pre transfusion testing II Flashcards
which of the enhancement media works by reducing the zeta potential and dispersing the charges, thus allowing the RBCs to approach each other.
a. 22% albumin
b. low ionic strength slain 0.2% NaCl
c. polybrene
d. polyethylene glycol
22% albumin
- reduce the zeta potential
liss 0.2% NaCl
- increase the uptake of antibody
polyethylene glycol
- removes water
which of the enhancement media increases the uptake of antibody onto the RBC during the sensitization phase.
a. 22% albumin
b. low ionic strength slain 0.2% NaCl
c. polybrene
d. polyethylene glycol
22% albumin
- reduce the zeta potential
liss 0.2% NaCl
- increase the uptake of antibody
polyethylene glycol
- removes water
which of the enhancement media removes water, thereby concentrating any antibodies present
a. 22% albumin
b. low ionic strength slain 0.2% NaCl
c. polybrene
d. polyethylene glycol
22% albumin
- reduce the zeta potential
liss 0.2% NaCl
- increase the uptake of antibody
polyethylene glycol
- removes water
which potentiators increase tests sensitivity
a. ahg
b. 22% albumin
c. liss
d. peg
peg
action : increases test SENSITIVITY; aggreagates rbcs causing closer proximity to RBCs TO ONE ANOTHER; Assisting in antibody cross linking
Procedure: incubate at 37’C for 10-30 min
which potentiators causes rbcs to take up antibody morerapidly
a. ahg
b. 22% albumin
c. liss
d. peg
LISS
action : causes rbcs to take up antibody morerapidly
procdure: incubation at 37’C for 5-15 mins, cell washing before iat
The blood components should then be infused as quickly as tolerated, or at most within _______
a. 8 hours
b. 4 hours
c. 1 hour
d. 2 hours
> The blood components should then be infused as quickly as tolerated, or at most within 4 hours
> about 200ml blood/hr
how man % of albumin to used to dilute blood components
a. 5%
b. 3%
c. 0.2%
d. 10%
Intravenous fluid- only (0.9%) isotonic saline or 5% albumin should be used to dilute blood components
when to check blood warmers
a. daily
b. monthly
c. quaterly
d. every 4 horus
quaterly
- blood warmers
- cell washer
- centrifuge speed timer
when to check heating blocks
a. daily
b. monthly
c. quaterly
d. every 4 horus
Daily When in Use
Heating blocks, Water Baths,
Donor unit agitators, Scales, Balances,
Hemoglobinometer, Microhematocrit centrifuges,
Refrigerator and Freezers (continuous monitoring)
when to check centrifuge temperature
a. daily
b. monthly
c. quaterly
d. every 4 horus
Monthly Alarm activation (freezers and refrigerators), centrifuge temperature(refrigerated)
