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AUBF > Stras: Amniotic fluid > Flashcards

Stras: Amniotic fluid Flashcards

1
Q

Which of the following is not a function of amniotic fluid?
A. Allow movement of the fetus
B. Carbon dioxide and oxygen exchange
C. Protect fetus from extreme temperature changes
D. Protective cushion for the fetus

A

B. Carbon dioxide and oxygen exchange

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2
Q

What is the primary cause of the normal increase in amniotic fluid as a pregnancy progresses?
A. Fetal cell metabolism
B. Fetal swallowing
C. Fetal urine
D. Transfer of water across the placenta

A

C. Fetal urine

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3
Q

Which of the following is not a reason for decreased amounts of amniotic fluid?
A. Fetus fails to begin swallowing
B. Increased fetal swallowing
C. Membrane leakage
D. Urinary tract defects

A

A. Fetus fails to begin swallowing

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4
Q

Why might a creatinine level be requested on an amniotic fluid?
A. Detect oligohydramnios
B. Detect polyhydramnios
C. Differentiate amniotic fluid from maternal urine
D. Evaluate lung maturity

A

C. Differentiate amniotic fluid from maternal urine

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5
Q

Amniotic fluid specimens are placed in ambercolored tubes prior to sending them to the laboratory to prevent the destruction of:
A. Alpha fetoprotein
B. Bilirubin
C. Cells for cytogenetics
D. Lecithin

A

B. Bilirubin

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6
Q

How are specimens for FLM testing delivered to and stored in the laboratory?
A. Delivered on ice and refrigerated or frozen
B. Immediately centrifuged
C. Kept at room temperature
D. Delivered in a vacuum tube

A

A. Delivered on ice and refrigerated or frozen

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7
Q

Why are amniotic specimens for cytogenetic analysis incubated at 37C prior to analysis?
A. To detect the presence of meconium
B. To differentiate amniotic fluid from urine
C. To prevent photo-oxidation of bilirubin to biliverdin
D. To prolong fetal cell viability and integrity

A

D. To prolong fetal cell viability and integrity

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8
Q

Filtration of amniotic fluid is required to avoid decreased values in the test results for:
A. Bilirubin
B. Fetal cells
C. Phospholipids
D. Urea

A

C. Phospholipids

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9
Q

Match the following colors in amniotic fluid with their significance.

Colorless

A

Normal

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10
Q

Match the following colors in amniotic fluid with their significance.

Dark green

A

Presence of meconium

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11
Q

Match the following colors in amniotic fluid with their significance.

Red-brown

A

Fetal death

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12
Q

Match the following colors in amniotic fluid with their significance.

Yellow

A

Presence of bilirubin

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13
Q

A significant rise in the OD of amniotic fluid at 450 nm indicates the presence of which analyte?
A. Bilirubin
B. Lecithin
C. Oxyhemoglobin
D. Sphingomyelin

A

A. Bilirubin

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14
Q

Plotting the amniotic fluid OD on a Liley graph represents the severity of hemolytic disease of the newborn. A value that is plotted in zone II indicates what condition of the fetus?
A. No hemolysis
B. Mildly affected fetus
C. Moderately affected fetus that requires close monitoring
D. Severely affected fetus that requires intervention

A

C. Moderately affected fetus that requires close monitoring

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15
Q

The presence of a fetal neural tube disorder may be detected by:
A. Increased amniotic fluid bilirubin
B. Increased maternal serum alpha fetoprotein
C. Decreased amniotic fluid phosphatidyl glycerol
D. Decreased maternal serum acetycholinesterase

A

B. Increased maternal serum alpha fetoprotein

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16
Q

True or False: An AFP MoM value greater than twotimes the median value is considered an indication of a neural tube disorder

A

TRUE

17
Q

When severe HDN is present, which of the following tests on the amniotic fluid would the physician not order to determine whether the fetal lungs are mature enough to withstand a premature delivery?
A. AFP levels
B. Foam stability index
C. Lecithin/sphingomyelin ratio
D. Phosphatidyl glycerol detection

A

A. AFP levels

18
Q

The foam or shake test is a screening test for which amniotic fluid substance?
A. Bilirubin
B. Lecithin
C. Alpha fetoprotein
D. Creatinine

A

B. Lecithin

19
Q

True or False: Prior to 35 weeks’ gestation, the normal L/S ratio is less than 1.6.

A

TRUE

20
Q

When performing an L/S ratio by thin-layer chromatography, a mature fetal lung will show:
A. Sphingomyelin twice as concentrated as lecithin
B. No sphingomyelin
C. Lecithin twice as concentrated as sphingomyelin
D. Equal concentrations of lecithin and sphingomyelin

A

C. Lecithin twice as concentrated as sphingomyelin

21
Q

True or False: Phosphatidyl glycerol is present with an L/S ratio of 1.1.

A

TRUE

22
Q

A rapid test for FLM that does not require performance of thin-layer chromatography is:
A. AFP levels
B. Amniotic acetylcholinesterase
C. Aminostat-FLM
D. Bilirubin scan

A

C. Aminostat-FLM

23
Q

Does the failure to produce bubbles in the Foam Stability Index indicate increased or decreased lecithin?
A. Increased
B. Decreased

A

B. Decreased

24
Q

Microviscosity of amniotic fluid is measured by:
A. Thin-layer chromatography
B. Immunologic agglutination
C. Spectrophotometer
D. Fluorescence polarization

A

D. Fluorescence polarization

25
Q

The presence of phosphatidyl glycerol in amniotic fluid fetal lung maturity tests must be confirmed when:
A. Hemolytic disease of the newborn is present
B. The mother has maternal diabetes
C. Amniotic fluid is contaminated by hemoglobin
D. Neural tube disorder is suspected

A

B. The mother has maternal diabetes

26
Q

Immunologic agglutination test

A

Amniostat-FLM

27
Q

Uses albumin as the internal standard

A

Microviscosity Test

28
Q

Uses the platelet channel on a hematology instrument

A

Lamellar body count

29
Q

Uses sphingomyelin as an internal standard

A

L/S ratio

30
Q

True or False: An L/S ratio of 2.0 correlates with a surfactant/albumin ratio of 39 mg/g

A

FALSE

31
Q

A lamellar body count of 50,000 correlates with:
A. Absent phosphatidyl glycerol and TDx-FLM II ratio of 39
B. L/S ratio of 1.5 and absent phosphatidyl glycerol
C. OD at 650 nm of 1.010 and an L/S ratio of 1.1
D. OD at 650 nm of 0.150 and an L/S ratio of 2.0

A

D. OD at 650 nm of 0.150 and an L/S ratio of 2.0

32
Q

Which test for FLM is least affected by contamination with hemoglobin and meconium?
A. Amniostat-FLM
B. Foam Stability
C. Lamellar Bodies Count
D. TDx-FLM II

A

A. Amniostat-FLM

AUBF (38 decks)

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