Parvovirus, Papovirus, Adenovirus Flashcards
(148 cards)
1
Q
Parvovirus, Papovirus, and Adenovirus are what type of virus?
A
DNA virus
2
Q
The only single-stranded DNA virus
A
Parvovirus
3
Q
Determine the virus:
- Single stranded
- Naked
- Icosahedral
- Linear
A
Parvovirus
4
Q
Determine the virus:
- Double-stranded
- Naked
- Icosahedral
A
Adenovirus and PaPoVavirus
PaPoVavirus ay circular
5
Q
Parvovirus
Discovered by and what year?
A
Yvonne Cossart in 1970s
6
Q
Parvovirus
Comes from the Latin word “____” which means?
A
“parvum” means small
7
Q
the smallest DNA animal virus
A
Parvovirus
8
Q
Parvovirus
Family:
Common name:
Virus:
A
- Parvoviridae
- Parvovirus
- Parvovrisu B-19
9
Q
Parvovirius: Characterisitc
The only known human parvovirus
A
Parvovirus B-19
10
Q
Parvovirus
What is the transmission
A
Close contact, probably respiratory
11
Q
Parvovirus
detection
A
- Serology
- polymerase chain reaction
(PCR) - Histology
12
Q
Parvovirus`
treatment
A
supportive
wow
13
Q
Parvovirus`
Prevention
A
Avoid contact
14
Q
Parvovirus: Viral Replication
- Virion ____ to the host cell
A
Attaches
15
Q
Parvovirus: Viral Replication
2.virion ____ and its DNA is ____
A
- Penetrates
- Uncoated
16
Q
Parvovirus: Viral Replication
3.Early transcription and translation of ____
A
mRNA
enzymes are synthesiez
17
Q
Parvovirus: Viral Replication
4.Late ____
5.Late ____
A
- Transcription (DNA is replicated)
- Translation (capsid proteins are synthesized)
18
Q
Parvovirus: Viral Replication
6.Virions ____
A
Mature
19
Q
Parvovirus: Viral Replication
7.Virions are ____
A
Released
for viral multiplication and infection
20
Q
what is the disease related to parvovirus?
A
ERYTHEMA INFECTIOSUM or FIFTH DISEASE
21
Q
ERYTHEMA INFECTIOSUM or FIFTH DISEASE
Diagnostic characteristic
A
“slapped” cheeks (blood vessels on cheeks would collpase
affects the endothelial cells
22
Q
ERYTHEMA INFECTIOSUM or FIFTH DISEASE
what does fifth disease causes?
A
- Aplastic Crisis
- Hydrops fetalis or hemolytic disease of newborns
23
Q
ERYTHEMA INFECTIOSUM or FIFTH DISEASE
systemic lupus erythematosus
A
Malar Rash
24
Q
ERYTHEMA INFECTIOSUM or FIFTH DISEASE
Blood picture would still exhibit normocytic and normochromic but RBC precursors will exhibit a sickle-shape which may develop sickle-cell
anemia or hemolytic anemia
A
Aplastic crisis
25
# ERYTHEMA INFECTIOSUM or FIFTH DISEASE
Aplastic Crisis:
Rbc will not reach the?
120 maturation days
26
# ERYTHEMA INFECTIOSUM or FIFTH DISEASE
50% of childbearing age are suisceptible of this disease and if it happend it causes miscarriages (during trimester)
Hydrops fetalis or hemolytic disease of newborns
27
# ERYTHEMA INFECTIOSUM or FIFTH DISEASE
mode of transmission?
- Repiratory routes (inhalation via droplets)
- Blood products
- Factor VIII and IX concentrates
- Transplacental (vertical - mother to baby)
28
# Parvovirus: Pathogenesis
If the virus enters through the respiratory tract, it may undergo 2 paths
- Local replication
- Replication in erythroid precursor cells in the bone marrow
29
# Parvovirus: Pathogenesis
local replication and replication in Erythroid precursor cells in BM leads to ?
Viremia
30
# Parvovirus: Pathogenesis
what is viremia?
