WBC COUNT

Question 1

WHITE BLOOD CELL COUNT

THE NUMBER OF WBCs IN _____Liter or _____uL OF BLOOD

WHOLE BLOOD ANTICOAGULATED WITH _____ OR BLOOD FROM A _____ IS DILUTED WITH ______ OR_______

THE DILUTING FLUID LYSES THE ______ TO PREVENT INTERFERENCE

_____DILUTION OF BLOOD

NUMBER OF CELLS IN THE _____CORNER SQUARES ______

Answer 1

1 LITER OR 1 uL

EDTA OR SKIN PUNCTURE; 1% BUFFERED AMMONIUM OR 1% HYDROCHLORIC ACID

NONNUCLEATED RBCs

1:20

4 LARGE; 4mm^2

Question 2

WHAT ARE THE WBC DILUTING FLUIDS (3)

Answer 2

1-3% ACETIC ACID WITH GENTIAN VIOLET

1% HYDROCHLORIC ACID

TUERK’S

Question 3

CHARACTERISTICS OF A GOOD WBC DILUTING FLUID (5)

Answer 3

MUST BE A HYPOTONIC SOLUTION

MUST BE EASILY PREPARED

MUST BE READILY AVAILABLE

MUST BE A GOOD PRESERVATIVE

MUST BE CHEAP/AFFORDABLE

Question 4

WBC DILUTING FLUID IS

Answer 4

TO LYSE RBC EXCEPT NUCLEATED RBC

Question 5

MATERIALS NEEDED FOR WBC COUNTING (8)

Answer 5

EDTA ANTICOAGULATED BLOOD

WBC PIPETTE

1% HYDROCHLORIC ACID

ASPIRATOR

MICROSCOPE

IMPROVED NEUBAUER COUNTING CHAMBER

THICK COVERSLIP

TISSUE PAPER

Question 6

WBC PIPETTE PARTS

TOTAL VOLUME:

BULB:

STEM:

MARK:

Answer 6

IT HAS WHITE BEAD

11 UNITS

10 UNITS

1 UNIT

0.5 & 11

Question 7

PREPARATION OF DILUTED BLOOD

ASPIRATE BLOOD UP TO THE ____ OF WBC PIPETTE.

_____EXCESS BLOOD, BY NOT TOUCHING THE _____.

GRADUALLY BRING THE PIPETTE TO A _____ POSITION AND IMMEDIATELY MIXING CONTENTS. MAKE SURE PIPETTE IS SECURELY WITH THE ___ OR ____

DISCARD THE MIXTURE AN START FROM THE BEGINNING IF THE DILUTING FLUID IS ______ AND IF _____ ARE PRESENT INSIDE THE BULB

PLACE _____ ON TOP OF THE IMPROVED NEUBAUER COUNTING CHAMBER

RESHAKE PIPETTE, DISCARD FIRST _____ OF DILUTED BLOOD FROM THE PIPETTE

FILL THE COUNTING CHAMBER BY _____

LET THE COUNTING CHAMBER STAND FOR _____ TO ALLOW WBC TO SETTLE

FOCUS THE MICROSCOPE USING ____

Answer 7

0.5 MARK

WIPE OFF; TIP

HORIZONTAL; THUMB OR MIDDLE FINGER

BEYOND THE 11 MARK AND BUBBLES

THICK COVERSLIP

2-3 DROPS

CAPILLARY ACTION

5-10 MINUTES

LPO (LOW POWER OBJECTIVE)

Question 8

WBC FORMULA

Answer 8

WBC COUNT:NO OF WBC COUNTED/ 1/4 X 10 X 20

Question 9

CONVERSION FACTOR CONVENTIONAL TO SI UNIT

Answer 9

0.001

Question 10

SOURCES OF ERRORS (4)

Answer 10

FAILURE TO MIX BLOOD SPECIMEN

USING DILUENT CONATMINATED WITH BLOOD

BUBBLE IN THE THOMA PIPETTE

FAILURE TO MIX BLOOD AND DILUENT