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bloodbank > ABO Blood Group System > Flashcards

ABO Blood Group System Flashcards

1
Q

WHAT ISTHE SPECIMEN USED IN ABO FORWARD TYPING

A. PATIENT RBC
B. SERUM
C. PLASMA

A

ABO Forward Typing / Front/ Direct Typing

Specimen: Patient RBC

Reagent: uses commercial antisera, Anti –A (Blue color), Anti- B (Yellow color)

Use: Detection of ABO Antigens

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2
Q

WHAT IS THE USED OF ABO FORWARD TYPING

A. DETECTION OF ABO ANTIBODIES
B. DETECTION OF ABO ANTIGEN
C. NOTA
D. AOTA

A

ABO Forward Typing / Front/ Direct Typing

Specimen: Patient RBC

Reagent: uses commercial antisera, Anti –A (Blue color), Anti- B (Yellow color)

Use: Detection of ABO Antigens

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3
Q

WHAT IS THE REAGENT USED IN INDIRECT TYPING

A. WHOLE BLOOF
B. ANTI - A , ANTI - B
C. A CELLS , B CELLS

A

ABO Reverse Typing / Back / Indirect Typing /Serum Typing

Specimen: Serum/plasma

Reagent: A cells, B cells

Use: Detection of ABO antibodies

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4
Q

WHAT IS THE USED OF ABO REVERSE TYPING

A. DETECTION OF ABO ANTIBODIES
B. DETECTION OF ABO ANTIGEN
C. NOTA
D. AOTA

A

ABO Reverse Typing / Back / Indirect Typing /Serum Typing

Specimen: Serum/plasma

Reagent: A cells, B cells

Use: Detection of ABO antibodies

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5
Q

WHAT IS THE TEMPERATURE OF BLOOD TYPING REAGENT WHEN NOT USED

A. ROOM TEMPERATURE
B. REF TEMPERATURE
C. FROZEN TEMMPERATURE

A

BLOOD TYPING REAGENTS

WHEN USED ———– ROOM TEMPERATURE

WHEN NOT USED ————- REF TEMPERATURE

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6
Q

WHICH OF THE FOLLOWING PLASMA PRODUCTS IS IDEAL TO BE TRANSFUSED TO A BLOOD TYPE AB PATIENT

a. Plasma product from blood type A donor
b. Plasma product from blood type B donor
c. Plasma product from blood type O donor
d. nota

A

nota

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7
Q

centrifugation time for blood typing

a. 10 secs
b, 20 secs
c. 30 secs
d. 60 secs

A

20 secs

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8
Q

universal donor of whole blood or PRBCs

a. blood type O
b. blood type A
c. blood type AB
d. blood type B

A

B.T O
Universal donor of whole blood or packed RBC

B.T AB
Universal acceptor/recipient of whole blood or packed RBC

B.T AB
Universal donor of plasma products

B.T O
Universal acceptor/recipient of plasma products

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9
Q

Universal acceptor/recipient of plasma products

a. blood type O
b. blood type A
c. blood type AB
d. blood type B

A

B.T O
Universal donor of whole blood or packed RBC

B.T AB
Universal acceptor/recipient of whole blood or packed RBC

B.T AB
Universal donor of plasma products

B.T O
Universal acceptor/recipient of plasma products

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10
Q

Universal donor of plasma products

a. blood type O
b. blood type A
c. blood type AB
d. blood type B

A

B.T O
Universal donor of whole blood or packed RBC

B.T AB
Universal acceptor/recipient of whole blood or packed RBC

B.T AB
Universal donor of plasma products

B.T O
Universal acceptor/recipient of plasma products

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11
Q

which of the following is recessive

a. A gene
b. B gene
c. O gene

A

A and B genes
- DOMINANT

O gene

  • Amorph /silent
  • Recessive
  • No antigen is produced
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12
Q

