MicroLab Quizzes

Question 0

What happens to the light as you move to more powerful objectives?

Answer 0

Gets dimmer

Question 1

What does it mean if objectives are parfocal?

Answer 1

Partially focused

Question 2

What happens to field of view as you move to more powerful objectives?

Answer 2

Gets smaller

Question 3

What is the only thing you should use to clean the ocular or objective lenses?

Answer 3

Lens paper

Question 4

Objective band color when the objective is on scan?

Answer 4

Red

Question 5

When the objective color band is yellow what is the objective on?

Answer 5

Low power

Question 6

Objective band color when the objective is on high power?

Answer 6

Blue

Question 7

Objective band color when the objective is on oil immersion?

Answer 7

White

Question 8

What is the ocular magnification when the objective is on low power?

Answer 8

10x

Question 9

What is the ocular magnification when the objective is on oil immersion?

Answer 9

10x

Question 10

What is the objective magnification when the objective is on scan?

Answer 10

4x

Question 11

What is the objective magnification when the objective is on low power?

Answer 11

10x

Question 12

Which objective is at 400x total magnification?

Answer 12

High power

Question 13

What happens when the objectives you see move to more powerful objectives?

Answer 13

Gets larger

Question 14

Which objective is at 1000x total magnification?

Answer 14

Oil immersion

Question 15

What is used to clean the microscope lenses?

Answer 15

lens paper

Question 16

If you saw something a furry colony, what type of organism is it?

Answer 16

mold

Question 17

True or False: normally petris dishes are placed in the incubator upside down.

Answer 17

true

Question 18

Where are petri dishes suppose to be labeled?

Answer 18

on the bottom where the agar is

Question 19

true or false: normal hand washing removes the bacteria from your skin, so nothing grew on the petri dish

Answer 19

false

Question 20

What temperature is the incubator set as in degrees Celcius?

Answer 20

37*

Question 21

How much inoculum (bacteria) do you use when making a smear from a broth culture?

Answer 21

6-8 loopfuls

Question 22

How much inoculum (bacteria) do you use when getting it from a slant?

Answer 22

pinpoint

Question 23

List 2 reasons why you fix a smear.

Answer 23

• to keep the bacteria & stain in place
• Shrink the cell
• kill bacteria

Question 24

List an example of a basic stain

Answer 24

methylene blue

Question 25

What kind of bond forms between the basic stain and the bacteria?

Answer 25

ionic bond

Question 26

Give an example of an acidic stain.

Answer 26

congo red

Question 27

What does an acidic stain do?

Answer 27

stain the background

Question 28

Give an example of a basic stain?

Answer 28

maneval stain

Question 29

What does a basic stain do?

Answer 29

stain the cell

Question 30

How did the capsule look after the capsule stain procedure?

Answer 30

clear/white

Question 31

Why do you never heat fix a capsule stain?

Answer 31

it shrinks the cell

Question 32

What did we use in the capsule stain procedure to make the bacteria stick to the slide?

Answer 32

sheep serum

Question 33

List 2 bacteria we used for Gram staining, designate their Gram (+/-) and their final color

Answer 33

Escherichia coli: Gram (-): pink | Staphylococcus aureus: Gram (+): purple

Question 34

What is the counter stain in the Gram stain procedure?

Answer 34

Safranin

Question 35

What is the primary stain in the Gram stain procedure?

Answer 35

Crystal violet stain

Question 36

What is the primary stain in the spore stain?

Answer 36

malachite green

Question 37

What is the counter stain in the spore stain?

Answer 37

safranin

Question 38

In the spore stain, the spore appears as

Answer 38

green ovals

Question 39

What color are acid-fast bacteria after staining?

Answer 39

reddish-purple

Question 40

What is the primary stain in the acid-fast stain?

Answer 40

carbol fuschin

Question 41

What is the counter stain in teh acid-fast stain?

Answer 41

methylene blue

Question 42

List a species of bacteria that would be spore-forming.

Answer 42

Bacillus subtilis,

Question 43

List a disease cased by a spore-forming bacterium

Answer 43

botulism

Question 44

List a species of bacteria that would be acid-fast.

Answer 44

Mycobacterium smegmatis

Question 45

List a disease caused by an acid-fast bacterium

Answer 45

tuberculosis (TB)

Question 46

What procedure could we perform that would separate mixture of different kinds of bacteria?

Answer 46

streak plate isolation

Question 47

What does a colony start out as?

Answer 47

1 bacterium that divides multiple times and isolates itself from other bacteria

Question 48

What are 2 problems with cultures when working with unknowns?

Answer 48

1. contamination -->asceptic transfer | 2. Over growth & denature

Question 49

When you get your unknown, where will you keep your reserve stock?

Answer 49

fridge

Question 50

What does a positive catalase test look like?

Answer 50

bubbles

Question 51

What is the product that you are seeing when you have a positive catalase test?

Answer 51

O2

Question 52

What is the substrate for catalase?

Answer 52

H2O2

Question 53

The catalase test distinguides between 2 genera of Gram (+) bacteria. List them and then designate how they test for catalase.

Answer 53

Streptococcus --> catalase (-) | Staphylococcus --> catalase (+)