Virus in the blood
31
# Parvovirus: Pathogenesis
Viremia →
| clue 2 paths
- VIral replication in upper repiratory tract
- Rash and arthralgia (muscle pain) (Erythemia infectiosum)
32
# Parvovirus: Pathogenesis
erythroid precursor
cells in the bone marrow →
| 3 paths
- Viremia (previous pathway)
- Normal host
- Host with chronic hemolytic anemia (or immunocompromised hosts)
33
# Parvovirus: Pathogenesis
what will now happen to normal host?
slight decreased Hgb level (slight anemia)
| not need any intervention
34
# Parvovirus: Pathogenesis
what will happen to host with chronic hemolytic anemia (immunocompromised hosts)
affect
their organs or result to chronic anemia
35
# Parvovirus
the entry post is also the?
exit point
36
# Parvovirus
how to acquire and infect other of the virus
Inhalation and exhalation
37
# Parvovirus
Once replication is done in the upper respiratory tracts what will happen?
manifestation will showL:
- Slapped cheeks
- Rash on the shoulder or other area of skin
- Joint pain (arthralgia)
38
# Parvovirus
does aplastic crisis in the bone marrow (femur) can happen if its replicated?
yes, it make kulang of RBC production or they hindi mature enough
39
# Parvovirus: Clinical Picture
From the start of infection, the aplastic crisis may coincide
with malais, what day?
8th day
40
# Parvovirus: Clinical Picture
signs and symptoms may not be present. what day
0-6th day
41
# Parvovirus: Clinical Picture
2nd-4th day what can happen
minute symptoms may be
experienced
42
# Parvovirus: Clinical Picture
when is the actual onset of symptoms, which will start the virion increase
6th day
43
# Parvovirus: Clinical Picture
Once the viremia starts to drop, IgM will increase , what day
10th day
| 10-12 day
44
# Parvovirus: Clinical Picture
Immediately after viremia, when the virions are at its lowest point, IgG will then
increase—usually starting on ?
16th day
## Footnote
However, once
IgG appears, rash and arthralgia may appear along with it.
45
Diagnosis for Parvovirus
- Serologic and DNA tests
- Diagnosed by presence of IgM (acute parvovirus)
- PCR or viral DNA from blood sample or in case of fetus from amniotic fluid
46
What are the other viruses that is included in the PaPoVavirus
- **Pa**pilloma (wart) viruses
- **Po**lyomavirus of mice
- **Va**cuolating (Simian virus 40, SV40)
47
# PaPoVavirus: Features
Kbp for polyoma and papilloma
- 5 kbp
- 8 kbp
48
PaPoVavirus envolped or not?
Nonenveloped or Naked
49
size and shape of PaPoVavrius
44 to 55 nm icosahedral symmetry
50
Transcriptional "____" first defined for papovaviruses
enhancers
51
what does papovavirus use to replicate the genome
| clue: an enzyme
host cell DNA polymerase
52
# PaPoVa: Papilloma virus
give the capsomeres and capsid proteins (major and minor_
- 72 capsomers
- 2 capsid proteins—1 major (L1 ) and
1 minor (L2)
53
# PaPoVa: Papilloma virus
Undergoes ____ for squamous epithelial cell resulting to a manifestation of warts
tropism
54
# PaPoVa: Papilloma virus
Common warts
Verrucae vulgaris
55
# PaPoVa: Papilloma virus
Plantar warts
Verrucae plantaris
56
# PaPoVa: Papilloma virus
Flat warts
Verrucae plana
57
# PaPoVa: Papilloma virus
Family:
Commona Name:
Virus:
- Papovaviridae
- Papilomavirus
- Human papiloma virus (HPV)
58
# PaPoVa: Papilloma virus
transmission
- Direct contact
- sexual contact for genital warts
59
# PaPoVa: Papilloma virus
Site of latency
Epithelial tissue
60
# PaPoVa: Papilloma virus
Oncogenic
Cervical and penile cancer (especially HPV types 16 and 18–for cervical canver onli)
61
# PaPoVa: Papilloma virus
Cervical cancer is cause by?
HPV - 16 and 18
62
# PaPoVa: Papilloma virus
cervical cancer is the?
**Squamous cell cancer** of the cervix
63
# PaPoVa: Papilloma virus
HPV-16 and HPV-18 can also manifest as?
vulvar cancer or penile cancer
| fuki and penis
64
# PaPoVa: Papilloma virus
warts present in the anal or genital area and is mainly caused by
HPV-6, and HPV-11
Condylomata acuminata (anogenital warts)
65
# PaPoVa: Papilloma virus
Condylomata acuminata (anogenital warts) is caused by what type of HPV
HPV-6, and HPV-11
66
# PaPoVa: Papilloma virus
Disorder:
HPV 1, 2, and 4
Plantar and common warts
67
# PaPoVa: Papilloma virus
Plantar and common warts can be removed through?
