Retics Lab Flashcards

1
Q

Young form of rbc that mature

A

Reticulocyte

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2
Q

Retics mature for how many days

A

2-4 days

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3
Q

It has a reticulum network which are rna and protoporphyrin remnants

A

Retics

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4
Q

Nv of retics in adults

A

0.5-1.5%, 20,000-60,000 mm3

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5
Q

Nv in newborn

A

2.5-6.5%

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6
Q

Physiologic increase of retics

A

Among new born
Menstruating women
Among oreg women

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7
Q

Abnormal increase retics

A

Pt with HA
PB poisoning
Pt with leukemia
Pt with malaria

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8
Q

Abnormally dec retics

A

Idiopathic aplastic anemia brought a dec retics

Acute benzol poisoning

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9
Q

Suppression of retics

A

Acute benzol poisoning

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10
Q

Under wet method

A

New mb
Cook, meyer, tureen method
Seiverd method

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11
Q

In wet method what are under BCB

A

Seiverds method

Cook, meyer, tureen mtd

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12
Q

Primary used in wet method

A

New methylene blue method

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13
Q

Dry method

A

Schilling rapid method
Osgood wilhery
Sabin method

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14
Q

Sabin method using

A

Janus green and neutral red

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15
Q

Osgood wilhelry using

A

Methylene blue

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16
Q

Schilling rapid method using

A

Brilliant cresyl blue

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17
Q

Nv of retics

A

20-60X10,4 mm3

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18
Q

ARC NV

A

24-84X10,9/L

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19
Q

Follows the loaw of osmosis

A

Erythrocyte osmotic fragility test

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20
Q

Checking the stability of rbc

A

Eoft

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21
Q

Isotonic solution

A

0.85-0.9% nacl

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22
Q

Hypertonic soln

A

> 0.85% nacl

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23
Q

Hypotonic soln

A
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24
Q

Shrink

A

Hypertonic

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25
Swell
Hypotonic
26
No changes in the morphology rbc
Isotonic
27
2 types of cell in EOFT
Sickle cell | Spherocyte
28
Fragile cell
Spherocyte
29
Resistant cell presence of HbS
Sickle cell
30
It test the stab of rbc ynder diff conc of hypotonic Nacl soln
Sanford method
31
Solution of sanford
0.50% nacl
32
Sanform preparation
12 test tube numbered witg 25-14
33
Specimen used in sanford
Fresh heparinuzed blood
34
Initial hemolysis normally in tube
16 or 17
35
Increase EOFT normally in tube
24
36
Complete eoft
19
37
Dec eoft initially
Tube 19
38
Tube with compact sediment and clear supernate
No hemolysis
39
1st from left that shows no sediments and with dark red supernate
Compelete hemolysis
40
1st tube from left not so compact sediment wupith pink supernate
Initial hemolysis
41
Dec eoft complete
15
42
Method under eoft
``` Sanford mtd Modified sanford Giffin and sanford Dacies mtd Fragiliograph mtd ```
43
Automated method of eoft
Fragiliograph mtd
44
Blood in diluted in hypotonic nacl soln buffer 7.4 oh added with po4
Dacies metd
45
Inc ofi
Hereditary spherocytosis
46
Dec ofi
Sickle cell SIDA Thalassemia Jaundice
47
In caise of hemolytic anemia
Hereditary soberocytosis
48
Refers to the number of eosino per cubic mm of blood
Esinophil ct
49
Nv of eosinophil
150-300mm3
50
Eosinophil detects
Parasitic, allergicm normal adrenal fxn
51
Mtd under eosinophil
Pilot method Friedman method Randolph mtd
52
A sample of blood is diluted with a solution that selectively stain eo and eliminates leukocytes from vie
Randolph
53
Rgts under eosinophil
``` Propylene glycol Dstilled water Naco3 Philoxine Philoxine ```
54
Diluting agent of eosino
Distilled water
55
Sed to hemolyse rna
Propylene glycol
56
Make other cell invisible and enhances eosinophil granules
Nac03
57
Stain of eosinophil
Philoxine
58
Used to detect normal adrenal fxn
Thorn test
59
Dec eosinoohil under cushing dse
Hyperaldrenalism
60
Inc eosino but there is an absence of eosino response under addisons dse
Hypoaldrenalism
61
Inject how many ACTH and how many hours
25 mg of ACTH and wait for 4 hours
62
How many hours of dfasting
12 hours
63
50% from base line eosino is
Normal
64
Value baseline 0-30 mm3 after 4 hours a pt has
Hyperaldrenalism
65
If pt value remain increase or no change the baseline it is
Hypoaldrenalism