(3) Superficial, Cutaneous, & Subcutaneous Fungi

Question 1

Key Concepts of Superficial and Cutaneous Fungi

TOF

Superficial and cutaneous mycoses are among the most common of all non-communicable diseases

Answer 1

F

COMMUNICABLE

Question 2

Key Concepts of Superficial and Cutaneous Fungi

Most superficial and cutaneous fungal infections are caused by species of?

Answer 2

• Malassezia
• Dermatophytes
• Candida

Question 3

Key Concepts of Superficial and Cutaneous Fungi

TOF

The growth of dermatophytes is excited by serum and body temperature, and these fungi
rarely become invasive

Answer 3

F

INHIBITED not Excited

Question 4

Key Concepts of Superficial and Cutaneous Fungi

TOF

Geophilic and zoophilic dermatophytes usually cause acute, inflammatory lesions that respond
to topical treatment within weeks and rarely recur

Answer 4

True

Question 5

Key Concepts of Superficial and Cutaneous Fungi

TOF

aAnthropophilic dermatophytes usually cause relatively mild, chronic lesions that may require
months or years of treatment and frequently recur.

Answer 5

F

Anthrophilic

Question 6

this fungi has deeper site of infectivity

Answer 6

Subcutaneous fungi

Question 7

give the characterisitc of Subcutaneous fungi

Answer 7

• acquired through Skin Trauma or Skin prick
• The genus and species inhabit the Soil -habitat
• Biopsy to observe granules

Question 8

Give the genus and spp under the subcutaneous fungi

but not really genus and spp

Answer 8

1. Spotohrix schenckii
2. Madura foot agents
3. Chromoblastomycosis agent
4. Rhinosporidium seeberi
5. Loboa loboi
6. Subcutaneous zygomycosis

Question 9

Subcutaneous Fungi

this fungi invades the tissue and when seen under the microscope it has a cigar shaped bodies (asteroid body) - Yeast form

Answer 9

Spotohrix schenckii

Question 10

Subcutaneous Fungi

It is dimorphic in form and when In mold (the form) it has a flowerette conidia

Answer 10

Spothorix schenckii

Question 11

Subcutaneous Fungi

what form does the spothorix schenckii on the ff characteristics

• In RT
• Flowerette conidia or DAISY LIKE
• Infectious form (when enters the body)
• When in the body it can transform into a yeast form

Answer 11

Mold Form

Question 12

what form does the spothorix schenckii on the ff characteristics

• Cigar shaped
• Tissue Form

Answer 12

Yeast Form

Question 13

Subcutaneous Fungi

This is a fungi that is associated with a disease called Rose Gardener’s Disease, common in gardeners

Answer 13

Sporothrix Schenckii

Question 14

Sporothrix Schenckii infection are described as cord like multiple subcutaneous nodules

Answer 14

Rose gardener’s disease

Question 15

Subcutaneous Fungi

In lab diagnosis of Sporothrix Schenckii what is the growth on the culture (describe the colony)

Answer 15

white, cream, black, moist colonies

Question 16

Subcutaneous Fungi

This organism infect the FOOT

Answer 16

Madura Foot Agents

Question 17

Identify the Madura Foot Agent

Actinomycetes (Nocardia, Streptomyces) can cause similar infection: CAUSATIVE AGENT OF MADURA INFCETION

Answer 17

Bacterial

Question 18

Identify the Madura Foot Agent

• Pseudallescheria boydii (Most common cause)
• Madurella, Leptosphaeria, etc
• stained with PAS or H and E stain, granules can be seen

Answer 18

Fungal

Question 19

Madura Foot Agents

Once it infects the body, the
yeast or tissue form produces
____ inside the infectivity

this is also shown when the sample is stained and for IDENTIFICATIOn

Answer 19

Granules

Question 20

Madura Foot Agents

what is the lesion call for madara foot agent

note that fluid are secreted on these tracts

Answer 20

Granulomatous lesions on foot with multiple draining sinus tracts

Question 21

Madura Foot Agents

what lab specimen is used for its lab diagnosis and how is it processeds

Answer 21

1. Collect granules from sinuses, place sterile gauze overnight.
2. Culture granules to grow etiologic agents.
3. Tissue biopsy; H & E staining

Question 22

Madura Foot Agents

Match the FF

1. Black Grains
2. White Grains
3. Red Grains

a. Streptomyces somaliensis
b. Madurella mycetomatis
c. Streptomyces pelletierii

Answer 22

1. b
2. a
3. c

backla

Question 23

Subcutaneous Agent

• Chromoblastomycoses
• Dematiaceous fungi (dark pigmented under microscope)

Answer 23

Chromatoblastomycosis Agent

Question 24

Subcutaneous Agent

what are the type of sporulation of Chromoblastomycose (it is to ID genus and spp)

Answer 24

1. Phialophora verrucosa (VASE; Flask Shape)
2. Fonsecae pedrosoi (SHORT CHAIN sporulation)
3. Cladosporium carrianii (LONG CHAIN sporulation)

Question 25

# Subcutaneous Fungi Chromoblastomycosis agents when infected a tissue, what can be seen when stained and under micrscope

Answer 25

Infected Tissue: **BROWN SCELROTIC BODIES**

Question 26

Chromoblastomycosis agents causes what lesion

Answer 26

Cauliflower like lesion

Question 27

Culture is used for lab diagnosis of chromoblastomycosis, describe the colony it produse

Answer 27

- Dark colonies w/ jet black reverse - Slow growing, suede-like, olive black color

Question 28

KOH of skin scrapings/ crusts of Chromolastomycosis agent shows what characteristic

Answer 28

- **Brown planate** dividing rounded sclerotic bodies - Also as **copper pennies**, brown fission bodies

Question 29

Enumerate the types of conidation/sporulation (spp ID) for Chloroblastomyces

Answer 29

1. Phialophora 2. Cladosporium 3. Acrotheca

Question 30

# Subcutaenoues - Causing **RHINOSPORIDIOSIS** - Lesion: **polypoid masses** in the nose and pharynx producing nasal polyps

Answer 30

RHINOSPORIDIUM SEEBER

Question 31

what is the tissue form/yeast form of the rhinosporidum seeberi

Answer 31

**SPORANGIUM** - sac like structure filled with endospores, not culture | can be seen under the microscope

Question 32

# Subcutaneous Fungi - Producing a disease or infection SIMILAR TO DOLPHIN DSE - lesion: ** keloid-like subcutaneous nodule** usually involving the upper or lower extremities.

