PAP'S Staining

Question 1

[3] Stains for PAP’s

[HOE]

Answer 1

1. Harris Hematoxylin
2. OG6 Stain
3. EA50

Question 2

for NUCLEAR STAIN.

[pap’s stain]

Answer 2

Harris Hematoxylin

Question 3

strong affinity to MATURE CELLS.

[pap’s stain]

Answer 3

OG6 Stain

Question 4

Orange green 6, 0.5 solution in 95% ETOH.

[pap’s stain]

Answer 4

OG6 Stain

Question 5

Phosphotungstic acid

[pap’s stain]

Answer 5

OG6 Stain
EA 50

Question 6

strong affinity for IMMATURE CELLS.

[pap’s stain]

Answer 6

EA 50

Question 7

It is comparable to EA 36.

[pap’s stain]

Answer 7

EA 50

Question 8

Differs from EA 50 or EA 36 only with respect the concentration of the light green stock solution.

Answer 8

EA 65

Question 9

Light green SF, yellowish 0.1% in 95% ETOH

[pap’s stain]

Answer 9

EA 50

Question 10

Vesicular nucleus:

[color]

Answer 10

Blue

Question 11

Produced by PROLIFERATING GRANULOSE the cells of the ovarian follicles, acts upon the superficial cells (mature cells).

[hormonal cytology]

Answer 11

Estrogen

Question 12

Produced by CORPUS LUTEUM formed after ovulation, acts upon the intermediate cells (immature).

[hormonal cytology]

Answer 12

Progesterone

Question 13

[3] Cells in Cytologic Examination

[SIP]

Answer 13

1. Superficial cells
2. Intermediate cells
3. Parabasal cells

Question 14

large (30-60um)

[cells in ce]

Answer 14

Superficial cells

Question 15

Polyhedral flat cells.

[cells in ce]

Answer 15

Superficial cells

Question 16

Cytoplasm: may be acidophilic or basophilic.

[cells in ce]

Answer 16

Superficial cells

Question 17

Presence of small dark pyknotic nuclei (less than 6um).

[cells in ce]

Answer 17

Superficial cells

Question 18

Medium large (20-30um)

[cells in ce]

Answer 18

Intermediate cells

Question 19

Polyhedral or elongated

[cells in ce]

Answer 19

Intermediate cells

Question 20

Cytoplasm: basophilic with vacuoles

[cells in ce]

Answer 20

Intermediate cells

Question 21

Vesicular nuclei (6-9um)

[cells in ce]

Answer 21

Intermediate cells

Question 22

Round to oval cells.

[cells in ce]

Answer 22

Parabasal cells

Question 23

Smaller than intermediate (15-25um).

[cells in ce]

Answer 23

Parabasal cells

Question 24

Thick Appearance: sunny-side up / fried egg.

[cells in ce]

Answer 24

Parabasal cells

Question 25

Have strong basophilic cytoplasm and vesicular nuclei (6-9um). [cells in ce]

Answer 25

Parabasal cells

Question 26

Found from 2 WEEK OF AGE TO PUBERTY, after childbirth, abortion or miscarriages and after menopause. [cells in ce]

Answer 26

Parabasal cells

Question 27

[3] Other cells that may be seen in PAP smear. [EED]

Answer 27

1. Endometrial cells 2. Endocervical cells 3. Doderlein bacilli

Question 28

Found during menstruation period, and 1-4 after the cessation of the period. [other cells in PAP's]

Answer 28

Endometrial cells

Question 29

If seen in a post- menopausal woman indicate a possible endometrial carcinoma. [other cells in PAP's]

Answer 29

Endometrial cells

Question 30

DENOTES: adequacy for Pap’s [other cells in PAP's]

Answer 30

Endocervical cells

Question 31

[4] Some normal cells that can be seen. [NRLT]

Answer 31

1. Neutrophils 2. RBC 3. Leptothrix sp. 4. Talcum

Question 32

Normally increase just before, during, and shortly after menstruation. [some normal cells that can be seen]

Answer 32

Neutrophils

Question 33

Seen during TRAUMATIC COLLECTION. [some normal cells that can be seen]

Answer 33

RBC

Question 34

Menstruation: Pap’s smear should be done 10 days after LMP. [some normal cells that can be seen]

Answer 34

RBC

Question 35

Increased in HIGH VAGINAL pH. [some normal cells that can be seen]

Answer 35

Leptothrix sp.

Question 36

Indirectly tells POSSIBILITY OF INFECTION. [some normal cells that can be seen]

Answer 36

Leptothrix sp.

Question 37

Usual contaminant. [some normal cells that can be seen]

Answer 37

Talcum

Question 38

OVOID BODIES. [some normal cells that can be seen]

Answer 38

Talcum

Question 39

[7] Abnormal Cellular Components of a PAP's smear. [CTG, KHCD]

Answer 39

1. Candida albicans 2. Trichomonas vaginalis 3. Gardnerella vaginalis 4. Koilocytes 5. HSV II 6. Cancerous lesion 7. Dysplastic cells

Question 40

BUDDING YEAST, seen among diabetics, taking oral contraceptives, immunocompromised patients, prolonged steroid therapy. [abnormal cellular components of a PAP's smear]

Answer 40

Candida albicans

Question 41

STD, pear shaped organism, granular cytoplasm, eccentric dark nucleus. [abnormal cellular components of a PAP's smear]

Answer 41

Trichomonas vaginalis

Question 42

[3] Quantitation in Vaginal Cytology [APM]

Answer 42

1. Acidophilic Index (A.I) 2. Pykontic Index (P.I) 3. Maturation Index (M.I)

Question 43

Percentage of cells that stain PINK-RANGE TO RED with pap’s and red in Shorr method. [quantitation in vaginal cytology]

Answer 43

Acidophilic Index

Question 44

“karyo-pyknotic index” [quantitation in vaginal cytology]

Answer 44

Kypontic Index

Question 45

Percentage of cells having shrunken, dark, small structures nucleic. [quantitation in vaginal cytology]

Answer 45

Pykontic Index

Question 46

PERCENTAGE PROPORTION of cells from. [quantitation in vaginal cytology]

Answer 46

Maturation Index

Question 47

[3] Cell Block [DPC]

Answer 47

1. Direct Filtration 2. Plasma Thrombin 3. Carbowax Method