what is the immunodominant sugar in B gene

a. L-fucose
b. N- acetyl-D- galactosamine
c. D-galactose
d. nota

A

B gene

GLYCOSYLTRANSFERASE
- D-GALACTOSYLTRANFERASE

IMMUNODOMINAT SUGAR
- D- GALACTOSE

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13
Q

what is the immunodominant sugar in A gene

a. L-fucose
b. N- acetyl-D- galactosamine
c. D-galactose
d. nota

A

GENE
- A

GLYCOSYLTRANSFERASE
- N - ACETYLGALACTOSYLTRANSFERASE

IMMUNODOMINAT SUGAR
- N-ACETYL-D-GALACTOSEAMINE

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14
Q

amount of H ( from greatest to lowest)

a. O > A1 > A1B > A2 > A2B > B
B. O > A1B > A1 > A2B > B > A2
C. O > A2 > B > A2B > A1 > A1B
D. O > A2B > B > A2 > A1B > A1

A

AMOUNT OF H ( FROM GREATEST TO LOWEST)

O > A2 > B > A2B > A1 > A1B

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15
Q

what is the origin of type 2 chain

a. plasma
b. erythrocyte precursors
c. serum

A

type 2
linkage = Beta 1, 4
origin = ERYTHROCYTE PRECURSORS
controlling genes H,A,B

type 1

linkage = Beta 1, 3
origin = plasma
controlling genes = H, A , B ,Se , Lewis

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16
Q

how many forms of H antigen have

a. 1
b. 2
c. 3
d. 4

A

There are four Forms of H antigen (H1, H2, H3, and H4)

H1 AND H2 = Unbranched straight chain

H3 AND H4 = Complex branched chain

17
Q

what subgroup a is No agglutination with anti-A and Anti-AB, secretors contain only H substance and no substance in saliva

a. Am
b. Ay
c. Ael
d. Aend

A

A3
Mixed field agglutination with anti-A and or anti-AB
Ax
Weak agglutination with anti-AB only
Aend
<10% red cell shows very weak mf agglutination
Am
No agglutination with anti-A and anti-AB, secretors demonstrate QUANTITIES of A substance in saliva

Ay
No agglutination with Anti-A and anti-AB, secretors contain SMALL AMOUNT of A substance in saliva

Ael
No agglutination with anti-A and Anti-AB, secretors contain only H substance and no substance in saliva

18
Q

what subgroup a is <10% red cell shows very weak mf agglutination

a. Am
b. Ay
c. Ael
d. Aend

A

A3
Mixed field agglutination with anti-A and or anti-AB
Ax
Weak agglutination with anti-AB only
Aend
<10% red cell shows very weak mf agglutination
Am
No agglutination with anti-A and anti-AB, secretors demonstrate QUANTITIES of A substance in saliva

Ay
No agglutination with Anti-A and anti-AB, secretors contain SMALL AMOUNT of A substance in saliva

Ael
No agglutination with anti-A and Anti-AB, secretors contain only H substance and no substance in saliva

19
Q

what subgroup a is No agglutination with Anti-A and anti-AB, secretors contain SMALL AMOUNT of A substance in saliva

a. Am
b. Ay
c. Ael
d. Aend

A

A3
Mixed field agglutination with anti-A and or anti-AB
Ax
Weak agglutination with anti-AB only
Aend
<10% red cell shows very weak mf agglutination
Am
No agglutination with anti-A and anti-AB, secretors demonstrate QUANTITIES of A substance in saliva

Ay
No agglutination with Anti-A and anti-AB, secretors contain SMALL AMOUNT of A substance in saliva

Ael
No agglutination with anti-A and Anti-AB, secretors contain only H substance and no substance in saliva

20
Q

what is the source of Anti - B lectin

a. dolichos biflorus
b. bandeiraea simplicifolia
c. ulex europeus
d. aota

A

Bandeiraea simplicifolia

  - source of Anti- B lectin
  - used to agglutinates B cells that have been pretreated with enzymes

Dolichos biflorus
- source of Anti- A1 lectin

Ulex europeus
- source of anti H lectin

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