Skin Biopsy Lab Flashcards Preview

Pathology > Skin Biopsy Lab > Flashcards

Flashcards in Skin Biopsy Lab Deck (15):
1

Why biopsy?

to make a DX of any swelling of tissue

2

4 reasons to biopsy..

1. confirm DX before administering potentially harmful drugs
2. determine cause of non-healing wounds
3. quickly determine etiology of aggressive or unusual skin lesions
4. determine cause of lesions that develop during the course of therapy

3

When should you biopsy?

When lesions are ACTIVE or BEFORE use of a therapy that might alter the histologic appearance of the lesions

4

What 2 types of drugs may alter the histologic appearance of skin lesions?

corticosteriods
immune-modulatory drugs
antimicrobials

5

It is best to biopsy the ___ spectrum of lesions present.

full

6

Where to biopsy?

multiple cutaneous sites that represent the full range of lesions (both 1ry & 2ry)

7

Be sure to label each sample with the _____ it was collected from.

location

8

How do you prepare a site for biopsy?

1. trim hair short (gently)
2. DO NOT surgically prepare the site (disinfect w/ 70% alcohol & let air dry)

9

Technique to biopsy lesions.

1. multiple samples representative of the range of lesions
2. properly label w/ location
3. always include crusts, if present

10

Technique for alopecic conditions.

1. collect sample from most alopecic area
2. draw line in direction of hair coat growth

11

Technique for ulcerative & de-pigmenting lesions.

collect sample from the junctional zone --> margin btwn normal & abnormal skin

12

Size of punch biopsy for haired skin?

6-8 mm punch

13

Size of punch biopsy for periocular skin, paw pads, or nasal planum?

4-6 mm punch

14

Technique for incisional biopsy.

1. take representative sample of the entire lesion + some normal skin
2. elliptical incision --> easier to stitch up

15

How should skin biopsies be fixed? (TQ)

in 10% buffered formalin immediately after collection
fix:tissue ratio= 10:1