Regs Flashcards
(240 cards)
1
Q
Dry bulb temp for Mouse, rat, hamster, gerbil, GP
A
20-26 C
68-79 F
2
Q
Dry bulb temp for cat, dog, NHP
A
18-29 C
64-84 F
3
Q
Dry bulb temp for Farm animals, poultry
A
16-27 C
61-81 F
4
Q
Dry bulb temp for rabbit
A
16-22 C
61-72 F
5
Q
IACUC requirements for PHS policy
A
Not less than five members
1) DVM
2) scientist
3) non-scientist
4) non-affiliated member
6
Q
What type of diet is suitable for studies conducted in compliance with FDA Good Laboratory Practice standards?
A
Certified, which are diets that have been analyzed for environmental contaminants
7
Q
What is the maximum number of voting IACUC members that can be associated with a single administrative unit?
A
3
8
Q
Who is exempt from USDA license?
A
- Retail pet stores that sell “nondangerous” pets
- Sell non-wild or non-exotic animals and makes <$500 gross
- Anyone who has ≤4 bitches/queens and sells only the offspring.
- Anyone who sells <25 dogs and/or cats per year for research, which were born and raised on his/her property. (May obtain voluntary license – Class A)
- Anyone who transports, buys, sells or breeds for food, fiber, or personal enjoyment.
- Anyone who breeds domestic pets for direct retail sales for the buyer’s own use, except dogs used for hunting, breeding, or security.
9
Q
Carbon monoxide should not exceed __ ppm according to Ag Guide.
A
150 ppm
10
Q
Ammonia should not exceed __ ppm according to Ag Guide.
A
25 ppm
11
Q
Hydrogen sulfide should not exceed __ ppm according to Ag Guide.
A
50 ppm
12
Q
Methane should not exceed __ ppm according to Ag Guide.
A
50,000 ppm
13
Q
OSHA permissible dust levels over 8-hour day are __ mg/m3 for respirable and __ mg/m3 for total
A
5 mg/m3 for respirable dust (smaller particles that can enter lungs)
15 mg/m3 for total dust (includes larger particles that may be inhaled but not enter lungs)
14
Q
According to the Animal Welfare Act and its regulations, official identification tag numbers used for cats and dogs may not be used more than once within what time period?
A
5 years
15
Q
According to AWA, fence for outdoor NHP vs marine mammal enclosure must be ___ feet tall and ___ feet from enclosure.
A
6 ft high (8 ft for marine mammal) and must be at least 3 ft from primary enclosure
16
Q
What should be included in a USDA certificate of a random-sourced dog obtained by a research facility from a dealer?
A
Description of dog, where dog/cat came from, dealer’s info, research facility’s info
17
Q
What would be exempt from USDA registration?
A
Federal government, elementary/secondary school labs, ag research
18
Q
How long should dealer, exhibitor, broker, operator of an auction sale, carrier, or intermediate handler keep records for?
A
1 year
(everyone but research facility, who must keep records for 3 years)
19
Q
According to the Animal Welfare Act and it regulations, what is the minimum frequency for sanitizing rabbit primary enclosures?
A
30 days
20
Q
Exceptions for 1 NHP per transport enclosure
A
Mother-infant
Established male-female
Compatible juvenile pair
21
Q
For dogs and cats Ambient temp must not be <__ or >__ for more than 4 hours. If the temperature is >__, must provide auxiliary ventilation.
A
45
85
85
22
Q
CO2 displacement rate for rodents per AVMA Euth Guidelines
A
30-70% volume per minute
23
Q
Cervical dislocation is AC for mice and rats < ___ grams and rabbits < ___ kg
A
200 g
1 kg
24
Q
Need to report any issues with nucleic acid work within ___ days to NIH OSP and IBC.
A
30
25
Institutional Biosafety Committee (IBC) must have no fewer than ___ members. At least ___ members must not be affiliated with the institution and must include at least ___ member representing the laboratory technical staff.
5
2
1
26
When do you need to appoint a biosafety officer?
1. Large volume (> 10 liters of culture)
2. BL3 or BL4 nucleic acid work
3. Any gene drive organisms
27
Which group sets containment levels for nucleic acid work?
IBC (Institutional Biosafety Committee)
28
What year was AWA enacted?
1966
29
What were AWA amendments? What was 1985 and 1990?
1970: not just dogs and cats
1976: added marine mammals; excluded mice, rats, birds, farm animals; no use of animals for fighting
***1985: no duplication, need training, exercising dogs, NHP psychological wellbeing, anesthesia/analgesia, multiple survival surgery, AWIC, IACUC***
***1990: holding period for dogs/cats***
30
Definition of animal by AWA
Any live/dead dog, cat, NHP, guinea pig, hamster, rabbit or such other warm-blood animal
Excludes
Birds (except if born in wild), rat of genus Rattus, mice of genus Mus bred for research
Horses not used for research
Farm animals to study farming
31
Class A vs B vs C license
A = dealer (breeder for purpose-bred)
B = dealer (random-source; involved in purchase/sale)
C = exhibitor
32
IACUC membership according to AWA
At least 3 members
Vet, scientist, unaffiliated
33
Hybrid wild x domestic animal is considered ___.
domestic
34
According to AWA, study area is place animal is for more than ___ hours. According to PHS, satellite facility is place animal is for more than ___ hours.
