2.16 Transcription Flashcards Preview

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Flashcards in 2.16 Transcription Deck (15):
0

What is the Promoter?

Responsible for Binding RNA Polymerase II

1

What is the Cap Sequence

WHere transcription is initiated

2

What is the actual nucleotide sequence of the Cap Sequence?

ACATTTG

3

What is the ATG codon responsible for ?

Codon responsible for initiation

4

What is the TAA codon responsible for?

Termination codon

5

Where are CAAT and TATA boxes found and what is their role?

Found in the promoter sequence and function to attract polymerase and begin transcription

6

What are C/EBPs?

transcription factors that recruit other transcription factors and open chromatin

7

How does the enhancer function?

It can physically interact with the promoter via transcription factor interactions to modulate gene expressions

8

What are the three steps of Transcription

Initiation
Elongation
Termination

9

What occurs during the initiation phase?

RNA Polymerase II binds to promoter
This requires Transcription factors

10

What occurs during the Elongation phase

Synthesized 5' --> 3'
Antiparallel to DNA sequence
T's are replaced with U's

11

What is released during elongation each time a NT is added

Pyrophosphates

12

What occurs during Termination phase

5' cap is applied which is methylated guanosine
3' end receives a poly A tail

13

What are the two post-transcriptional modifications that concern the cap and tail?

5' cap of methylated guanosine is installed in 5' to 5' polarity bound by three phosphates
3' terminus is modified by having roughly 200 A residues added. This gets shorter as mRNA ages

14

What is the function of the spliceosome

Removal of introns via SnRNA