Electrocautery
68
# PaPoVa: Papilloma virus
HPV types:
skin cancer
HPV 5 and 8
69
# PaPoVa: Papilloma virus
HPV type:
common warts
HPV 7
70
# PaPoVa: Papilloma virus
HPV Types:
Genital warts and Respiratory papilloma
HPV 6 and 11
71
# PaPoVa: Papilloma virus
Disorder:
HPV 16, 18, 31, 33, 35, 45, and 56
Anogenital malignancies
| 16 and 18 most important
72
# PaPoVa: Papilloma virus
Diagnosis
Serology
PCR- DNA
Biopsy
73
# PaPoVa: Papilloma virus
Treatment
- Surgery
- Laser
- Cryosurgery
- Topical: Podophyllin and Idoxuridine (not common)
- electrocautery
74
# PaPoVa: Polyoma virus
Murine polyomavirus was isolated by and what year
Ludwig Gross in 1953
75
# PaPoVa: Polyoma virus
where did the murine polyomavirus was isolated?
Leukemia in mice
76
# PaPoVa: Polyoma virus
Named polyomavirus because it caused
solid tumors at multiple sites
77
# PaPoVa: Polyoma virus
2 virus
- BK virus [BKV]
- JC virus [JCV]
78
# PaPoVa: Polyoma virus
Site of latency
Kidney
79
# PaPoVa: Polyoma virus
Treatment
Supportive; decrease immune
suppression
80
# PaPoVa: Polyoma virus
Prevention
Avoid contact with virus; prevention of
acquisition of virus unlikely
81
# PaPoVa: Polyoma virus
isolated by Gardner et al in 1971 from urine of patient after
kidney transplant
BK VIRUS (BKV) or HUMAN POLYOMAVIRUS 1
82
# PaPoVa: Polyoma virus
who isolated BKV and what year
Gardner et al in 1971
| et al amputa
83
# PaPoVa: Polyoma virus
BK VIRUS (BKV) or HUMAN POLYOMAVIRUS 1 causes?
Renal Disease
84
# PaPoVa: Polyoma virus
BK VIRUS (BKV) or HUMAN POLYOMAVIRUS 1 are common in?
immunosuppressed kidney transplant patients
85
# PaPoVa: Polyoma virus
representaion of BKV to a children
Mild respiratory disease
86
# PaPoVa: Polyoma virus
BKV was isolated from?
Various tumors
87
# PaPoVa: Polyoma virus
Isolated by Padget et al in 1971 from brain tissue
JC VIRUS (JCV) or HUMAN POLYOMAVIRUS 2
88
# PaPoVa: Polyoma virus
who isolated, what year and where the JCV
- Padget et al
- in 1971
- brain tissue
89
# PaPoVa: Polyoma virus
since JCV is isolated from the brain tissue, what can you infer about how it affect the human
Affects the CNS, specifically the brain
| mag think ka nga
90
# PaPoVa: Polyoma virus
JCV or Human polyomavirus causes what
Progressive Multifocal Leukoencephalopathy
(PML)
91
# PaPoVa: Polyoma virus
JCV Progressive Multifocal Leukoencephalopathy (PML) involves a rare disease that affects the CNS
plaques of
demyelination/inflammation in the CNS
92
# PaPoVa: Polyoma virus
PML:
____ from these lesions are infected with JCV
Oligodendrocytes
93
# PaPoVa: Polyoma virus - pathogenisis
TOF
type of virus depends on their route of infection
T
94
# PaPoVa: Polyoma virus - pathogenisis
Generally, when the virus is inoculated in the respiratory
tract, what happens?
Multiply in the same area
95
# PaPoVa: Polyoma virus - pathogenisis
Multiplication in respiratory tract →
primary viremia
96
# PaPoVa: Polyoma virus - pathogenisis
after primary viremia, when there is a multiplication in kidney what will happen after
Transient secondary viremia
97
# PaPoVa: Polyoma virus - pathogenisis
Secondary viremia occure in immunocompetent
virus will remain **latent** indefinitely the kidneys
| basically - inactive
98
# PaPoVa: Polyoma virus - pathogenisis
when it is latent in the kidneys but the immune system fluctuates and made them **immunodeficient** what will happen
Reactivation of Virus
99
# PaPoVa: Polyoma virus - pathogenisis
what are the 2 paths for reactivation?
