1
Q
What is cancer?
A
Uncontrolled cell division that can invade other tissue and impede their function
2
Q
What is a tumour?
A
Any swelling Can be benign or malignant
3
Q
What is a neoplasm?
A
New growth not in response to any stimulus
4
Q
Define malignant
A
Metastatic potential is present This involves any neoplasm invading the basement membrane
5
Q
What is metastasis?
A
The spreading of a neoplasm to a different part of the body
6
Q
Give three pre-malignant stages
A
- Dysplasia - disordered growth with no stimulus (no invasion of basement membrane)
- Metaplasia - change of one cell type to another
- Hyperplasia - increase in cell number
7
Q
Metaplasia is a response to _____
A
Stress
8
Q
What can initiate metaplasia?
A
- Injurious or noxious stimuli
- Cytokines and cell signals
9
Q
Why are post-menopausal obese women at risk to hyperplasia (and cancer)?
A
Oestrogen will cause proliferation of the endometrium as part of the menstrual cycle. Cholesterol is similar in structure to oestrogen Obese women have high cholesterol which can cause proliferation in the endometrium Due to increased (an unnecessary) proliferations, these women have more “chances” for cells to begin to grown autonomously and for hyperplasia to occur
10
Q
What occurs in dysplasia that does not occur in either metaplasia or hyperplasia?
A
A genetic abnormality is developed
11
Q
What is carcinoma in-situ?
A
This is the final stage a neoplasm goes through before becoming malignant (invading basement membrane and spreading by metastases) This is the same as high-grade dysplasia
12
Q
How is the N:C (nuclear:cytoplasmic) ratio affected in malignant cells affected?
A
N:C ratio is high
13
Q
List some causes of cancer
A
- Genes
- Smoking
- Alcohol
- UV radiation, and other rdiation types
- Drugs
- Infections
- Obesity
- Burnt toast…supposedly Etc.
14
Q
What are Weinberg Hallmarks?
A
These are “bad decisions” made by a cell that are key to becoming malignant.
- Increased growth signals
- Growth suppression removed
- Avoiding apoptosis
- Achieving immortality
- Becoming invasive
- Making own blood supply (angiogenesis)
- Lose cellular DNA spellchecking
15
Q
What is Li-Fraumeni syndrome?
A
Genetic condition affecting the tp53 gene which codes for p53. This means sufferers from LFS are unable to stop excessive growth and attempt DNA repairs (or activate apoptosis)
16
Q
How does radiation cause cancer?
and What is Xeroderma pigmentosa?
A
Pyrimidine dimers are formed in DNA which are molecular lesions involving two consecutive bases on a single DNA strand to bind together ruining the normal base pairing Numerous instances can cause pyrimidine dimers in DNA to become overwhelmed
- its a genetic defect in nucleotide excision repair NER, suffer from numerous skin cancers
17
Q
Describe briefly the cell cycle
A
- Cyclin D activated CDK4 (cyclin dependent kinase)
- CDK4 phosphorylates Rb (retinoblastoma)
- Rb now unbinds from DNA allowing for DNA replication to occur - access to DNA is now possible
- Synthesis phase now occurs involving DNA replication
- M phase follows after G2 and the cell divides
- Cytokinesis is when the cell physically divided
18
Q
What are oncogenes?
A
A gene with the potential to cause cancer It involves increased growth
19
Q
What are tumour supressors?
A
These are genes preventing the pathway to cancer
20
Q
How can neoplastic cells evade DNA spellchecking?
A
Destroying spellcheck proteins such as P53
21
Q
What happens to a tumour in the bloodstream?
A
It will aggregate with platelets This means it will eventually slow down and stop within a blood vessel and grow in this new location
22
Q
Name 2 growth factors that can aid angiogenesis (for neoplasms)
A
- VEGF (vascular endothelial growth factor)
- PDGF (platelet derived growth factor)
(Angiogenesis is the formation of new blood vessels
23
Q
What are the three stages involved in the pathway of mutations and neoplasm development?
