PERIPHERAL BLOOD & DIFF COUNT

Question 1

PERIPHERAL BLOOD FILM IS USED IN (2)

Answer 1

WHITE BLOOD CELL

PLATELET COUNT

Question 2

ALL SPECIMENS HAVE BEEN COLLECTED IN WHAT TUBE

Answer 2

ETHYLENEDIAMINE TETRAACETIC ACID (EDTA TUBE)

Question 3

PREFERRED TO THE POWDERED FORM BECAUSE IT MIXES MORE EASILY WITH BLOOD

Answer 3

LIQUID TRIPOTASSIUM EDTA

Question 4

HIGH QUALITY BLOOD FILMS CAN BE MADE FROM THE BLOOD IN EDTA WITHIN WHAT TIME

Answer 4

2-3 HOURS OF DRAWING SPECIMEN

Question 5

BLOOD FILMS THAT REMAIN AT ROOM TEMPERATURE FOR MORE THAN _____ have _______ blood cell artifacts.

Answer 5

5 HOURS

UNACCEPTABLE

Question 6

MAIN ADVANTAGES OF MAKING FILMS FROM BLOOD IN THE EDTA TUBE

Answer 6

MULTIPLE SLIDES CAN BE MADE AND THEY DO NOT HAVE TO BE PREPARED IMMEDIATELY AFTER BLOOD IS DRAWN

Question 7

IT PREVENTS PLATELETS FROM CLUMPING ON THE GLASS SLIDE

Answer 7

ETHYLENEDIAMINE TETRAACETIC ACID (EDTA TUBE)

Question 8

USE OF DIFFERENT ________ or no _______ may be useful

Answer 8

ANTICOAGULANT

Question 9

SOME PATIENT’s UNDERGOES AN IN VITRO PHENOMENON WHEN ANTICOAGULATED IN EDTA CALLED

Answer 9

PLATELET SATELLITOSIS

Question 10

LOW _________ AND FALSELY INCREASED ________ RESULT FROM EDTA INDUCED ________

Answer 10

PLATELET COUNT

WBC COUNT

PLATELET COUNTING

Question 11

ANOTHER SOURCE OF BLOOD FOR FILMS (2)

Answer 11

FINGER PUNCTURE

HEEL PUNCTURE

Question 12

TECHNIQUE USED IN PERIPHERAL FILM PREPARATION

Answer 12

MANUAL EDGE TECHNIQUE

Question 13

HOW MANY SLIDES YOU NEED TO MAKE A BLOOD FILM

Answer 13

1 slide for film slide

1 slide for spreader slide

Question 14

SIZE OF DROP OF BLOOD FROM A FINGER/HEEL/MICROHEMATOCRIT TUBE

Answer 14

2-3mm

Question 15

SIZE OF THE DROP OF BLOOD (2)

Answer 15

LONG AND THICK BLOOD FILM

SHORT AND THIN BLOOD FILM

Question 16

ANGLE OF PUSHER SLIDE/SPREADER SLIDE

Answer 16

30-45 DEGREES ANGLE

Question 17

MOVING PUSHER SLIDE FORWARD TOO SLOWLY ACCENTUATES POOR ______ BY PUSHING LARGE CELLS SUCH AS _______

Answer 17

LEUKOCYTES

MONOCYTES & GRANULOCYTES

Question 18

WHEN HEMATOCRIT IS HIGHER THAN NORMAL ANGLE SHOULD BE

Answer 18

LOWERED 25 DEGREES ANGLE

Question 19

WHEN HEMATOCRIT IS EXTREMELY LOW ANGLE NEEDS TO BE

Answer 19

RAISED

Question 20

WELL/MADE WEDGE PERIPHERAL BLOOD FILM

Film is ___ to ___ length of slide.
Film is ______,______,______
It provides ___ for examination.

Answer 20

2/3 to 3/4

Finger shaped, slightly rounded & feather edge.
Widest area

Question 21

LATERAL EDGES OF FILM ARE ____

Answer 21

VISIBLE

Question 22

WHEN SLIDE IS HELD UP TO THE LIGHT THE THIN PORTION HAS (2)

Answer 22

FEATHER EDGE & RAINBOW APPEARANCE

Question 23

ALL BLOOD FILMS SHOULD BE DRY QUICKLY TO AVOID_______

Answer 23

DRYING ARTIFACTS

Question 24

STAIN USED IN STAINING OF PERIPHERAL BLOOD FILMS

Answer 24

WRIGHT’S STAIN OR WRIGHT’S GIEMSA STAIN

Question 25

WRIGHT’S STAIN IS CONSIDERED AS POLYCHROME STAINS THEY CONTAIN (2)

