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Histopath LEC > STAINS_COLOR > Flashcards

STAINS_COLOR Flashcards

1
Q

PAS

A

Magenta red

CHO, Glycogen, Mucins, Bacteria & Fungi, basement membrane

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2
Q

PAS w/ diastase

A

Red

Glycogen

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3
Q

Best carmine

A

Bright red

Glycogen

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4
Q

Langhan’s iodine

A

Mahogany brown

Glycogen

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5
Q

Alcian blue

A

Blue

Acid mucin

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6
Q

Alcian blue-PAS

A

Acid mucin: blue
Neutral mucin: magenta

Any mucins (acid/neutral)

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7
Q

Gomori’s aldehyde fuchsin stain

Sulfated mucins:
Carboxylated mucins:

A

purple
blue

Acid MPS Sulfated mucins Carboxylated mucins

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8
Q

Mucicarmine stain

A

Red

Cryptococcus neoformans Mucins

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9
Q

Colloidal iron

A

Dark blue

Acid mucin

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10
Q

Acridine orange

Acid mps
Fungi

A

Black
Greenish red fluorescence

Acid mucins/MPS Fungi

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11
Q

Sudan black

A

Blue black

Lipids

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12
Q

Sudan IV

A

Red

Lipids (TAG)

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13
Q

Sudan lll

A

Red

CNS

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14
Q

Oil red O

A

Brilliant red

Lipids

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15
Q

Osmium tetroxide

A

Black

Lipids

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16
Q

Nile blue sulfate method

Neutral fat
Cholesterin esters
Cholesterin fatty acids
Fatty acids & soap
Cerebrosides