Answer 32

Loboa loboi

Question 33

describe the tissue form of the Loboa loboi under microscope

Answer 33

Multiple budding cells in chain

Question 34

- classification causing **Entomophthoromycosis** - Caused by **Conidiobolus coronatus-Rhinoentomophthoromycosis** - Caused by **Basidiobolus haptosporus-Subcutaneous Phycomycosis**

Answer 34

Subcutaneous Zygomycosis

Question 35

It is a member of subdivision ZYGOMYCOTA

Answer 35

- Non-septate Hyphae - Reproduce by zygospores (sexual)

Question 36

what is called the When you get infected, in the tissue by a Subcutaneous Zygomycosis fungi

Answer 36

Splendore-Hoeppli phenomenonon

Question 37

# Subcutaneous Zygomycosis fungi describe the Splendore-Hoeppli phenomenonon in tissue

Answer 37

Pink proteinaceous coat, around hyphae | can be seen when you stain

Question 38

# Subcutaneous Zygomycosis fungi other name of Entomophthromycosis

Answer 38

Conidiobolus | This name is more specific for the disease

Question 39

# Subcutaneous Zygomycosis fungi * Chronic inflammatory or granulomatous disease restricted to the nasal submucosa. * Characterized by polyps or palpable subcutaneous masses.

Answer 39

Conidiobolus

Question 40

TOF Subcutaneous Zygomycosis fungi is marked by swelling of the nose and perinasal tissued extending to the anal refion

Answer 40

F extending to the **periorbital region** | bobo pano umabot ng anal region

Question 41

Subcutaneous Zygomycosis fungi etiologic agent

Answer 41

**Conidiobolus coronatus** - Present in soil & decaying leaves

Question 42

# Subcutaneous Zygomycosis fungi enumerATE the lab diagnosis

Answer 42

* H & E stain of tissue biopsy * Culture on SDA then microscopic

Question 43

# Subcutaneous Zygomycosis fungi what is the observation under the H&E stain of tissue biopsy

Answer 43

**Broad sparsely septate hyphae** surrounded by eosinophilic sheath

Question 44

# Subcutaneous Zygomycosis fungi Characteristic of the CULTURE ON SDA

Answer 44

Flat, cream colored, glabrous, radially folded colony covered by fine, powdery, white surface mycelium.

Question 45

# Subcutaneous Zygomycosis fungi Describe the morphology under microscope

Answer 45

**Spherical sporangiola** (conidia) with **hair-like appendages** (villae)

Question 46

# LABORATORY DIAGNOSIS OF SUBCUTANEOUS FUNGI IN GENERAL: specimen submitted and how it can be processed

Answer 46

**Pus** (specimen) - Exudate - Biopsy - KOH - Culture - Serology, - Histologic stain

Question 47

TREATMENT OF SUBCUTANEOUS MYCOSES enumerate

Answer 47

* Antifungal agent **Amphotericin B** * **Surgical** removal of infected tissue, amputation (kapag Malaki na)

Question 48

# KEY CONCEPTS: SUBCUTANEOUS MYCOSES TOF Subcutaneous mycoses may be caused by dozens of **environmental molds** associated with **vegetation** and **soil**

Answer 48

TRUE

Question 49

# KEY CONCEPTS: SUBCUTANEOUS MYCOSES These infections are usually acquired when?

Answer 49

**minor cuts ** or **scratches** introduce soil or plant debris (eg, splinters, thorns) containing the pathogenic fungus

Question 50

# KEY CONCEPTS: SUBCUTANEOUS MYCOSES TOF The ensuing infections are frequently ACute but rarely spread to deeper tissues

Answer 50

F CHRONIC

Question 51

# KEY CONCEPTS: SUBCUTANEOUS MYCOSES S schenckii, the cause of sporotrichosis, is a?

Answer 51

**DIMORPHIC FUNGUS** converts from hyphal growth to yeast cells within the host

Question 52

# KEY CONCEPTS: SUBCUTANEOUS MYCOSES The diagnostic feature of chromoblastomycosis is the microscopic observation of?

Answer 52

*brownish (melanized)*, **spherical sclerotic bodies** within the lesions

Question 53

# KEY CONCEPTS: SUBCUTANEOUS MYCOSES The diagnostic feature of phaeohyphomycosis is the presence of?

Answer 53

*brownish (melanized),* **septate hyphae** within the lesions

Question 54

# KEY CONCEPTS: SUBCUTANEOUS MYCOSES The hallmark of a mycetoma is ____ and the formation of ____ that contain hard granules composed of hyphae and inflammatory tissue (eg, macrophages, fibrin)

Answer 54

- localized swelling - Fistulate

Question 55

# KEY CONCEPTS: SUBCUTANEOUS MYCOSES The hallmark of a mycetoma is localized swelling and the formation of fistulae that contain hard granules composed of?

Answer 55

hyphae and inflammatory tissue (eg, macrophages, fibrin)

Question 56

wow

Answer 56

ur done, jump