12
24
35
According to AWA, weaned means that an animal has become accustomed to take solid food and has so done, without nursing, for a period of at least ___ days.
5
36
AWR exemptions from licensing
1) Retain pet stores that sell "non-dangerous" pets
2) Sells non-wild, non-exotic animals and makes <$500
3) 4 or less bitches/queens and only sells offspring
4) Sells < 25 cats or dogs and they were born on property
5) For food, fiber, or enjoyment
6) Sells domestic pets for personal use by buyer (unless for hunting, breeding, or security)
37
How often to renew license vs registration for AWA?
License: annually
Registration: 3 years
38
Individual requesting license for AWA has ___ tries for the inspection. If does not succeed needs to wait ___ months to reapply. If license application is outright denied, have to wait ___ to apply again.
3
6 months
1 year
39
Notify AWA within ___ days of any changes to registration.
10 days (e.g. change in name, address, etc)
40
Research facility, which has not used, handled, or transported animals in ___ years, may be placed on inactive status, but ___ still must be filed.
2
annual report
41
Who appoints IACUC according to AWA?
CEO
42
According to AWA, IACUC cannot have more than ___ people from same department
3
43
According to AWA keep records for ___ years
3 years (research facility)
44
According to AWA, IACUC can use subcommittees consisting of at least ___ members.
2
45
If significant deficiencies are not corrected within timeframe, have ___ days to report this to USDA APHIS through IO
15 business days (i.e. there was an initial plan for correction and it is not followed through)
other things to report: suspensions of activities
46
Maintain USDA records for at least ___ years.
3
47
APHIS form for health certificates, program of veterinary care, acquisition, disposition, holding facility
Health certificate: 7001
Program of Veterinary Care: 7002 (required if no full-time AV)
Acquisition: 7005
Disposition: 7006
Holding facility: 7009
48
Dimensions for dog and cat circular and oblong tags. No reuse within ___ yrs. keep for at least ___
Circular: 1.25"
Oblong: 2 x 0.75"
5
1 yrs
49
Animal must be inspected within ___ days of health certificate
10
50
Holding period for dogs and cats for dealers/exhibitors at least ___ days and must include a ____. Holding period of animals obtained directly from privately-owned shelter ___ days. After this a second dealer would only need to hold for ___. Dogs or cats <120 days old that were obtained directly from the breeder need only be held for ___ (excluding transit time).
5 days
Saturday
10 days
24 hours
24 hours
51
How to identify puppies/kittens <16 weeks old
After weaning: tag, tattoo, plastic collar, microchip
If not weaned yet, only dam needs to be individually identified
52
What APHIS form is for non-dogs and cats:
record of animals on hand
record of acquisition/disposition/transport (for carriers and intermediate handlers)
7019
7020 (for dogs acquisition is 7005, disposition is 7006)
53
Carriers and handlers must attempt to notify the person receiving the animal (consignee) every __ hours for a period of __ hours. If still can't be reached, animal will be returned to the sender (consignor).
If the consignee is notified of the animal’s arrival and does not claim the animal w/in ___ hours, the animal will be returned to the consignor.
6 hours
24 hours
48 hours
54
Dealers, exhibitors, etc. cannot ship dogs or cats less than 8 weeks old and unweaned, UNLESS they are shipped to ____.
a registered research facility (they have no restrictions on age for shipping)
55
What is needed for health certificate for dog?
At least 6 months old
Vaccinated within 12 mo of arrival (distemper, hepatitis, leptospirosis, parvovirus, parainfluenza virus – DHLPP)
Dog at least 3 months old at time of Rabies vaccine; rabies vaccinated at least 30 days before arrival
56
Indoor temp requirements for dogs according to AWA
No <50 unless acclimated
Not <45 or >85 for more than 4 consecutive hours
Auxiliary ventilation if >85
57
Outdoor temp requirements for dogs vs NHPs
Dogs: <50F/10C only if acclimated
NHPs: Cannot go below 45
58
For dogs, suspended floors constructed of metal strands must be > ___ inch in diameter (___ gauge) or coated w/ plastic or fiberglass
1/8 inch (Saimiri >3/4 inch)
9 gauge
59
Space requirements for cats:
<4 kg =
>= 4 kg =
Height =
3 ft sq
4 ft sq
24"
60
Is cat litter pan part of floor space according to AWA and Guide?
AWA - yes as long as cleaned/sanitized
Guide - no
61
Kittens/puppies < ___ months may not be housed with adults other than the dam.
