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Anatomical Sciences 1 > Lecture 3- Histology > Flashcards

Lecture 3- Histology Flashcards

1
Q

What can you see with low to intermediate magnification?

A

No individual cells

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2
Q

What can you see with high magnification

A

Cellular/ nuclear detail

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3
Q

What kind of magnification is this

A

Low

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4
Q

What kind of magnification is this

A

Intermediate

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5
Q

What kind of magnification is this

A
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6
Q

What is the diameter of a RBC

A

8 μm

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7
Q

What are the 3 types of microscopic imagery

A

Light microscopy, scanning electron microscopy, transmission electron microscopy

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8
Q

What does light microscopy use? It highlights what?

A

Photons, gross structure

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9
Q

What does scanning electron microscopy highlight?

A

Surface structure

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10
Q

What does Transmission electron microscopy highlight?

A

Internal cell structure

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11
Q

What produces this kind of image?

A

Light microscopy

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12
Q

What produces this kind of image?

A

Scanning electron microscopy

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13
Q

What produces this kind of image?

A

Transmission electron microscopy

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14
Q

What has the lowest resolution of microscopic imagery?

A

Light microscopy

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15
Q

With TEM what is a black structure

A

Electron dense

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16
Q

H&E stain relies on what

A

Electrostatic linkage between dyes and tissues

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17
Q

What is the most common stain

A

H&E

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18
Q

What stains dark blue/purple with H&E?

A

Components with a net negative charge (DNA/RNA/ glycosaminoglycans)are attracted to hematoxylin. Called Basophilic & stain blue

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19
Q

What stains pink with H&E stain

A

Components with a net positive charge (actin, myosin, collagen) attract Eosin. These are acidophilic and stain pink.

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20
Q

What is a limitation of H&E stain

A

Differentiating between collagen & muscle

21
Q

What is the specialized collagen stain

A

Trichrome

22
Q

What is Trichrome used to distinguish between

A

Collagen from muscle & epithelium

23
Q

What is Trichrome used to diagnose

A

Scar tissue (liver cirrhosis) & smooth muscle tumors

24
Q

With Trichrome stain what color does collagen stain? Muscle & epithelium? RBC? Nuclei?

A

Collagen: blue or green
Muscle & epithelium: red
Red blood cell: orange/red
Nuclei: brown/black

25
Q

What test is ordered to look for carbohydrate rich areas?

A

Periodic acid- Schiff (PAS)

26
Q

What is PAS used to diagnose

A

Mucus secreting tumors & basement membrane disorders

27
Q

With a PAS stain what color do carbohydrate rich areas stain

A

Magenta

28
Q

What test is ordered to diagnose fat tumors and fatty liver disease

A

Sudan red or osmic acid (black)

29
Q

Sudan red or osmic acid (black) is used to highlight lipids in what cell types

A

Adipocytes & hepatocytes

30
Q

What is a silver stain looking for

A

Damage to reticular fibers

31
Q

Silver stain is used to diagnose damage to what

A

Filtration organs and basement membranes

32
Q

A reticular fiber appears ______ with a silver stain

A

Branchy

33
Q

Elastic fibers appear ____ with an orcein stain

A

Wavy

34
Q

What is an Orcein stain looking for

A

Damage to elastic fibers

35
Q

What can an Orcein stain diagnose

A

Inflammatory diseases of dermis and arteries that damage elastic fibers

36
Q

Chemical stains highlight what

A

Classes of molecules but not a specific molecule

37
Q

What does immunofluorescent staining do

A

Uses an antibody to tag a specific molecular target, then a dye is linked to the antibody

38
Q

What kind of stain is this

A

Immunofluorescence

39
Q

What produced this image?

A

Light microscopy

40
Q

What produced this image?

A

Scanning electron microscopy

41
Q

What produced this image?

A

Transmission electron microscopy

42
Q

General base-like stains highlight what

A

Things with a net negative charge are called basophilic substances & they stain blue/ purple

43
Q

General acid-like stains highlight what

A

Components with a net- positive charge which are called acidophilic substances & stain pink

44
Q

What kind of stain made this image?

A

H&E

45
Q

What kind of stain made this image?

A

Trichrome

46
Q

What kind of stain made this image?

A

PAS

47
Q

What kind of stain made this image?

A

Sudan red or osmic acid

48
Q

What kind of stain made this image?

A

Silver

49
Q

What kind of stain made this image?

A

Orcein

Anatomical Sciences 1 (20 decks)

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