A
- Initiation - first mutation
- Promotion - accumulation of mutations (dysplasia)
- Persistance - malignant
24
Q
FISH is better than PCR for _____ genetic abnormalities
A
Large
25
PCR is ideal for _____ genetic abnormalites
Small
26
What are the functions of p53? (4)
1. Cell cycle arrest at G1
2. Increase levels of p21 to inhibit CDKs preventing Rb phosphorylation and hence DNA replication
3. Apoptosis activation when damage is too great
4. Activate repair mechanisms when damage is minimal
27
What are first principles in relation to identifying neoplasms?
These are quick decisions that can make an estimate as to whether the neoplasm appears malignant or not
28
How do benign tumour look?
1. round and smooth
2. homogenous (same type of cells)
3. symmetrical, organised
4. Normal N:C ratio
5. Encapsulated: Slow growth
29
How do malignant tumours look?
1. Not symmetrical
2. **Pleomorphism :**jagged edges
3. heterogenous
4. High N:C ratio: **_Hyperchromatia_ darkly stained nuclei, usually due to increased DNA content**
5. Fast growth
30
What is differentiation?
The process by which stem cells develop into mature cells types
31
What can poor cell differentiation indicate?
Neoplasms Potentially malignant
32
What is pleomorphism?
cells grow in multiple shapes and size forms of the "same" cell type - poor differentiation has taken place
33
What is hyperchromasia?
A cell which has a nucleus that stains very darkly - highlights large amount of genetic material suggesting fast growth and malignancy
34
Why does rapid cell division in neoplasms impact the number of mutations developed?
The more divisions that take place, the more chances there is for mutation
35
What is a neoplasm of the epithelium called?
Carcinoma - always malignant
36
What is a benign neoplasm of glandular cells called?
Adenoma
37
What is a benign neoplasm of squamous cells called?
Papilloma
38
What is a neoplasm of bladder epithelial cells called?
Urothelial cell carcinoma - always malignant
39
What is a malignant neoplasm of glandular cells called?
Adenocarcinoma
40
What is a malignant neoplasm of squamous cells called?
Squamous cell carcinoma
41
What are malignant neoplasms of connective tissue (mesenchyme) called?
Sarcomas
42
What are the names of benign/malignant neoplasms of fat tissue?
Benign - lipoma Malignant - liposarcoma
43
What are the names of benign/malignant neoplasms of bone?
Benign - osteoma Malignant - osteosarcoma
44
What are the names of benign/malignant neoplasms of cartilage?
Benign - enchondroma Malignant - chondrosarcoma
45
What are the names of benign/malignant neoplasms of skeletal muscle?
Benign - rhabdomyoma
Malignant - rhabdomyosarcoma
46
What are the names of benign/malignant neoplasms of smooth muscle?
Benign - leiomyoma
Malignant - leiomyosarcoma
47
What are the names of benign/malignant neoplasms of nerves?
Benign - neurofibroma (or schwannoma)
Malignant - peripheral nerve sheath tumour
48
What are the names of benign/malignant neoplasms of blood vessels?
Benign - haemangioma
Malignant - angiosarcoma
49
What are the names of benign/malignant neoplasms of the CNS?
Benign - gliomas
Malignant - named after benign version with malignant added in front
50
What is the name of malignant neoplasms of the skin?
Melanoma
51
What are names for blood neoplasms?
All are malignant Leukaemia Lymphoma
52
When referring to neoplasms what does "stage" refer to?
Distance from origin
53
In relation to neoplasms, what do karyotypes allow for?
Translocations to be discovered and defined
54
When referring to neoplasms what does "grade" refer to?
Level of differentiation
55
What is the difference between neoplasia and dysplasia?
Neoplasia - autonomous growth Dysplasia - disordered growth
56
What do cancers do to the body?
1. Compression/blockage of vessels/airways or ducts
2. Impacts tissue function
3. Haemorrhage - by infiltration
4. Infiltrating bone marrow can weaken the immune system
57
Tumours are very metabolically active using large amounts of energy, what is the name of the weight loss associated with this?
