Microscopes Flashcards

0
Q

Total magnification

A

The enlarged size of the object verses it’s actual size when viewed using both ocular and objective lenses

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1
Q

Objective lenses

A

The 4 different lenses used to view the object in 4 different magnifications. 40x 200x, 400x, 600x

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2
Q

Magnification of ocular lense

A

10x

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3
Q

Magnification of scanning power lens

A

4x

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4
Q

Magnification of low power lens

A

20x

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5
Q

Magnification of medium power lens

A

40x

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6
Q

Magnification of high power lens

A

60x

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7
Q

Scanning power total magnification

A

40x

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8
Q

Low power total magnification

A

200x

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9
Q

Medium power total magnification

A

400x

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10
Q

High power total magnification

A

600x

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11
Q

The microscopes used in class are parfocal meaning…

A

As you change the objective lenses, the object will remain mostly in focus

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12
Q

Resolution

A

How clear an image of an object appears.

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13
Q

Contrast

A

Determining different parts of the object

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14
Q

Field of view

A

The area that can be seen with the microscope

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15
Q

Orientation

A

The image of the object is the result of mirrors, causing the image to be a mirror image of the object.

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16
Q

First thing you do before getting a microscope…

A

Clear a space in your work area

17
Q

When carrying a microscope…

A

Always use two hands. Support the arm with one hand, and the base with the other hand

18
Q

What is the only acceptable thing to clean a lens with?

A

Lens paper

19
Q

Always start on what power when using a microscope..

A

Scanning power

20
Q

What power should the microscope be on when you put it away?

A

Scanning power

21
Q

Never use coarse adjustment knob when?

A

When using medium and high power

22
Q

You may use which microscope

A

Only your assigned one #18

23
Q

Which objective lens will give you the largest field of view?

A

Scanning objective lens

24
Which objective lens do you always start viewing an object with?
Scanning objective lens
25
Which objective lenses do you use the fine focus knob with?
Medium and high power objective lens. Because lenses are very close to the slides.
26
Revolving nosepiece
The objective lenses are attached to the nose peice. Which allows you to change the objective lens in use. Rotate the nosepiece slowly, you will hear and feel a click when it is correctly in place
27
Arm
The part to hold when carrying a microscope
28
Base
Other hand is placed under the base when carrying, this part rests on the table
29
Coarse adjustment focus knob
A large knob on either side of the microscope. Used to move the stage up and down, to position the object in focus. This knob should be used only when the microscope is on the scanning or low power objective lenses
30
Fine adjustment focus knob
The smaller knob projecting out of the coarse adjustment knob. Makes very slight changes, used to bring the image of an object into sharp focus. Used on medium and high power objective lenses
31
Stage
The place where the slide is placed for viewing. There is a hole in the stage; the object to be viewed should be placed directly over the hole to allow light to shine through the object.
32
Stage clips and knobs
The metal clips holds the slide into place, and allow the slide to be moved easily to position or reposition an object
33
Condenser
The condenser focuses light
34
Iris diaphragm
This is located immediately under the stage. By moving this lever to the right or left, you can adjust the amt. of light that passes through the specimen and therefore the contrast
35
Light source
Comes from the base of the microscope, shines upward to illuminate an object
36
Reastat
Controls light. Little wheel. Needs to be turned away (all the way down) before putting the microscope back
37
Total magnification is figured out how?
Total mag.= ocular mag x object. mag
38
Pointer
The little needle that points to structures in the magnified image.
39
When viewing an object you should record what?
The total magnification