CFU is the number of

A

visible colonies formed

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16
Q

What are the characteristics of H. infleunzae

A

gram negative
non motile coccobacillary
facultatively anaerobic

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17
Q

Is there blood agar growth in H. influenza

A

NO–BAP lack specific nutrients required for growth of H. influenza

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18
Q

What are the characteristics of S. Aureus

A

gram positive cocci
non motile
facultative anaerobe

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19
Q

is there growth in blood agar of S. aureus

20
Q

Characteristics of M tuberculosis

A

gram positive rod shaped
strict aerobic, acid fast

21
Q

is there growth in blood agar of M. tuberculosis

22
Q

Characteristics of S. pneumoniae

A

gram positive
coccus
aerotolerant anaerobe

23
Q

Is there blood agar growth in S. pneumoniae

24
Q

Define blood agar

A

rich medium containing 5% sheep blood that can differentiate bacteria based on their hemolytic properties

25
Is blood agar complex media
yes
26
What are the effects on RBC of alpha hemolysis
partial destruction
27
What does alpha hemolysis look like on a plate
light colored green around colonies
28
What is the effect of beta hemolysis on RBC
total destruction
29
What will the plate look like with betal hemolysis
clear zones around colonies
30
What are the effects of gamma hemolysis to RBC
no damage
31
What does the plate look like with gamma hemolysis
no change on plate
32
What do we measure to measure growth over time
turbidity
33
What is the most accurate way to measure grwoth rate
viable plate counting
34
The real image is projected to the _______ where it is magnified again to form the ______ image
real; virtual
35
What are the visible wavelength of light
400nm to 750nm
36
Define total magnification
product of the separate magnifying powers of the two lenses
37
Electron waves are 100,000 times _______ than the waves of visible light
shorter
38
What is the magnification for light micro
between 40x and 2000x
39
What is the magnification for light micro
between 5,000x and 1,000,000x
40
In electron microscopes the image is due to
electron scattering
41
In light microscopes the image formation depends upon
light absorption in the object
42
Define simple stains
use one dye to stick to cells and give them color
43
Define negative stains
dye doesn't color specimens but dries around its boundary forming a silhoutte
44
In spore stains what color is the spore and cell
spore green cell red
45
What are the 3 differential stains
gram spore direct fluorescent
46
What is a structural stain
special stain to enhance details
47
What are the 2 structural stains
capsule stain flagellar stain