Cachexia
58
In which two ways can growing occur?
1. Hyperplasia - increase in cell number
2. Hypertrophy - increase in cell size
* e.g increase in size of myometrial smooth muscle cells increase in dimeters is more associate with hypertrophy.
* e.g Breast lobules have an increased number of cells under hormonal influence (mainly progesterone) to provide for normal lactation this shows as slight increase in brest size which is associated with hyperplasia
59
Whta causes hyperplasia?
An external stimulus
such as hormones
(when removed, growth is reversed)
60
What is atrophy?
The decrease in cell size
## Footnote
opposite to hypertrophy
61
What is metaplasia?
The reversible change from one mature cell type to another
62
Metaplasia occurs in a response to signals delivered to stem cells. What can some of these signals be due to?
* Cytokines
* Growth factors
* Other chemicals such as noxious stimuli
63
What are the four main stages in the cell cycle?
* G1
* S
* G2
* M
64
In the cell cycle what does G1 consist of?
* The cell increases in size as protein synthesis increases
* Cyclin dependent kinase 4 (CDK4) becomes activated by cyclin D due to phosphorylation and a complex is formed between the two
* After sufficient CDK4 is activated the cycle can proceed
* Retinoblastoma (Rb) can be phosphorlated by the complex and it comes free from E2F
* E2F can now act as a transcription factor
65
What is retinoblastoma?
A transcription inhibitor
| (normally binds to E2F)
66
What occurs in stage S of the cell cycle?
DNA replication occurs
67
What occurs in stage G2 of the cell cycle?
* The cell grows more due to increased protein synthesis
* P53 checks for errors in DNA replication
* It can attempt to fix errors or initiate apoptosis if the errors are too severe
68
Some cells are terminally differentiated and cannot divide or repair (e.g. neurones). What is a term to describe this?
Replicative senescence
69
What are telomeres?
They consist of a TTAGGG sequence at the end of a chain
They prevent the chromosome from degrading and "unravelling"
Telomere shortening is linked to ageing
70
What does the term "resolution" mean in terms of wound repair?
Returns tissue completely to normal
(occur when injury is minimal, there is good vascualr supply and the injurious agent is easily removed)
71
What is suppuration?
The production of pus
72
In terms of tissue repair, what does the term "organisation" mean?
Resolution cannot occur, and despite healing, scar tissue is present
This often occurs when the injury goes beyond the basement membrane
(occur if there is necrosis, large quantities of fibrin produced or poor blood supply)
73
What is granulation tissue?
When tissue becomes injured, the site is infiltrated with capillaries and myofibroblasts (muscle cells)
Collagen and smooth muscle are depositied
This tissue is ganulation tissue and has a red shiny appearance
74
Why does scarring occur and what is the consequence?
It occurs when myofibroblasts mature and produce collagen which causes the scar
This therefore means the functioning of that tissue segment is lost
75
Depending on what is found pathologically during autopsy, how can the recency or cause of death be determined?
Presence of neutrophils mean recent injury
Granulation tissue means there has been around 2 weeks since injury
Scarring can mean anything in excess of 6 weeks
76
What is scarring of the liver called?
Cirrhosis
77
What is the consequence of cirrhosis?
Liver failure
(toxins cannot be removed from the blood as efficiently, and proteins are not as easily synthesised)
78
Stages of Acute inflammation ?
Vasoconstriction,
vasodilation,
increased permeability of vessels,
stasis of red blood cells,
neutrophil margination
79
What are the cell types that are most dominant in both acute and chronic inflammation?
Acute - neutrophils polymorphs
Chronic - lymphocytes
80
What are the two categories of foreign bodies?
Both can potentially lead to granuloma formation
1. Exogenous - talc, asbestos, oil
2. Endogenous - keratin, bone, crystals
81
Which types of infections may lead to granuloma formation?
* Parasites
* Foreign bodies
* cancer e.g keratin production
* organ specific e,g Corhn's , hypersensitivity pneumonitis
* Mycobacterium (***TB***)
82
Tb granuloma is cause by what?
