2nd sem: Intro to molecular medicine

Question 1

an rna blot for gene expression analysis

Answer 1

northern blot

Question 2

is ddNTPs used in PCR

Answer 2

oh hell no

Question 3

which enzyme is used to identify transcriptionally active regions in the DNA

Answer 3

Dnase1

Question 4

what enzyme can destroy a phosphodiester bond

Answer 4

RNAsEH

Question 5

vectors can transfer gene by conjugation

Answer 5

plasmid

Question 6

this vector is use for the cloning of DNA fragments up to 5000,00 bp

Answer 6

YAC

Question 7

this technique can detect single nucleases change based on altered mobility of the single stands of DNA

Answer 7

PCR-SSCP

Question 8

a collection of cloned DNA fragments in which only exons are represented

Answer 8

cDNA libaray

Question 9

this involves the use of VNTRs to establish a unique pattern of DNA of fragments for an individual

Answer 9

DNA fingering

Question 10

which method introduces new DNA into germ cells by its injection into the somatic cells

Answer 10

transgenic therapy

Question 11

what is the underlying principle for DNA cloning?

Answer 11

replication

Question 12

what is the underlying principle for DNA chips/microarray technology

Answer 12

complementary base pairing

Question 13

enzyme not required for the synthesis of cDNA

Answer 13

Dnase 1

Question 14

in the sanger method of DNA sequencing, what causes the termination of chain elongation

Answer 14

the incorporation dideoxynucleotide

Question 15

a plasmid is a

Answer 15

double stranded circular DNA

Question 16

fragments of DNA can be identified by the technique of

Answer 16

southern bolt

Question 17

a radioactive isotope labeled cDNA probe is used in

Answer 17

southern blotting and northern blotting

Question 18

optimum temperature of DNA polymerase of T.aquaticus is

Answer 18

72C

Question 19

the rate of migration of DNA within an agarose gel in the gel electrophoresis technique is primarily based on what factor

Answer 19

the size of the DNA fragments

Question 20

in the insertion of a cloning vector into a cloning host, why are the host cells plated on selective medium, which usually contains an antibiotic?

Answer 20

because only those host cells that have been transformed by the vector will grow in the presence of the antibiotic

Question 21

southern blot is used to

Answer 21

identify a specific DNA

Question 22

application of southern blotting includes

Answer 22

DNA fingerprinting

Question 23

northern hybridization is used to identify

Answer 23

specific RNA

Question 24

the separation technique of charged molecules under the influence of electric current is called

Answer 24

gel electrophoresis

Question 25

what type of probe would you use for western blot experiment

Answer 25

an antibody

Question 26

the first step in PCR or polymerase chain reaction is

Answer 26

denaturation

Question 27

in polymerase chain reaction, synthetic sequence of nucleotides are involved in

Answer 27

priming

Question 28

sequence recognized and cut by a restriction endonuclease

Answer 28

GGATCC

Question 29

when sticky ends are paired they can be joined by

Answer 29

DNA ligase

Question 30

generating DNA fragments to produce a library of DNA fragments results from the use of

Answer 30

restriction endonucleases

Question 31

a procedure called PCR is used to

Answer 31

copy gene sequences

Question 32

DNA fragments and DNA probes are to southern blotting as ____ are to northern blotting

Answer 32

RNA fragments and DNA probes

Question 33

why are heat stable DNA polymerases from thermophillic bacteria required for the polymerase chain reaction

Answer 33

these enzymes are stable enough to withstand temperatures required to melt DNA

Question 34

in gene therapy the desired gene is introduced into a__________ using a viral vector

Answer 34

somatic cell

Question 35

the major enzymes used in DNA recombinant technology are

Answer 35

restriction enzyme and DNA ligase