ABO Blood Group System II Flashcards
what is specimen used to detect secretor status
a. whole blood
b. plasma
c. urine
d. saliva
Detection Of Secretor Status
Specimen: saliva
Principle: Hemagglutination inhibition
(+) non agglutination
which of the following antigens is / are present in saliva of a group a secretor
a. A only
b. A and H
c. Aand O
d. H only
group A/ AA SeSe
= A and H
In people whose blood type are B and A may have Anti-A and anti –B correspondingly, predominantly are ____
a. IgA
b. IgM
c. IgG
d. IgD
> ABO antibodies may be IgG, IgM (Predominant), and IgA (Henry’s).
> In people whose blood type are B and A may have Anti-A and anti –B correspondingly, predominantly are IgM
> In patient with blood type O, Anti-A, Anti-B, Anti-AB may be seen and predominantly are IgG
which of the following is Bombay phenotype
a. Oh
b. hh
c. H
d. nota
Bombay Phenotype = Oh
Bombay genotype = hh
is Bombay individual inherit a H gene?
a. yes
b. no
Bombay individual
DO NOT inherit H gene
which of the following antibody is considered ALLOANTIBODY
a. Anti - A
b. Anti - B
c. Anti - D
d. AOTA
ans: ANTI -D
alloantibody
- bago gawin sa katawan need muna nang maexposed sa RBC na wala sayo
ABO antibodies
- They are naturally occurring antibodies because they are produced without any exposure to RBCs.
which of the following discrepancies is most common
a. Group 1
b. Group II
C. Group III
D. Group IV
Group I
- Weakly reacting or missing antibodies
- Most common
- discrepancies on reverse typing
which of the following discrepancies is on reverse typing
a. Group 1
b. Group II
C. Group III
D. Group IV
Group I
- Weakly reacting or missing antibodies
- Most common
- discrepancies on reverse typing
which of the following discrepancies is on forward typing
a. Group 1
b. Group II
C. Group III
D. Group IV
Group II
- Weakly reacting or missing antigens
- least common
- discrepancies on forward typing
which of the following discrepancies is on protein or plasma abnormalities
a. Group 1
b. Group II
C. Group III
D. Group IV
Group III
- Protein or plasma abnormalities
- discrepancies on both forward and reverse
which of the following discrepancies is caused by high level of fibrinogen
a. Group 1
b. Group II
C. Group III
D. Group IV
Group III
- Protein or plasma abnormalities
- discrepancies on both forward and reverse
causes:
✓ Wharton’s jelly in cord blood samples
✓ High level of globulin: MM, WM, plasma cell dyscracias
✓ High level of fibrinogen
✓ Plasma expanders such as Dextran and polyvinylpyrrolidone
✓ Rouleaux
what is the remedy for Wharton’s jelly
a. repeat test
b. treat the RBCs with acetic anyhydride reacetylates
c. wash cell for 6 -8x
d. , wash the patient rbc several times withsaline
✓ Wharton’s jelly = wash Cell 6 to 8x
✓ For rouleaux = washing the patient’s RBCs several times with saline
what is the remedy in group 4 discrepancy caused by unexpected non abo alloantibodies
a. repeat reverse typing using 3 samples (a1 , a2, b and o cells)and autologous samples( px serum + px rbcs)
b. wash the px cells 3x with saline then retype
c. rbc treated with 0.01 M dithiothreitol
d. wash cell for 6-8x
✓ Wharton’s jelly = wash Cell 6 to 8x
> Cold auto antibodies = perform DAT test,
= treat with 0.01 M dithiothreitol
> Unexpected ABO isoagglutinins
a. reverse typing can be repeated using at least three examples of A1, A2, B cells; O cells; and an autologous control (patient’s serum mixed with patient’s RBCs) b. The patient’s RBCs can be tested with Dolichos biflorus
Unexpected alloantibodies
for antibody against Acriflavine (yellow dye used in anti-B reagents)
-Washing the patient’s cells three times with saline and then retyping them should resolve this discrepancy.