Caseousكاسياس necrosis (cheesy)
83
What are the two types of cell death?
1. Apoptosis
2. Necrosis
84
What are the three types of necrosis?
1. Coagulative necrosis
2. Liquefactive necrosis
3. Caseous necrosis
85
Describe coagulative necrosis
* Dead cells are consumed by enzymatic processes and other cells
* Ghost outlines exist since the nucleus is no longer present
* Cells are therefore no longer living so do not degrade properly
86
Describe liquefactive necrosis
* No cell structure is maintained
* Cells are liquefied
* This is normally associated with bacterial or fungal infections
87
Describe caseous necrosis
* Associated with Tb
* A Ziehl-Neelson stain can aid identification of caseous necrosis
* Involves granulomatous inflammation with central necrosis
88
What is the key difference between apoptosis and necrosis?
Apoptosis requires energy, necrosis does not
89
Apoptosis is normally physiological, but when may it be pathological?
* Injury
* Radiation damage
* Chemotherapy
* Viral infections
* Cancers
* After transplant
90
What are the two mechanisms for apoptosis?
* Extrinsic
* Intrinsic
91
Describe the extrinsic pathway for apoptosis
* It involves death receptor initiation
* FasL binds to death receptor Fas which activates caspases
* FADD, an adapter protein, bridges the gap between the Fas receptor and the caspases
* It is the death inducing signalling complex that undergoes conformational change when the Fas receptor is bound to caspases
* A caspase cascade occurs
92
Describe the intrinsic pathway for apoptosis
* Normally growth signals promote anti-apoptotic molecules in the mitochondrial membrane
* Growth signals become deactivated
* They are replaced by Bax and Bak - apoptotic regulators
* This increases the permeability of the mitochondria
* Proteins called cytochrome C escape inducing apoptosis
93
After apoptosis is induced, what then happens to the cell?
* Cells shrink by pyknosis (irreversible condensation of chromatin in the nucleus)
* The nucleus clumps and break up
* The cytoplasm breaks up forming blebs
* Macrophages remove debris
94
How is cellular ageing caused? (3)
1. Oxidative stress by free radical damage
2. Accumulation of metabolic by products such as lipofuscin (this accumaltes with age and is toxic to cells)
3. Telomere shorting lead to Finite cell divisions
95
What are telomeres?
Telomerase is **a large ribonucleoprotein complex responsible for progressive synthesis of telomeric DNA repeats (TTAGGG) at the 3′ ends of linear chromosomes**, thereby reversing the loss of DNA from each round of replication.
The TTAGGG sequence at the end of a chromosome
This prevents DNA unwinding
The number of repeats is reduced with each division and will eventually become depleted meaning cells can no longer divide
96
Why do stem cells never die?
Telomerase continually renews the TTAGGG repeats
This maintains telomere length
Telomerase is not active in normal cells, but can be in cancerous cells conferring immortality
97
After injury describe the vascular changes that occur
* Vasodilatation occurs
* Histamine and nitric oxide control this process
* This causes calor and rubor
98
What is the consequence of vasodilatation in vessels after injury?
Blood flow is slowed down (stasis) leading to white cell margination
99
4 stages of Leucocyte extravasation
Chemoattraction, rolling, tight adhesion, transmigration
SEE MORE IN THE NOTES
100
As a result of white cell margination, how do white cells interact with the endothelial wall?
* They will bind, via carbohyrate groups, to selectins which are luminal proteins on the endothelial wall
* This attraction has low affinity so the white cell rolls along the vessel wall
* The release of chemokines, by macrophages will activate white cells allowing them to bind to selectins more strongly and also to the vessel wall via integrin to ICAM-1 and VCAM-1 which results in much stronger affinity
* TNF and IL-1 will increase expression of ICAM-1 and VCAM-1
* Histamine and thrombin are released from inflammatory cells which increase selectin expression
* After coming to a halt, white cells undergo diapedesis
101
During inflammation, why do vessels become leaky?