4
62
No more than ___ adult nonconditioned cats/dogs in the same room
12
63
According to AWA and Guide, when is resting surface part of floor space for cats?
If they can comfortably fit underneath it
64
Floor space and height requirements for dogs according to AWA
Measure nose to tail base in inches. Add six and square it then divide by 144 to get sq ft
Height 6" taller than the tallest dog
65
If space for each kitten or puppy is <__% of queen or bitch's need AV approval
5%
66
Exercise required for dogs > ___ weeks old.
What are 3 exceptions?
What are 2 exemptions?
12 wks old
Exceptions: 1) Bitch with litter
2) Dogs housed in groups where each dog has 100% of its required floor space
3) Dogs that have at least 2X their required space
Exemptions (reviewed annually): 1) IACUC-approved (scientifically justified) - REVIEW ANNUALLY
2) For health (unless PERMANENT exemption AV must review every 30 days)
67
How often to feed and water dogs, cats, and NHPs?
At least once a day
Must be ad lib for dogs unless approved by AV
Otherwise at least 1 hour twice daily
68
How often to sanitize primary enclosures and food/water receptacles for dogs, cats, guinea pigs, hamsters, NHPs?
Every 2 weeks
69
Carriers/intermediate handlers must not accept dog, cat, GP, hamster, or NHP more than ___ hours before scheduled departure, but can extend by ___ hours. For dogs and cats consignor (shipper) must certify that fed and watered during the ___ hours before giving to carrier/intermediate handler.
4
2
4
70
Dog or cat cannot be housed at <45F for more than ___ hours at terminal facility or > ___ minutes moving to/from. This is even if shipper has certified animal is acclimated to <___F within ___ days of shipment.
4 hours
45 minutes
50F
10 days
71
For transporting dogs and cats, there must be ventilation on two opposing walls that is at least ___% of the total surface area of all walls. In addition, what are ventilation requirements based on how many walls have ventilation? At least ___ of the ventilation area must be on the upper half of the enclosure. Projecting rims should make ___ inch gap when pushed against wall.
14%
1 wall - 90% of that wall (e.g. permanently affixed with only front ventilation opening)
2 walls - 16% of each wall
3 walls - 8% for opposing walls, 90% for third
4 walls - 8% of each wall
1/3rd
0.75"
72
How many dogs and cats can you transport in same enclosure for AIR transport?
No more than 1 dog or cat > 6 months
2 puppies if 8 weeks - 6 months and < 20 lbs otherwise 1 puppy
<8 weeks (unweaned or weaned) can be with dam
Exception: unlimited if shipping to research facility
73
How many dogs and cats can you transport in same enclosure for SURFACE or privately-owned air transport? How many GPs, hamsters, and rabbits?
No more than 4 dogs or cats > 8 weeks
15 guinea pigs or rabbits
50 hamsters
74
During transportation must offer dog, cat, and NHP food every __ hours and water every __ hours. If dog/cat < ___ weeks old and NHP <___year old must offer food every __ hours.
24; 12
16; 1; 12
75
How often to observe dog or cat during transport?
Every 4 hours (in plane only if accessible)
76
At terminal facilities, dogs and cats must have ventilation if >___F. Temp no >___F or <___F for __ consecutive hours. When moving to and from terminal facility, temp cannot > ___F for more than ___.
85
85, 45, 4 hours. Only <45 if certified acclimated! If not certified, only 45 minutes.
85, 45 min (can't really provide ventilation)
77
Temperature for guinea pigs and hamsters per AWA
60-85 (no less even if acclimated)
78
Which animal cannot be housed outdoors according to AWA?
Hamsters (because of LCMV)
79
Space requirements for guinea pigs
<350 g: 60 sq in
>350 g: 101 sq in
Nursing with litter: 101 sq in
80
Height requirement for guinea pigs and hamsters?
Guinea pigs: 7 in
Hamsters: 6 in
81
Space requirements for hamsters
<60 g: 10 sq in
60-80 g: 13 sq in
80-100 g: 16 sq in
>100 g: 19 sq in
Nursing with litter: 121 sq in (25 for dwarf)
82
Certification of being acclimated to lower temp must be within ___ days of transport.
10
83
For dog/cat housing need certification acclimated to temp <___ and for transportation of dogs/cats/hamsters/rabbits <___.
50
45
84
How do GP/hamster ventilation requirements for shipping differ from dog/cat?
Need at least 1/3 of ventilation in lower half (in addition to 1/3 in upper half like dog/cat; this is the same as marine mammals)
Same ventilation surface area requirements, same projecting rims to allow 0.75" of space
85
For indoor housing, auxiliary ventilation when temp > __ for dogs, rabbits, NHPs and __ for hamsters. For transportation, auxiliary ventilation when temp > __ for dogs and __ for hamsters/GPs/rabbits/marine mammals
85 (but Guide temp for rabbit 61-72)
75
85
75
86
Guinea pigs, hamsters, and rabbits need food if transported for more than ___ hours.
