pre transfusion testing

Question 1

Pretransfusion testing begins and ends with the proper _______ of the patient sample

a, identification

b. collection
c. nota
d. aota

Answer 1

answer: identification and collection

Pretransfusion testing begins and ends with the proper _______ of the patient sample

Question 2

greatest threat to safe transfusion therapy

a. identification
b. collection
c. wrong label
d. clerical error

Answer 2

clerical error

> A major cause of transfusion-associated fatalities is _____ resulting in incorrect ABO groupings and transfusion of ABO incompatible blood.

> Clerical error is the greatest threat to safe transfusion therapy.

> The most common cause of error is misidentification of the recipient

Question 3

A major cause of transfusion-associated fatalities is _____ resulting in incorrect ABO groupings and transfusion of ABO incompatible blood.

a. identification
b. collection
c. wrong label
d. clerical error

Answer 3

clerical error

> A major cause of transfusion-associated fatalities is _____ resulting in incorrect ABO groupings and transfusion of ABO incompatible blood.

> Clerical error is the greatest threat to safe transfusion therapy.

> The most common cause of error is misidentification of the recipient

Question 4

In extreme emergencies, when there is no time to obtain and test a pretransfusion sample, group __ can be used

a. o rh + packed cells
b. o rh - packed cells
c. ab rh + packed cells
d. a rh - packed cells

Answer 4

O Rh-negative packed cells

In extreme emergencies, when there is no time to obtain and test a pretransfusion sample, group __ can be used

Question 5

Both donor and recipient samples must be stored for a minimum of ___ following transfusion

a. 7 days
b. 14 days
c. 4 days
d. 3 days

Answer 5

7 days

Both donor and recipient samples must be stored for a minimum of ___ following transfusion

Question 6

most critical pre transfusion serologic test

a. identifying the patient
b. correct ABO grouping
c. clerical error
d. misidentification of the recipient

Answer 6

Determining the patient’s correct ABO group is the most critical pretransfusion serologic test

Question 7

It is a final check of ABO compatibility between donor and patient

Answer 7

crossmatching

Question 8

final step of pre transfusion compatibility testing

Answer 8

crossmatching

Question 9

which of these two is for major crossmatching

a. psdr
b. dspr

Answer 9

major crossmatching (PSDR)
  patient serum+ donor plasma

minor crossmatching (PRDS)
  patient red cells + donor serum

Question 10

what are the phases of crossmatching

a. immediate spin
b. antiglobulin phase
c. saline phase
d. incubation phase

Answer 10

phases of crossmatching

1. Immediate spin crossmatching / saline phase / initial phase
2. incubation phase
3. antiglobulin crossmatching phase

Question 11

which of the 3 phases in crossmatching should be performed in room temperature

a. immediate spin
b. incubation phase
c. antiglobulin phase

Answer 11

immediate spin/ saline phase / initial phase
- done at room temperature

incubation and antiglobulin phase
- done at 37’C

Question 12

which of the 3 phases in crossmatching use nss as enhancement media

a. immediate spin
b. incubation phase
c. antiglobulin phase

Answer 12

immediate spin
- uses NSS or 22% albumin

incubation phase
- uses LISS

antiglobulin phase
- uses albumin , liss , polyethylene glycol and polybrene

Question 13

Adding ____________ to the test system reportedly eliminates some of the false-positive reactions

a. liss
b. saline
c. polybrene
d. ethylenediaminetetracetic acid

Answer 13

Adding ethylenediaminetetraacetic acid to the test system reportedly eliminates some of the false-positive reactions

Question 14

significant antibodies that can be detected in incubation phase

a. IgG
b. IgM
c. IgD
d. IgE

Answer 14

B. Incubation Phase
• Done at 37’C incubation
• Usage of enhancement media such as: LISS -> 15 minutes (5 to 15minutes)
• Detection of clinically significant antibodies such as IgG

Question 15

An autocontrol, consisting of the_______, may be tested in parallel with the antiglobulin crossmatch test.

a. patient rbc, donor plasma
b. patients own cells and serum
c. donors red cells, patients serum

Answer 15

C.Antiglobulin Crossmatching Phase

• The antiglobulin crossmatch procedure begins in the same manner as the immediate spin crossmatch, continues to a 37°C incubation, and finishes with an antiglobulin test
• Done at 37’C incubation
• Several enhancement media may be applied to boost antigen-antibody reactions. These may include albumin, low ionic strength solution (LISS), polyethylene glycol, and polybrene
• An autocontrol, consisting of the patient’s own cells and serum, may be tested in parallel with the antiglobulin crossmatch test.