White cells need to undergo diapedesis and reach the site of inffection or injury
102
"Leaky" vessels during inflammation is brought about by many mechanisms, what are these?
* Endothelial cell contraction (muscle tissue) - this is caused by histamine, bradykinin, substance P and leukotrienes
* Direct injury
* White cells - self harm will release damaging toxins
* Transcytosis - VEGF mediated, macromolecules are captured in vesicles on one side of the cell, drawn across the cell and ejected from the other side
* New vessel formation - new vessels are weaker and leakier, their production is mediated by VEGF
103
What is VEGF?
Vascular endothelial growth factor
104
What is chemotaxis?
Movement of cells along a chemical gradient migrating to the source of injury
105
What are the three phases of phagocytosis?
1. Recognition and attachment
2. Engulfment
3. Killing and degredation
106
Describe the recognition phase of phagocytosis
Bacterial surfaces express mannose receptors
Opsonins are antibodies or any other substance increasing suceptibility to phagocytes
The complement system will also surround foreign material
107
Describe the engulfment phase of phagocytosis
Pseudopods (arms) of the phagocyte surround the foreign material forming a vesicle termed a phagosome
The phagosome will then join with a lysosome and form a phagolysosome
108
Describe the killing an degredation phase of phagocytosis
Reactive oxygen species within lysomes kill pathogens
This ROS is created due to NADPH which oxidises oxygen
109
What are the 4 clinical features of inflammation?
1. Rubor (redness) / Erythema
2. Calor (heat)
3. Tumour (swelling)
4. Dolor (pain)
110
Why are neutrophils classed as polymorphs?
They have many lobes to their nucleus
111
3 type of Adaptations to increase demand on cells
* Increased demand – hyperplasia, hypertrophy
* Decreased demand – atrophy
* Altered stimulus - metaplasia (when body change its structure to try to adapt to the environment its in e.g squamous mucosa during Barrett's metaplasia )
112
when there is increase load on left ventricle what type of adaptation to increase demand on myofibers cells will happen
*Hypertrophy*
113
How doses immune system recognize pathogens that are hidden inside the cells
Through interacting with TOOL-LIKE RECTORS which present on infected cell surface with an antigen
114
How is endothelial contraction is involved in acute inflammation ?
Contraction of endothelial cells will lead to increase inter endothelial space which its the most important mechanism of vascular leakage.
during acute inflammation, the body's immune system responds to tissue damage or infection by releasing various mediators, such as histamine and bradykinin, which can cause blood vessels to dilate and increase blood flow to the affected area.
At the same time, these mediators can also cause the endothelial cells that line the blood vessels to contract, which can increase the gaps between the cells and allow fluid, immune cells, and other substances to leak out of the blood vessels and into the surrounding tissues.
This process of increased vascular permeability is important for allowing immune cells to reach the site of infection or injury and to help repair the damaged tissue.
115
what is the different between pathological hyperplasias and cancer ?
This where the growth control mechanisms become permanently dysregulated or ineffective and thus the process of pathological hyperplasias can not be reversed.
In many cases, pathologic hyperplasia could lead to cancers . For example, patients with hyperplasia of the endometrium are at increased risk of developing endometrial cancer
116
autophagy
is the process in which the starved cell eats its own organelles in an attempt to survive
117
what is Adaptation
**is reversible changes in number , size phenotype metabolic activity or function of the cell.**
**it can be**
**Physiologic adaptations** which is the response of cells to normal stimulations by hormones.
**Pathologic adaptations** are responses to stress that allow cells to modulate their structure and function, to escape injur.
e.g saving of nitrogen in the body instead of releasing it with urea when there is low level of it in the body
118
irreversible cell injury is associated with -----?
loss of membrane integrity. This allows intracellular enzymes such as AST and ALT to leak into the serum
119
what is the different between Hyperplasia and Hypertrophy ?