6
87
Indoor facilities for what species do not need to be heated and no lower limit for temp?
Rabbits
88
For outdoor housing, rabbits need active cooling e.g. sprinkler when temp > __F. Vs indoor housing need aux ventilation when temp > __F.
90
85
89
For outdoor dogs, put bedding in shelter if <___, extra bedding if <___.
For outdoor rabbits, provide shelter if temp <___ (but if transporting < ___)
50F/10C
30F/1.7C
40F, 50F
90
Need nest box for rabbit with litter <__ months old
1
91
Floor space requirements for rabbits according to AWA
Height 14" (16" per Guide)
<2 kg: 1.5 sq ft
2-4 kg: 3 sq ft
4-5.4 kg: 4 sq ft
>5.4 kg: 5 sq ft
With litter:
<2 kg: 4 sq ft
2-4 kg: 5 sq ft
4-5.4 kg: 6 sq ft
>5.4 kg: 7.5 sq ft
92
Perimeter fence for outdoor NHP enclosures must be at least ___ ft high and must be at least ___ ft from primary enclosure. The purpose is to prevent entry of ___, ___, and ___.
6
3
Raccoon, skunks, and dogs
93
If NHPs are restrained for >___ hours, they must be provided daily unrestrained activity for at least ___ hour during the period of restraint, unless continuous restraint is required by the research proposal.
12
1 continuous hour
94
NHP or dog may be exempt from enrichment plan by AV, but needs to be reviewed every ___ days.
30
95
For transportation, only one NHP per enclosure except...
1) Mother and nursing infant
2) Established male-female pair (but not in estrus)
3) Compatible pair of juveniles
96
Food for marine mammals can be in iced cooler for no longer than ___ hr
12
97
Vertical air space above pool for marine mammals must be at least ___ft (___ m).
6 ft/1.83 m
98
The enclosures of which 2 types of marine mammals shall be kept free of ice
1) Cetaceans
2) Sea otter
99
The minimum space of pool for marine mammal is based on what 4 factors?
1) Minimum horizontal dimension (MHD)
2) depth
3) volume
4) surface area
100
Pools for marine mammals can be smaller than minimum allowed (e.g. treatment, training) but if > __ weeks need AV approval on a ___ basis
2 weeks
Weekly
101
Marine mammals should be fed ___ times daily. Frozen fish removed from freezer must be fed within __.
1
24 hours
102
What is limit for coliform bacteria for marine mammals per 100 mL of water?
1000 MPN (Most Probable Number) per 100 mL
Test weekly
103
How long to keep records for feeding, water testing, necropsy for marine mammals?
Feeding: 1 year
Water testing: 1 year
Necropsy: 3 years
104
Salinity for marine mammals should be __ to __ parts per thousand
15 to 36
105
Must perform complete necropsy on all captive marine mammals within ___ hours.
48
106
Must certify acclimated if housing temp < ___ (dogs/cats) and < __ (marine mammal)
Must certify acclimated if transport temp < __ (dogs/cats/NHPs) or < __ (hamster/rabbit)
50
45
50
45
107
Temp range and ventilation requirements for transport of marine mammal
45-75
Need ventilation on all 4 walls with each 16% of surface area of the wall (same as dogs except they had 16% of each of 2 walls)
108
Which marine mammal needs at least 1/2 of floor as ice or ice water to keep haircoat moist?
Sea otter
109
If Secretary believes someone violated AWA/AWR, can suspend license for up to ___ days.
21
110
Floor space requirements and height for NHPs per AWA vs Guide
AWA
Group 1 (<1 kg) - 1.6 sq ft, 20"
Group 2 (1-3 kg) - 3 sq ft, 30"
Group 3 (3-10 kg) - 4.3 sq ft, 30"
Group 4 (10-15 kg) - 6 sq ft, 32"
Group 5 (15-25 kg) - 8 sq ft, 36"
Group 6 (>25 kg) - 25.1, 84"
Guide differences
Group 1 (<1.5 kg) - 2.1
Group 2 (1.5-3 kg)
Group 5 (15-20 kg) -
Group 6 (20-25 kg) - 10
Group 7 (25-30 kg) - 15
Group 8 (>30 kg) - 25
111
If rabbits housed on solid-flooring, ___ must be provided and cleaned every ___.
Litter
Once a week
112
5 considerations for environmental enhancement plan for NHPs according to AWA
1. Restraint devices
2. Exemptions
3. Social housing
4. Special considerations
5. Environmental enrichment
113
According to AWA, licenses authorize increments of ___ animals on hand at any single point in time during the period of licensure.
50
114
According to AWR/Guide/PHS, if full committee review of a protocol is NOT requested, what is the minimum number of IACUC members that may review and approve the protocol (DMR)?
