energy metabolism Flashcards by Bea Macatangay

this enzyme protects aerobic organisms from the product formed when one electron is transferred to a molecule of oxygen

superoxide dismutase

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all of the following are true regarding cytochrome P450, EXCEPT

a nonheme protein

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in the reaction which is the reductant

Fe2+ - Cu2+ –> Fe3+ - Cu1+

Fe2+ (Ferrous)

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In the krebs cycle,
malate is oxidized 🡆oxaloacetate, wherein the electron carrier goes to complex 1 of the respiratory chain. the reaction is catalyzed by what oxidoreductase

pyridine-linked dehydrogenase (PYR-LD)

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synthesis of ATP 🡰 ADP and inorganic phosphate by the free energy provided through hydrolysis of creatinine phosphate is an example of

substrate level phosphorylation

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which of the following enzymes is inhibited by oligomycin?

ATP synthase

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this subunit serves as proton channel in ATP synthase

F0 ATPase complex

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which of these inhibitors uncouples the respiratory chain and oxidative phosphorylation

2,4 dinitophenol and pentachlorophenol

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component of the respiratory chain which is NOT a proton pump

complex II

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which of these protein complexes of the ETC is inhibited by malonate?

succinate- Q reductase

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Standard G when ATP is hydrolyzed to ADP and Pi

-7.3 Kcal

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which of the ff limit the rate of respiration at state 2

availability of substrate only

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2nd law of thermodynamics states

the total entrophy of a system must increase if a process is to occur spontaneously

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which of the ff limit rate of respiration ate state 4

availability of ADP only

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which is not a site of energy conservation

complex II

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the energy that phosphorylates ADP to ATP in complex V is called

Proton motive force

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NADH dehydrogynase transfers electron to

FMN (flavin mononucleotide)

Vitamin B7

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which ROS is acted upon by dismutase

superoxide anion

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which of the ff enzymes is inhibited by malonic acid

NADH dehydrogenase

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which protein complex is inhibited by amytal

complex I
(amytal & rotenin =com I)
(malonate=com II)
(antimycin A= com III)
(cyanide, hydrogen sulfide and carbon monoxide = com IV)

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in what subunit of ATP synthase is ATP synthesized

BETA

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in this reaction, which is the oxidant

Fe2+ - Cu2+ –> Fe3+ - Cu1+

Cu2+ (cupric ion)

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how would you interpret a H that is negative

reaction releases heat
-H=exo= release heat
+H=endo=absorbed heat

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P:O ratio if FADH2 is the electron carrier in the ETC

1.5

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which protein complex is not a proton pump

succinate - Q reductase (complex IV)

cytochrome that transfer electrons directly to oxygen

Cytochrome a3

which of the ff protects the RBC from oxidative stress due to H202

glutathione peroxidase

what kind of oxidoreductase is cytochrome P450

Monooxygenase

what will be produced if molecular oxygen is reduced by 2 electrons

hydrogen peroxide

which inhibitor removes the link between ETC and oxidative phosphorylation?

dinitrophenol & pentachlorophenol (DTP, PCP)

what do you call the energy that is used to phosphorylate ADP to form ATP

proton-motive force

cyanide inhibits cytochrome oxidase binding to

cuprous Cu+= reducing agent (Cu2O)

what is the principal high energy intermediate or carrier compound

ATP

when H is Zero, the reaction is

isothermic

all of the following about the first law of thermodynamics are true, except

the total entrophy of a system must increase if a process is to occur spontaneously

which of the following thermodynamic concepts is correct

S is the degree of disorder

which of the ff is true if the G is negative

exergonic

amount of energy that is released from hydrolysis of ATP to ADP and Pi

-7.3 kcal/mol

the collecting point of electrons obtained from reduced NAD and FAD is

ubiquinone

what will be the consequence just in case NADP is not reduced to NADPH

decrease in the biosynthetic pathway at the level of reducatse

which if the ff enzyme incorporates an atom of oxygen 🡲 substrate 🡲 forming a hydroxylated product

monooxygenase

True about the 3rd law of thermodynamics

state known as "absolute zero" w/c is the bottom point on the kelvin temp scale

hydrolysis of ATP to ADP and Pi by the action of myosin ATPase releases energy which powered muscle contraction. this is made possible because according to the first law of thermodynamics

energy may be transformed into another form of energy

which of the following true if AG is positive

reaction is not spontaneous

how much energy is released when the beta high energy phosphate bond of ATP is cleaved to form AMP+PPi

G=-7.7 kcal/mol

a patient diagnosed with G-6PD deficiency is in danger of having hemolytic anemia because of increased levels of oxidative stress in his erythrocytes. normally the RBC has this enzyme which decomposes this toxic substance to water and oxygen

peroxidase

metabolism of lipophilic drugs is catalyzed by CP450 through hydroxylation which renders them water-soluble by

incorporating an atom of oxygen

one of the following oxidoreductase catalyzes the removal of hydrogen from a reduced substrate using NAD or FAD as intermediate electron acceptor

dehydrogenase

it uses 2 hydrogen peroxide as electron donor and electron acceptor

catalase

Cytochrome c links what protein complexes

complexes I and II to complex III

Synthesis of ATP through direct transfer of phosphate group from a high-energy substrate to ADP is called

substrate level phosphorylation

In the reaction: NADPH + GSSG → NADP + GSH. | Which is an oxidizing agent?

GSSG (glutathione disulfide)

Which of the following directly transfers electrons to oxygen?

Cytochrome a3

Which of the following removes the link between | the respiratory chain and oxidative phosphorylation?

2, 4-dinitrophenol

Which of the following protein complexes of the ETC produces one molecule of water?

Complex IV

The type of oxidoreductase that protects the body against damage by superoxide anion

dismutase

Transfer of 4 electrons to a molecule of oxygen results in the formation of

2 water molecules

Which of these conditions limit the rate of | respiration at state 4?

availability of ADP only

There is no translocation of proton in this protein complex of the ETC.

Complex II

The energy that phosphorylate ADP to ATP in | Complex V is called

proton motive force

In the respiratory chain, electrons from complexes I and II are transferred to

ubiquinone

NADH dehydrogenase uses this prosthetic group as electron acceptor in complex I

FMN

Transfer of one electron to one O2 molecule will produce

superoxide anion

Which protein complex of the respiratory chain is inhibited by malonate?

complex II

Which protein complex is inhibited by amytal?

complex I

What is the P:O ratio for FADH?

1.5

In what subunit of ATP synthase is ATP synthesized?

beta

energy metabolism Flashcards

(67 cards)