Sterilization & Disinfection

Question 1

Ultrasonic cleaner

Answer 1

comes from washer sterilizer

removes small particles from areas mechanical or manual means can’t reach

cavitation

Question 2

Items for ultrasonic cleaning

Answer 2

box locks, serrations, ratchets

Question 3

Cavitation

Answer 3

high freq. sound waves –> vibration

bubbles implode –> vacuum that dislodges particles of bioburden

Question 4

Product associated parameters (5)

Answer 4

bioburden

bioresistance

biostate

bioshielding

density

Question 5

Endoscope soaked in disinfectant solution should…

Answer 5

be rinsed thoroughly w/ sterile water before use

Question 6

5 factors of steam sterilization

Answer 6

time in contact

contact

temp

moisture

pressure

Question 7

EO

Answer 7

kills by disruption metabolism of protein

organic ether gas

plastic, rubber, heat/moisture sensitive items

bacillus subtillis

Question 8

Why double wrap?

Answer 8

microbial barrier

Question 9

Prior to opening packs always…

Answer 9

inspect for integrity

Question 10

Instruments w/ lumens

Answer 10

frazier suction/rubber tubing

special prep

Question 11

Trapped air in lumen

Answer 11

prevents steam from getting inside

leave distilled water inside

Question 12

Basins __ if nested

Answer 12

separated by a porous material

Question 13

Linen packs shouldn’t exceed __ lbs

Answer 13

12lbs

Question 14

Chemical indicators

Answer 14

external tape, label, or paper

change color when exposed to physical conditions

doesn’t guarantee sterility

Question 15

Biological indicators

Answer 15

guarantee sterility

bacillus sterathermophilus - steam/implants

bacillus subtillis - dry heat, EO, peroxide

Question 16

Thermal methods

Answer 16

physical - steam under pressure, hot air/dry heat

Question 17

Time + temp has guaranteed…

Answer 17

sterility

Question 18

Loading sterilizer

Answer 18

flat packs on edge

small packs criss-crossed on each other

basins on sides

Question 19

Flash sterilization

Answer 19

sterilize unwrapped dropped instruments

only if needed immediately

Question 20

Min. lbs of pressure in steam sterilization cycle

Answer 20

27 psi

Question 21

Ionizing radiation

Answer 21

cobalt 60

irradiation for chem/thermal energy

death by DNA disruption

gamma rays/beta particles

Question 22

Disadvantages of hydrogen peroxide

Answer 22

metal trays block frequency

can’t use with cellulose

nylon becomes brittle

not for flexible endoscopes

Question 23

Event related sterility

Answer 23

replaced time system

contamination is event related not time related

Question 24

Asepsis

Answer 24

free of pathogenic organisms

Question 25

Bacteriostatic

Answer 25

inhibits bacterial growth

Question 26

Lumen

Answer 26

duct

Question 27

Permeability

Answer 27

rate of flow through porous material

Question 28

Steam + lumens

Answer 28

water will boil inside and displace air in lumen important in gravity displacement steam sterilization

Question 29

Basin sterilization specs

Answer 29

250F for 20min minimum

Question 30

Chemical methods

Answer 30

EO formaldehyde gas/solution peroxide plasma/vapor ozone gas acetic acid soln glutaraldehyde solution peracetic acid solution

Question 31

Rapid readout bio indicator

Answer 31

high speed pressure steam sterilization w/ gravity displacement cycle flurometric detection of B. stearothermophilis bound enzyme

Question 32

Flash sterilizer times

Answer 32

mixed load 10min @ 270F metal, non porous, no lumen 3min. @ 270F

Question 33

EO effectiveness

Answer 33

85-145F time gas concentration humidity

Question 34

Bioshielding

Answer 34

packing material characteristics

Question 35

Density

Answer 35

factors effecting penetration or evacuation of the agent

Question 36

Basins and condensate

Answer 36

attract it because of position and density in sterilizer

Question 37

Why don't you sterilize sponges in basins?

Answer 37

deflect steam preventing contact

Question 38

Max weight for wrapped basin set?

Answer 38

7lbs

Question 39

When woven wrapped items become unsterile?

Answer 39

unwrap and completely re-process/re-wash

Question 40

Muslin wrap max size of linen pack?

Answer 40

12x12x20

Question 41

Where do you place basins?

Answer 41

on their side in autoclave lowest shelf

Question 42

Sterilization

Answer 42

destruction of all microorganisms

Question 43

Methods of sterilization

Answer 43

steam chemical high velocity electron bombardment ultraviolet radiation

Question 44

Glutaraldehyde disinfection and sterilization times

Answer 44

20 min. @ rm temp to disinfect 10hrs. immersed to sterilize

Question 45

Chelation

Answer 45

binding minerals in a solution to prevent their deposit on the surface on instruments causes spotting

Question 46

Liquid chemical process

Answer 46

formaldehyde abandoned peracetic acid/steris skin tumors in mice submerge with all sides in fluid

Question 47

Biostate

Answer 47

nutritional, physical, reproductive phase

Question 48

After laundering, woven fabrics should...

Answer 48

store at room temp 64-72F at 35-70% humidity for 2 hrs to rehydrate

Question 49

Dry heat

Answer 49

used for oils, petroleum, talc steam cannot penetrate these physical oxidation/slow burning of protein higher temp needed because of less moisture

Question 50

Bioresistance

Answer 50

heat/reproductive phase

Question 51

Process associated parameters

Answer 51

temp humidity/moisture/hydration time purity of agent + air + residue saturation/penetration capacity of sterilizer/position of items

Question 52

Recommendations for prepping items for decontamination

Answer 52

perforated or wire mesh trays heavy on bottom hinged open attachments disassembled