3-1 Flashcards Preview

Block 1 > 3-1 > Flashcards

Flashcards in 3-1 Deck (38):
1

what is job of condenser?

what is job of objective?

focus light on specimen

focus light FROM specimen

2

LM magnifies up to ?
EM magnifies up to ?

2500

500K

3

bright field microscopy shows little detail unless

specimen is stained with light absorbing dyes

4

"" microscopy gives a 3-D appearance to cell

No mar ski

5

fluorescent molecules do what

absorb light at one wavelength and emit photon at dif wavelength

6

describe the steps of cell fixation

-dehydrated in ethanol
-embedded in wax

7

describe TEM
-acronym
-magnification limit
-req of section

transmission electron microscopy

allows high magnification (up to 500K x)

needs dead, ultrathin sections

8

if you have high atomic #, you "" electrons (verb)

deflect

9

membranes attach to ""

cytoskeleton

10

define amphi-pathic

hydrophilic and hydrophobic

11

"fluid" in fluid mosaic model refers to

molecules diffuse laterally

12

steps of freeze fracture

1) frozen cells are fractured under vacuum
2) surface is coated with heavy metal AND cell is dissolved in acid...leaving a metal replica of SURFACE
3) surface is examined

13

P face is adjacent to ""

protoplasm

14

glycocalyx located in (internal/external) surface

glycocalyx consists of what molecules

purpose of glycocalyx

external

oligosaccharides attached to proteins and lipids

-protects cell surface against damage
-cell to cell recognition

15

Carriers in mitochondria import "" and export "".

Carriers in mitochondria import pyruvate and export ATP.

16

which cells use all 3 types of carriers (uniport, symport, antiport)

these are maintained in separate domains by ""

gut epithelial cells

tight junctions

17

cystic fibrosis is caused by

mutation in CF transmembrane conductance regulator

18

CFTR forms a ""

Cl channel

19

in absence of functioning CFTR...

not enough water reaches EC -> sticky mucus on outside of cell -> bad mucus clearance in lungs

20

ECM is produced by ""

rough ER and Golgi

21

fibrous proteins provide what

tensile strength (resist stretching)

22

fibrous proteins include what things

collagen

laminin (epithelia to basal lamina)

23

"" is the most abundant animal protein

collagen

24

carriers at basolateral surfaces mediate "" transport of glucose

passive

25

junctional complex is a term for

tight ju
adher ju
desmosomes

26

cadherin function

join together
-desmosome
-adherens

27

Desmosome function

bind cells together (esp much mechanical stress)

28

gap in gap junction exists due to "" spanning space bt cells

connexon

29

increase in "" causes changes that close the gap junction

Ca

30

dd

dd

31

the four techniques (Nomarski, phase contrast, etc) are used to examine what kinds of cells

Living, unstained

32

4 methods of using microscope

bright (field)
phase contrast
(Nomarski) phase contrast
dark (field)

33

TEM specimens MUST be stained with

heavy metal atoms

34

negative staining is

object is unstained
surrounding is stained

35

the mosaic aspect of the model was seen how

the fluid aspect was seen how

freeze fractured membrane surface

fuse together a mouse and human cell

36

"" or "" will close the gap junction

high Ca
low pH

37

dif bt tight and gap junction

tight = nothing passes thru them (seal)

gap = channels allow molecules to pass from cell to c

38

def of mindfulness

paying attention on purpose in present moment (non-judgmentally)