Increasing cell numbers requires activation of cell signalling pathways and the cell cycle
120
examples of reversible and irreversible injury
* Plasma membrane blebs, Ribosomal disaggregation, Chromatin clumping, and Mitochondrial swelling
* Nuclear fragmentation
121
What does coagulative necrosis mean
is a type of accidental cell death typically caused by ischemia or infarction. e,g Arterial thrombosis will lead to ischemia.
122
why does necrosis require no energy
because it is cell death by a pathological response such as **overwhelming chemical or physical insult**.
123
What process of cell death requires energy?
**A**
Apoptosis
124
where does apoptosis usually happen in cell cycle
G1 phase
125
G2 checkpoint?
M checkpoint?
Mitosis will not occur if DNA is damaged or not replicated
Mitosis stops if chromosomes are not properly aligned
126
What part of the cell is involved in cell aging?
**A**
Telomere
Shortening
127
What is a granuloma and when is it normally present?
**A**
- Collection of macrophages (in response to foreign bodies eg bone, asbestos, TB, parasites, syphillis, malignancy)
- FOUND in chronic inflammation
128
Sarcoma is malignant. True or false?
**A**
TRUE
129
Pathogenesis of atheroma?
**A**
- Fatty streak
- Fibrofatty plaque
- Proliferative atheroma
- Complicated atheroma
130
Aetiology of atheroma?
**A**
Endothelial injury
- Response to injury
- Macrophages + platelets
- Lipid accumulation
- Smooth muscle proliferation
131
Complications of atheroma?
**A**
Thrombosis
Aneurysm
Dissection
Embolism
Ischaemia
132
Thrombus definition?
**A**
Solid mass of blood constituents, formed within blood vessels
133
where is cytochrome c normally located ?
is located in the mitochondrial intermembrane/intercristae spaces
134
What are the causes of biological ageing
1. Oxidative stress free radical damage
2. Accumulation of lipofuscin (yellow)
3. Telomere shortening
135
only two terms that describe melginancy
carcinoma or sarcoma
136
mutation in the factor VIII gene that causes X-linked haemophilia causes what
Normal X-inactivation
mutation in the Factor VIII gene on the X chromosome causes hemophilia by impairing the body's ability to clot blood, and the severity of the condition depends on the amount of functional Factor VIII produced.
137
How does increased intracellular calcium contribute to cell death?
Hypoxia and reduced ATP can increase intracellular calcium levels, which can lead to cell death through apoptosis. Apoptosis can occur via an internal pathway (intrinsic) involving mitochondrial permeability or an external pathway (extrinsic) involving FAS. Small changes in calcium can activate the intrinsic pathway by releasing pro-apoptotic factors from mitochondria. Opsonisation and complement are involved in immune pathways that aid in phagocytosis and antibody activity.
138
the difference between GRANULATION TISSUE and GRANULOMA .
* two cell types are predominantly found in granulation tissue Endothelial cells and myofibroblasts
* GRANULOMA (composed of macrophages/histiocytes).
https://www.mypathologyreport.ca/
139
The form of necrosis that takes place in the CNS is termed
C. Liquefactive necrosis
140
Coagulative necrosis where cellular outlines are retained is often found in
cardiac muscle.
141
Resolution/restitution are forms of
repair
FOUNDATION MEDICINE
flashcards
Decks in class (23)
# Cards
-
ANATOMY Intro to Clinical language79
-
Physiology52
-
Embryology34
-
Cell replication83
-
Biochemistry from Principles Collated Flashcards190
-
Anatomy - Theory396
-
Genetics COPY from Principles Collated Flashcards132
-
Microbiology Cameron McCloskey181
-
Histology Cameron McCloskey157
-
Immunology Cameron McCloskey257
-
Biochemistry Cameron McCloskey187
-
Anatomy - Theory COPY396
-
Principles Anatomy Diagrams120
-
Pathology Cameron McCloskey141
-
Pharmacology Cameron McCloskey216
-
Evidence Based Medicine11
-
Public Health22
-
Human Factors4
-
Imaging15
-
Physiology COPY161
-
GI Y172
-
Genetics65
-
Immunology of the Gut72