ONE
115
Does IACUC member abstaining due to conflict of interest count towards quorum?
NO
116
Who registers with USDA? How often is renewal?
Research facility, carrier, intermediate handler, and unlicensed exhibitor
carriers and intermediate handlers are registered, not licensed
(every 3 years)
117
PHS assurance every ___ years.
up to 5 years
118
Dog and cat ID tag can be made of made of ____
***durable alloy (brass, bronze, steel, durable plastic) or aluminum***
no nickel - not durable enough, allergic reactions
119
Small breeders with less than ___ breeding females may get voluntary license (Class __)
4
A
120
Certain NHPs require special attention with regard to enrichment of their environment:
1) Infants and juveniles
2) Showing signs of being in distress
3) Activity restricted per protocol
4) Can't see/hear other NHPs of their spp
5) Great apes >50 kg (110 lbs)
121
According to the Animal Welfare Regulations, what option is NOT available to a designated member of an IACUC when reviewing a proposed research activity?
Withhold approval (can only approve, approve w/ modifications, or request FCR)
122
Definition of research facility by AWA
school (except grade school), institution, org or person that uses or intends to use live animals in research or testing (does NOT mention teaching)
123
According to AWA when is dedicated surgical facility required?
For MAJOR survival surgery
124
Annual report for USDA must be signed by ___ or ___. Which animals need to be reported?
CEO or IO
Report animals prospectively (before, during, and after use)
125
According to the Animal Welfare Act and its regulations, who is responsible for ensuring that all scientists, research technicians, animal technicians, and other personnel involved in animal care, treatment, and use are PROVIDED INSTRUCTION (trained) on the proper use of anesthetics, analgesics, and tranquilizers for animal species used by the facility?
Research facility
126
The Animal Welfare Information Center (AWIC) and ___ are two databases created by the ____, a unit within the U.S. Department of Agriculture.
AGRICOLA
National Agricultural Library (NAL)
127
Which APHIS form is the annual report?
7023
128
IACUC requirements per Guide
No less than 4 members
DVM, scientist, unaffiliated, nonscientist
129
How often should program review and facility inspections be according to AWA, Guide, and PHS? Afterwards, a report should be sent to ___.
AWA/PHS: q6months
Guide: annually
IO
130
According to Guide, body weight for animals on fluid regulation should be recorded ___ per week.
1X
131
What is the thermoneutral zone for mice, rats, and gerbils according to the Guide?
Mice: 26-34 C
Rats: 26-30 C
Gerbils: 28-32 C
132
Increased ammonia in rodent caging associated with ___ relative humidity.
High
133
Direct exposure to drafts should be avoided as 20C air at 60 lfpm (18.3 m/min) has a cooling effect of ___C
7C
134
Exhaust air to be recycled should be filtered at min w/ ____% ASHRAE efficient (dust spot efficient) filters before its recycled
85-95
135
Noise exposure to >___ decibels can cause eosinopenia, increased adrenal gland weights, reduced fertility in rodents, and increased BP in NHP. Need hearing conservation program > ___ decibels. Must not go above ___ decibels.
85
85
90
136
Floors should be sloped ___" per ft towards trough; in trough should slope ___” to ft towards drain
3/16th inch
1/4 inch
137
Guide space requirements for mice
<10 g: 6 sq in
10-15 g: 8 sq in
15-25 g: 12 sq in
>25 g: 15 sq in
Female w/ litter: 51 sq in
Height: 5"
138
Guide space requirements for rats
<100 g: 17 sq in
100-200 g: 23 sq in
200-300 g: 29 sq in
300-400 g: 40 sq in
400-500 g: 60 sq in
>500 g: 70 sq in
Female w/ litter: 124 sq in
Height: 7" (like guinea pig)
139
Guide space requirements for dogs
<15 kg: 8 sq ft
15-30 kg: 12 sq ft
>30 kg: 24 sq ft
140
Guide space requirements for pigeons
0.8 sq ft per animal
141
Guide space requirements for quail
0.25 sq ft per animal
142
Guide space requirements for chickens
<0.25 kg: 0.25 sq ft per animal
0.25-0.5 kg: 0.5
0.5-1.5 kg: 1
1.5-3 kg: 2
>3 kg: 3
143
Space requirements for chimps according to Guide?