Answer 25

METHYLENE BLUE EOSIN

Question 26

GIEMSA STAIN ALSO CONTAINS

Answer 26

METHYLENE BLUE AZURE

Question 27

FIXES THE CELL TO THE SLIDE

Answer 27

METHANOL

Question 28

OXIDIZED METHYLENE BLUE + EOSIN =

Answer 28

THIAZINE -EOSINATE COMPLEX

Question 29

STAINING REACTIONS ARE pH ________

Answer 29

DEPENDENT

Question 30

THE BUFFER THAT IS ADDED TO THE STAIN

Answer 30

0.05 M SODIUM PHOSPHATE pH 6.4 or AGED DISTILED WATER (placed in 24 hours) pH 6.4-6.8

Question 31

FREE METHYLENE IS ____ and STAINS ________ COMPONENTS SUCH AS

Answer 31

BASIC ACIDIC CELLULAR RIBONUCLEIC ACID

Question 32

FREE EOSIN IS _____ AND STAINS _____ COMPONENTS SUCH AS

Answer 32

ACIDIC BASIC HEMOGLOBIN & EOSINOPHILIC GRANULES

Question 33

ARE SO NAMED BECAUSE THEY HAVE CYTOPLASMIC GRANULES THAT HAVE NEUTRAL pH

Answer 33

NEUTROPHILS

Question 34

WRIGHT’S STAINING METHODS (3)

Answer 34

MANUAL STAIN AUTOMATED SLIDE STRAINS QUICK STAINS

Question 35

MACROSCOPICALLY STAINED BLOOD FILM: MICROSCOPICALLY BLOOD FILM:

Answer 35

PINK TO PURPLE RBCs APPEAR ORANGE TO SALMON PINK AND WBCs PURPLE TO BLUE

Question 36

STAINED CYTOPLASM OF NEUTROPHILS

Answer 36

PINK TO TAN WITH VIOLET GRANULES

Question 37

STAINED CYTOPLASM OF EOSINOPHILS

Answer 37

BRIGHT ORANGE REFRACTILE GRANULES

Question 38

THE BEST STAINING RESULT IS OBTAINED ON _____ BECAUSE THE BLOOD IT SELF ACTS AS A ____

Answer 38

FRESH SLIDE BUFFER

Question 39

SLIDES STAINED AFTER 1 WEEL OR LONGER TURN OUT TO

Answer 39

TOO BLUE

Question 40

OVERALL FILM QUALITY, COMOR, DISTRIBUTION OF CELS AND CHECK FOR FIBRIN STRANDS

Answer 40

10X LPO

Question 41

WBC ESTIMATE IS MADE HERE AND COINT WBCs IN 10 FIELDS AND FIND THR AVE. NO OF WBC/FIELD

Answer 41

40X HIGH DRY OBJECTIVE

Question 42

FORMULA FOR WBC ESTIMATE

Answer 42

AVE OF WBC / 10 FIELDS X 2000

Question 43

WBC DIFF COUNT IS MADE HERE. ALSO RBC AND PLATELET ESTIMATE

Answer 43

100X OIO

Question 44

USE OF BACK AND FORTH SERPENTINE OR BATTLEMENT PATTERN IS PREFERRED TO MINIMIZE DISTRIBUTION ERRORS AND ENSURE EACH CELL IS COUNTED

Answer 44

WHITE BLOOD CELL DIFFERENTIAL

Question 45

100 WBCs ARE COUNTED THROUGH THE USE OF THE

Answer 45

PUSHDOWN BUTTON COUNTERS

Question 46

IF COUNT IS HIGHER THAN 40X10^9/L

Answer 46

COUNT 200 cells

Question 47

IF COUNT IS HIGHER THAN 100 X 10^9/L

Answer 47

COUNT 300 or 400 CELLS

Question 48

RESULTS IN WBC DIFF COUNT REPORTED AS

Answer 48

PERCENTAGE (%)

Question 49

ALWAYS CHECK THE SUM OF PERCENTAGE IS ALWAYS ____

Answer 49

100

Question 50

IS AM IMPORTANT PART OF BLOOD FILM EXAMINATION INCLUDING CELL SIZE, SHAPE , COLOR, AND CELLULAR INCLUSION

Answer 50

RED BLOOD CELL MORPHOLOGY

Question 51

FORMULA PF PLATELET ESTIMATE

Answer 51

AVE. PLATELET COUNT / 10 X 20,000

Question 52

FORMULA IF RBC AND PLATELET IS ALTERED

Answer 52

AVE PLATELET COUNT/ 10 x RBC /200