Sterilization And Disinfection

Question 1

Used to decontaminate certain biological waste and sterilized media instruments and lab ware

Answer 1

Autoclave

Question 2

What are the different types of autoclave

Answer 2

1. Pressure cooker type
2. Horizontal autoclave
3. Vertical autoclave
4. Common laboratory autoclave
5. Large automatic hospital autoclave

Question 3

The first autoclave like pressurized vessels similar to what is known as pressure cooker

Answer 3

Denis papin

Question 4

When did denis papin was built

Answer 4

1679

Question 5

Work with louis pasteur to create first autoclave for medical and scientific purposes

Answer 5

Charles chamberland

Question 6

When did pasteur and chamberland build autoclave for Medical and scientific purposes

Answer 6

1879

Question 7

Refer to as a steam sterilizer

Answer 7

Sterilization

Question 8

What are the four aspect vital to effective sterilization

Answer 8

Time
Temperature
Pressure
Steam

Question 9

What is the standard time for autoclave

Answer 9

15 minutes

Question 10

What is the standard autoclave temperature

Answer 10

121°C (250°F)

Question 11

Does the boiling point of water kill endospores

Answer 11

No

Question 12

Is used as an indicator to confirm that successful sterilization cycle was completed

Answer 12

Geobacillus stearothermophilus

Question 13

What is the minimum pressure needed in autoclave

Answer 13

15 psi

Question 14

It provides insulation to pathogens from the sterilizing environment and are removed during pressure ramping so that pathogens do not escape proper sterilization

Answer 14

Air pockets

Question 15

Transfer of heat is mostly efficiently done using

Answer 15

Steam

Question 16

Refers to a liquid culture medium

Answer 16

Broth

Question 17

It indicates bacterial growth in a liquid state

Answer 17

Turbidity

Question 18

Broth are usually kept in a ____ and _____

Answer 18

Test tube and flask

Question 19

It is known as dehydrated culture media that used to grow and isolate microorganism

Answer 19

TSB (Tryptic Soy Broth)

Question 20

TSB served as the source of _____ and _____for the microorganism

Answer 20

Nitrogen and amino acid

Question 21

Refers to a solid culture medium used to grow microbes on a plate

Answer 21

Agar

Question 22

Agar is a ____ like substance derived from _____ that is used in food, medicine, and microbiology

Answer 22

Jelly, red algae

Question 23

What are the various types of agar

Answer 23

Nutrient agar
Blood agar
MacConkey agar
EMB agar
XLD agar

Question 24

A common medium for cultivating an isolating a wide range of microorganisms, it also supports the growth of most non fastidious bacteria

Answer 24

Nutrient agar

Question 25

Technique used to culture microorganisms on agar plate

Answer 25

Plating

Question 26

Technique used to estimate the number of microorganism in a sample with an unknown concentration

Answer 26

Serial dilution

Question 27

It involves diluting a sample multiple times to reduce the concentration to a countable level

Answer 27

Serial dilution

Question 28

What are the common plating techniques

Answer 28

Streak plate method Spread plate method Pour plate method

Question 29

A loop full of specimen is straight across an agar plate in an zinzag pattern

Answer 29

Streak plate method

Question 30

A liquid or suspension sample is spread across an agar plate

Answer 30

Spread plate method

Question 31

A liquid sample is mixed with molten agar and poured into a petri dish

Answer 31

Pour plate method

Question 32

A laboratory technique used to isolate pure cultures of bacteria from mixed population

Answer 32

Streak plate method

Question 33

Widely used culture techniques in microbiology laboratories due to its ease and simplicity

Answer 33

Spread plate method

Question 34

Sample of spread plate method must be in _____ or _____

Answer 34

Liquid or suspension

Question 35

A tool used to smoothly spread cells and bacteria on a culture plate

Answer 35

Plate spreader or cell spreader

Question 36

Suitable for facultative microerophilic and anaerobic microorganism

Answer 36

Pour plate method

Question 37

A laboratory instrument used to measure and transfer very small amounts of liquid

Answer 37

Micropipette

Question 38

A small disposable attachment used with a micropipette to accurately measure and transfer very small volume of liquid

Answer 38

Micropipette tips