Juvenile (<10 kg): 15 sq ft
Adult (>10 kg): 25 sq ft
144
Space requirements for sheep and goats per Guide
1 animal per enclosure
<25 kg: 10 ft2
25-50: 15 ft2
>50: 20 ft2
2-5 animals
<25 kg: 8.5
25-50: 12.5
>50: 17.0
>5 animals
<25 kg: 7.5
25-50: 11.3
>50: ≥15.0
145
Space requirements for swine according to Guide
1 animal
< 15: 8.0
< 25: 12.0
< 50: 15.0
< 100: 24.0
< 200: 48.0
> 200: ≥60.0
2-5 animals
< 25: 6.0
< 50: 10.0
< 100: 20.0
< 200: 40.0
> 200: ≥52.0
>5 animals
< 25: 6.0
< 50: 9.0
< 100: 18.0
< 200: 36.0
> 200: ≥ 48.0
146
Space requirements for cattle per Guide
1 animal
<75: 24.0
<200: 48.0
<350: 72.0
<500: 96.0
<650: 124.0
>650: ≥144.0
2-5
<75: 20.0
<200: 40.0
<350: 60.0
<500: 80.0
<650: 105.0
>650: ≥120.0
>5
<75: 18.0
<200: 36.0
>350: 54.0
<500: 72.0
<650: 93.0
>650: ≥108.0
147
Guide space requirements for horses and ponies
Horses: 144 sq ft per animal
Ponies:
1-4 animals: 72.0
>4 animals: If <200 kg 60, if >200 kg 72
148
What type of diet contains the most elemental ingredients available, such as aa and specific sugars?
Chemically defined
149
Natural-ingredient diets: stored < ___C/F and <___RH
Purified and chemically-defined diets: stored ___
<21°C (70°F), <50% RH
4°C (40°F)
150
According to the Guide:
Sanitize enclosures and accessories, such as tops, q___wk and solid-bottom caging, bottles, and sipper tubes q___wk
2
1
151
Disinfection with hot water: ___ @ 180°F; ___ @ 161°F; ___ @ 143°F)
1 second
15 seconds
30 minutes
152
Toe-clipping in rodents for identification only when no other means is feasible and only in neonatal mice up to ___ days of age
7 days old
153
3 types of life support systems for aquatics
recirculating, flow-through, and static
154
Space requirements for zebrafish and frogs per Guide
Zebrafish: 5 fish per L
Frogs: 1 frogs per 2 L
155
In general, housing temperature should be ____ than the ____ critical temp
lower, lower
156
Examples: Why house African and Asian primates separately? Why house OWP and NWP separately?
African carry SHFV, SRV that are subclinical in African but cause dz in Asian primates
Squirrel monkeys, tamarins herpesviruses --> owl monkeys
157
How does definition of major survival surgery differ between AWA and Guide?
Both refer to exposing body cavity and impairment of function, but Guide also includes extensive tissue dissection/transection
158
Definition of euthanasia
Act of killing animals by methods that induce rapid unconsciousness and death w/out pain or distress
158
Definition of distress per Guide
Distress: aversive state in which an animal fails to cope or adjust to various stressors with which it is presented
159
Width of corridor and size of doors according to Guide
6-8 feet wide
42 x 84 inches
160
Drains at least ___ inches in diameter; at least ___ inches in Ag animal, NHP, or dog facilities
4
6
161
Set point for temp should be +/- ___C/___F. RH should be +/-___% of set point.
1C/2F
10%
162
____ type of HVAC system most common. ____ systems allow vent rates to be set in accordance w/ heat load
Constant-volume system
Variable-volume system
163
Most HVAC designed to maintain temp/hum w/in ±____% variation of average highs and lows for a geographic area
5
164
Dedicated refrigerated storage to keep carcasses <___C/___F
7C
45F
165
Fire alarms should be what frequency to avoid disruption of rodents?
<1 kHz (low end of hearing range for mice)
166
Surgical spaces should be (positive/negative) pressure.
POSITIVE
167
Which document states “Unless the contrary is established, investigators should consider that procedures that cause pain or distress in human beings may cause pain or distress in other animals”?
U.S. Government Principles for the Utilization and Care of Vertebrate Animals Used in Testing, and Training
168
Who is responsible for ensuring training is adequate for experimentation/surgery/anesthesia/analgesia? Who is responsible for overseeing and doing the training?
Research facility
IACUC and AV
169
2 methods for humidification
steam injectors or atomizing water into the air stream
170
According to the Guide, responsibility for review and investigation of animal welfare concerns in the U.S. rests with the ___ and ___. Responsibility for the Program rests with ___.
IO and IACUC
IO
171
________ is responsible for providing appropriate resources to support personnel training, and __________ is responsible for providing oversight and for evaluating the effectiveness of the training program.
The Institution
IACUC
172
Guide: When applicable, the IO must submit a request to ___ and receive approval in order to allow a regulated animal to undergo multiple major survival surgical procedures in separate unrelated research protocols.
USDA/APHIS
173
What provides statutory mandate for PHS policy?
Health Research Extension Act of 1985
174
US Govt Principles from ___ in ____.