A

= Pinkish red
= Light red
= Light red
= Deep blue to violet
= Light blue

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17
Q

Toluidine blue-acetone mtd

A

Metachromatic redbrown or yellow

Sulfatide

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18
Q

Borohydride-Periodic-Schiff method

A

Red

Gangliosides

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19
Q

Alkaline fast-green method

A

Green

Histones
Protamines

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20
Q

Peracetic acid-Alcian blue

A

Blue-green

Cystine
Cysteine

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21
Q

Sakaguchi

A

Orange-r

Arginine

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22
Q

Gomori calcium

A

Brownish black

Alkaline phosphatase

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23
Q

Gomori lead method

A

Black

Acid phosphatase

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24
Q

Lead method

A

Blackish brown

5’-nucleotidase

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25
Metal precipitation
Dark brownish-black ppt ATPase
26
Calcium cobalt method
Cobalt phosphate ppt ATPase
27
α-naphthyl acetate method
Reddish brown Non specific esterase
28
Indoxyl acetate method
Blue Nonspecific esterase
29
Tetrazolium method
Bluish black Monoamine oxidase
30
Feulgen technique
Red-purple DNA
31
Methyl green-pyronin
RNA (nucleoli): red DNA (chromatin): green
32
Gomori’s silver impregnation stain
Black Reticulin fibers
33
Van Gieson’s stain
Collagen = Pink/deep red Muscle, cytoplasm, RBC, fibrin = Yellow
34
Masson’s trichrome stain
Collagen & mucus = Blue Muscle, RBC & keratin = Red
35
Mallory’s aniline blue
Collagen fibers, cytoplasm, fibroglia fibrils, axon cylinders, neuroglia = Red Elastic fibers = Pale pink/yellow RBCs, myelin sheets = Yellow
36
Azocarmine
CT Glomerular basement membrane Amyloid & mucous colloid = Deep blue
37
Weigert’s
Elastic fibers Dark-blue/blue-black
38
Verhoeff’s
Elastic fibers Black
39
Taenzer-Unna-Orcein mtd
Elastic fibers Dark-brown
40
Krajian’s technique
Elastic fibers = Bright red Fibrin & CT = Dark blue RBC = Orange-yellow
41
Martius-Scarlet-Blue
RBCs = Yellow Muscle = Red Collagen = Blue Fibrin = Red
42
Mallory’s PTAH
Fibrin, muscle striations, neuroglia, amoeba = Dark blue RBCs = Blue Myelin = Lighter blue Collagen, osteoid, cartilage, elastic fibers = Deep brownish-red
43
Congo red
Amyloid Red
44
Methyl violet-crystal violet method
Amyloid Purplish red
45
Thioflavin-T fluorescent staining
Amyloid Yellow fluorescence
46
Modified Gomori’s Trichrome stain
Muscle fibers = Red Collagen = Green
47
Lissamine fast red
Muscles, RBC = Red Collagen = Yellow
48
Schmorl’s Picro-Thionin method
Lacunae & canaliculi = Dark brown-black Bone matrix = Yellow/brownish-yellow
49
Bielschowsky’s technique
Neurofibril, axons & dendrites = Black on a grayish BG Neuroglia & collagen = Lightly stained
50
Bodian’s stain
Nerve fibers & nerve endings
51
Sevier-Munger technique
Peripheral neuritis = Black Axons = Black Myelin sheath = Light brown Neuritic plaques & tangles = Black Argentaffin granules = Black
52
Toluidine blue
Nissl granules & nucleoli Deep blue
53
Polychrome methylene blue
Nissl granules & nucleoli Deep blue
54
Thionine
Nissl granuls & nucleoli Purple
55
Cresyl fast violet
Nissl substance = Purple-dark blue Neurons = Pale purple blue
56
Weigert-Pal technique
Myelin sheath Blue black
57
Luxol fast blue
Myelin Blue-green
58
Weil’s method
Myelin Black
59
Cajal’s gold sublimate method
Astrocytes Black on a light brownish BG
60
Perl’s Prussian blue
Hemosiderin Deep blue
61
Gomori’s Prussian blue
Iron pigments Bright blue
62
Turnbull’s blue
Ferrous iron (Hemosiderin) Blue
63
Benzidine-nitroprusside stain
Hemoglobin & oxidase granules Dark blue
64
Mod. Fouchet’s technique
Bile pigments Emerald to blue green
65
Gmelin technique
Bile & hematoidin Blue-purple then green
66
Stein’s iodine test
Bile pigments
67
Schmorl’s ferric ferricyanide method
Bile, lipofuscins, melanin, argentaffin cells, chromaffin, thyroid colloid Dark blue
68
Gomori’s aldehyde fuchsin
Lipofuscin Purple
69
Hemofuscin Red
Mallory’s fuchsin stain
70
Masson Fontana technique
Melanin = Black Argentaffin cell granules = Black
71
Von Kossa’s silver nitrate method
Calcium Black
72
Lindquist modified rhodanine technique
Copper Red to orange-red
73
Gram-Twort stain
Gram (+) organisms = Blue-black Gram (-) organisms = Pink-red RBCs = Green Elastic fibers = Black
74
Brown & Brenn method
Gram (+) bacteria = Blue Gram (-) bacteria = Red
75
Wade-Fite technique
M. leprae = Golden yellow
76
Toluidine blue
H. pylori = Dark blue against blue BG
77
Cresyl violet acetate mtd
H. pylori = Blue-violet
78
Dieterle method
L. pneumophila & spirochetes = Dark brown to black
79
Levaditi’s method
Spirochetes = Black on a yellowish BG
80
Modified Steiner & Steiner technique
Spirochetes, Donovan bodies, fungi, bacteria = Black
81
Warthin-Starry method
Spirochetes = Black
82
Grocott Methenamine Silver
Fungi = Sharply outlined I black Mucin & glycogen = Gray-black Mycelia & hyphae = Old rose RBCs = Yellow
83
Lendrum’s phloxinetartrazine method
Viral inclusions = Bright red
84
Orcein method
HBsAg = Brown-black
85
Giemsa stain
Bacteria = Blue Mast cell granules = Deep blue Eosinophilic granules = Red Nuclei = Blue Cytoplasm = Pink

Histopath LEC (37 decks)

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