Flashcards in 3-1 Deck (38):
what is job of condenser?
what is job of objective?
focus light on specimen
focus light FROM specimen
LM magnifies up to ?
EM magnifies up to ?
bright field microscopy shows little detail unless
specimen is stained with light absorbing dyes
"" microscopy gives a 3-D appearance to cell
No mar ski
fluorescent molecules do what
absorb light at one wavelength and emit photon at dif wavelength
describe the steps of cell fixation
-dehydrated in ethanol
-embedded in wax
-req of section
transmission electron microscopy
allows high magnification (up to 500K x)
needs dead, ultrathin sections
if you have high atomic #, you "" electrons (verb)
membranes attach to ""
hydrophilic and hydrophobic
"fluid" in fluid mosaic model refers to
molecules diffuse laterally
steps of freeze fracture
1) frozen cells are fractured under vacuum
2) surface is coated with heavy metal AND cell is dissolved in acid...leaving a metal replica of SURFACE
3) surface is examined
P face is adjacent to ""
glycocalyx located in (internal/external) surface
glycocalyx consists of what molecules
purpose of glycocalyx
oligosaccharides attached to proteins and lipids
-protects cell surface against damage
-cell to cell recognition
Carriers in mitochondria import "" and export "".
Carriers in mitochondria import pyruvate and export ATP.
which cells use all 3 types of carriers (uniport, symport, antiport)
these are maintained in separate domains by ""
gut epithelial cells
cystic fibrosis is caused by
mutation in CF transmembrane conductance regulator
CFTR forms a ""
in absence of functioning CFTR...
not enough water reaches EC -> sticky mucus on outside of cell -> bad mucus clearance in lungs
ECM is produced by ""
rough ER and Golgi
fibrous proteins provide what
tensile strength (resist stretching)
fibrous proteins include what things
laminin (epithelia to basal lamina)
"" is the most abundant animal protein
carriers at basolateral surfaces mediate "" transport of glucose
junctional complex is a term for
bind cells together (esp much mechanical stress)
gap in gap junction exists due to "" spanning space bt cells
increase in "" causes changes that close the gap junction
the four techniques (Nomarski, phase contrast, etc) are used to examine what kinds of cells
4 methods of using microscope
(Nomarski) phase contrast
TEM specimens MUST be stained with
heavy metal atoms
negative staining is
object is unstained
surrounding is stained
the mosaic aspect of the model was seen how
the fluid aspect was seen how
freeze fractured membrane surface
fuse together a mouse and human cell
"" or "" will close the gap junction
dif bt tight and gap junction
tight = nothing passes thru them (seal)
gap = channels allow molecules to pass from cell to c