IRAC
1985
175
US Government Principles (9)
1. In accordance with AWA
2. Justify animal use
3. Appropriate species, minimize number, consider alternatives
4. Minimize pain/distress: Unless otherwise indicated, procedures that cause pain or distress in humans may cause pain or distress in other animals
5. Sedation, analgesia, or anesthesia. No paralytics in unanesthetized animal.
6. Humane endpoints, appropriate euthanasia
7. Good husbandry/vet care
8. Qualified personnel, proper training
9. IACUC decides on exceptions
176
Abstentions from voting ___ affect quorum
Recusal of a member due to conflict of interest ___ affect the quorum
do not
does
177
According to PHS FAQs, can IO approve, suspend, or reinstate animal activities?
YES: can suspend
NO: can't approve or resinstate them
178
Program description as part of Assurance should include what 8 components?
1. List all major branches (components) of the institution
2. Lines of authority/responsibility for administering the program
3. Qualifications of vet and % time will contribute
4. IACUC member list: names, position titles, credentials - may replace names of members (other than Chair and DVM) with number or symbol
5. IACUC procedures to fulfill requirements of policy
6. Occ Health program
7. Synopsis of training in humane animal care and use
8. Size of facility, species, census
179
What are the three results of IACUC review
Approve, require modifications (to secure approval), or withhold approval
180
Importers must notify CDC at least ___ days before importing a shipment of NHPs.
7
181
Which AWA amendment established holding periods for dogs and cats?
1990
182
Three primary standards used by AAALAC
1. Guide
2. Ag Guide
3. European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes
183
How is AAALAC governed?
1 representative from each Member Organization; serve for 3 year term
184
What is a Program Status Evaluation (PSE)?
Through AAALAC
Assessment of non-accredited program before formal application
185
How often to submit report to AAALAC?
Annually (due Dec 1st)
186
4 outcomes for new applications to become AAALAC-accredited
1. Full accreditation
2. Accreditation with condition (need to correct mandatory item)
3. Provisional status (NOT accredited) --> up to 24 months to correct mandatory item
4. Withhold accreditation
187
5 outcomes for accredited institutions after site visit
1. Continued full accreditation (only category with FULL accreditation)
2. Conditional full accreditation (need to correct mandatory item)
3. Deferred accreditation --> 2 months to correct mandatory item; if not will become probation
4. Probation --> 12 months to correct mandatory item (still accredited)
5. Revoke accreditation
188
Which OSHA form is a log of work-related illness and injury? What form needs to be filled out for each entry in the log?
OSHA Form 300
OSHA Form 301 = Injury and Illness Incident Report
189
At the end of each calendar year, OSHA Form ____ must be completed and certified by a company executive as correct and complete and posted in the workplace where notices to workers are usually posted. How long does it need to be posted?
300-A
Posted for three months from Feb 1 - Apr 30
190
The National Fire Protection Association (NFPA) has classified fires into what 5 types
Class A - wood/paper (leaves ***ash***)
Class B - liquids (they ***boil***)
Class C - electrical (eg ***computers/circuits***)
Class D - Mg/Na/K (need ***dirt***/suppressant to put out)
Class K - cooking oil
191
What is the difference between combustible and flammable?
Combustible = flash point 100-200
Flammable = flash point <100 (more dangerous bc includes room temp!)
192
UV radiation (UV-B, UV-C) reacts with the vapors of chlorinated solvents (eg trichloroethylene, trichloroethane, chlorofluorocarbons) to produce ____, a potent lung irritant.
Phosgene
193
What group classifies lasers?
American National Standards Institute (ANSI)
194
What are the five classes of lasers according to ANSI?
Class I: no hazardous radiation
Class II: not enough power to injure someone accidentally but can cause injury ***if viewed for extended periods of time***
Class IIIa: can cause injury if viewed with a ***viewing device (binoculars; concentrated beam)***
Class IIIb: can cause injury if ***viewed directly, if reflected off mirrors can cause damage***
Class IV: fire hazard
195
What are the two classifications of ionizing radiation?
1. Particulate - alpha (2 neutrons/2 protons) or beta (electrons) ***alpha can't cross skin***
2. Non-particulate - x rays, gamma rays
196
What is red, yellow, white, and blue section of National Fire Protection Association (NFPA) Diamond for chemicals?
Red: Flammability
Yellow: Reactivity/stability
Blue: Health
White: Specific
197
UV-A, B, and C
UV-A: 320-400 nm --> skin pigmentation
UV-B: 280-320 nm --> keratitis, cataract, erythema
UV-C: 180-280 nm --> germicidal (260 most bactericidal)
198
Maximum weight a person can lift per NIOSH
51 lbs (8-12 if repeat load lifting and torso lifting)
199
Name of blood test used to diagnose allergies by measuring the amount of specific IgE antibodies in blood
RAST (radioallergosorbent test)
200
What is main allergen from mice and where is it produced and excreted?
Mus m1
Produced in liver, excreted in URINE
100X more in urine than serum
4X more in males
201
What is second most prevalent allergen from mice?
Mus m2 (in fur, dander)
202
What is the main allergen from rats?
Rat n1 A (alpha-2u globulin)
Excreted in urine, saliva
203
What is the main allergen from rabbits?
Ory c 1
In fur
204
Main allergen from cats
Fel d 1
Produced in sebaceous glands and saliva
In saliva, skin, fur of ALL CATS
205
Main allergen from dogs
Can f 1
Saliva, dander, fur
206
Mechanism of bird hypersensitivity
Mediated by IgG not IgE!
207
Exposure limits for formaldehyde TWA and short-term exposure
TWA: 0.75 ppm
Short term Exposure: 2.0 ppm
208
What wavelength of UV light is filtered by glass window?
<320 nm (UV-B and UV-C)
209
What is "creeping eruption"?
Anatrichosoma cynomolgi
In NHPs
Nasal mucosa —> hands/feet with pruritus
Zoonotic
209
What zoonotic roundworm causes SQ granulomas and anemia in OWP?
Oesophagostomum
210
What is Onithonyssus bacoti a vector for?
Typhus
Rickettsia
Plague
Tularemia
Q fever
Zoonosis leads to dermatitis
211
What is the vector for rickettsialpox, a disease caused by the bacterium Rickettsia akari?
Liponyssoides sanguineus (a mouse louse)
212
What provides guidance for chemical fume hoods? What should face velocity be?
American National Standards Institute (ANSI)
80-100 lfm
213
What provides guidance for certifying BSCs?
NSF
(later became NSF/ANSI Standard 49)
214
What organization was the first to develop guidelines for education and training in laboratory animal medicine and is still a significant resource used today when it comes to identifying practices that provide for excellence in the welfare of animals used in research, education, testing, and the production of pharmaceuticals?
ILAR (The Guide)
215
What organization issued a report in 2011 that established criteria to guide the current and future research use of Pan spp?
Institute of Medicine (old name) - now The National Academy of Medicine
216
According to PHS policy, what is Category 1 vs 2 institution?
Category 1 – Accredited by AAALAC
Category 2 – Evaluated by the Institution with the most recent semiannual report of the IACUC evaluation submitted to OLAW with the assurance
217
How is NIOSH different from OSHA?
NIOSH in CDC within Dep of Human Health and Services HHSa
OSHA within Dep of Labor
218
Hierarchy of controls
Elimination
Substitution
Engineering controls
Administrative controls
PPE
219
Primary guinea pig allergen
Cav p 1
dander, saliva, urine
Recently considered the most allergenic laboratory animal species
220
Prevalence and cause of asthma in laboratory animal workers
Up to 10%
Causes: exposure to allergens, airborne endotoxin in bedding/dusts, genetic susceptibility
Other risk factors for respiratory dz in animal workers: age, smoking, atopy, skin test reactivity to non-animal allergens
221
What is the current standard for safety glasses?
ANSI/ISEA Z87.1
222
What organization certifies respirators?
NIOSH
223
What BSL/ABSL for Brucella?
BSL-3
224
What does HEPA stand for?
High Efficiency Particulate Air
225
What are two compounds used for leak testing of HEPA filters?
Di-octyl phthalate (DOP) and poly-alpha-olefins (PAO) are both used in filter leak testing
Now recommend PAO bc DOP carcinogenic
226
Benefits of gamma irradiation
Passes through solid objects
Less reduction in nutritional value of food than autoclaving
Lower consumable cost
227
Who has the responsibility for implementing the occupational health and safety program by providing training and necessary personal protection?
Supervisor
228
The distance that beta particles can travel depends on their source: in air, beta particles emitted from which radioisotope will travel the farthest (i.e. has the most energy)?
Phosphorous-32 (P-32)
229
Which of the following laws enacted by Congress require that institutions provide occupational health and safety training for staff that care for or use animals?
HREA --> PHS
Also Food Security Act?
Also a must in the Guide but that's not law
230
If the frequency of sound is below ___ KHz, it is covered by the OSHA noise standard
20 (below which we can hear)
231
Which document mandates developing and maintaining a written hazard communication program for the workplace that includes a list of the hazardous chemicals in use in the facility; proper labeling of chemicals in use; and employee access to Material Safety Data Sheets?
Hazard Communication (HAZCOM) Standard - written by OSHA
232
What are the minimum requirements for the use of a respirator per the Guide?
Medical Clearance and Respirator Fit Testing
233
What is term for person from USDA who comes to visit?
VMO (Veterinary Medical Officer)
234
USDA form for exercise plan for dogs and environmental enhancement plan for NHPs
7013
7050
235
Can you use NIH funds to purchase dogs from Class B dealers?
No (started to be enforced in 2015)
236
First Guide came out in ___, was created by the ___, and was originally named ___.
1963
Animal Care Panel
Guide for Laboratory Animal Facilities and Care
237
What USDA housing group are baboons?
Group 5 (between 15-25 kg)
238
How often to perform facility inspection and program review per AWA, Guide, and PHS?
AWA and PHS: q6months
Guide: annually