- BKV multiplies in Urinary tract
- JCV viremia
100
# PaPoVa: Polyoma virus - pathogenisis
BKV multiplies in Urinary tract →
viruria and possible hemorrhagic cystitis
| viruria - virus is present
## Footnote
presence of virus detected by RT-PCR
101
# PaPoVa: Polyoma virus - pathogenisis
JCV viremia →
Affects the CNS, will cause PML
102
# PaPoVa:
VACUOLATING VIRUS other name
SV-40
103
# PaPoVa: Vacuolating
hawk
## Footnote
r
tuuAAHHH
104
# PaPoVa: Vacuolating
TOF
Ubiquitous in humans
T
| Ubiquitous = present
105
# PaPoVa: Vacuolating
TOF
Ubiquitous in humans, but have NOT BEEN ASSOCIATED
WITH DISEASE
T
106
# PaPoVa: Vacuolating
Reside in the?
BrAIIINN
| zombies phat ass
107
# PaPoVa: Vacuolating
have been associated with?
**rare encephalopathy** in immunocompromised individuals
108
# PaPoVa: Vacuolating
isolated by and in what year
Sweet and Hilleman in 1960
109
# PaPoVa: Vacuolating
Isolated by Sweet and Hilleman in 1960 in
primary monkey
kidney cells cultures
110
# PaPoVa: Vacuolating
primary monkey
kidney cells cultures being used to grow?
Sabin OPV Vaccine
111
# Adenovirus
Name originates from Greek word, which means?
“adenas” which means
gland
112
# Adenovirus
site from which were initially isolated
gland
113
# Adenovirus
facilitate binding onto host
Fibers protruding from capsid
114
# Adenovirus
All human serotypes are included in a single genus within the
family ____
Adenoviridae
115
# Adenovirus
Latent infection in
- tonsils
- adenoids
116
# Adenovirus
Outbreaks mostly occur in
- military recruits
117
# Adenovirus
Transmissio
Respiratory, fecal-oral, and direct
contact (eye
118
# Adenovirus
Site of Latency
Replication in oropharynx
119
# Adenovirus
Prevention
Vaccine (adenovirus serotypes 4 and
7)
120
# Adenovirus
All human Adenoviruses share this common group-specific antigen
Cross reactive protein- HEXON
121
# Adenovirus
TOF
print specific antigens are important in serotyping
F (TYPE not PRINT)
122
# Adenovirus
isolated in what year and from?
- 1935
- Human adenoid tissue
123
# Adenovirus
how many antigenic types were isolated from human and animals?
49 distinct antigenic types
124
# Adenovirus
Spread by?
o Direct contact
o Respiratory droplets
o Fecal-oral
125
# Adenovirus
Infect and replicate on?
Epithelial cells of:
- Pharynx
- Conjunctiva
- Urinary bladder
- Small intestine
126
# Adenovirus
The virus has a tendency to become latent in?
lymphoid tissue
127
# Adenovirus
when latent, how it can be reactivated?
by immunosuppression
128
# Adenovirus
portal of entry or first infection
eye and upper resipartory
129
# Adenovirus
eye and upper resipartory →
upper respiratory
130
# Adenovirus
Upper respiratory →
Lower respiratoy or Gastrointestinal
131
# Adenovirus
Lower respiratoy or Gastrointestinal →
lymph nodes
132
# Adenovirus
when in lymph nodes, whAT happens to the virus?
becomes latent, but if immucompromised will activate
133
# Adenovirus
Lymph node →
undergo viremia
134
# Adenovirus
Viremia →
- Skin
- Multiple organs
| Manifestation
135
# Adenovirus
Skin and multiple organs →
Resolution or latency
136
# Adenovirus
no.1 cause of?
Viral conjunctivitis
137
# Adenovirus
Serotype:
Acute Respiratory Disease (ARD)
Serotype 3,4,7,21
138
# Adenovirus
Disease:
Stereotype 8,19
epidemic Keratoconjunctivitis
139
# Adenovirus
Serotype:
Hemorrhagic Cystitis
Serotype 11 and 21
140
# Adenovirus
Disease:
Serotype 40 and 41
Infantile Gastroenteritis
141
# ADENOVIRUS
STUDY THE PATIENT POPULATION
di naman daw to need kabisaduhin, pero ikaw bahala buhay mo naman yan kaya mo nayan ang laki mong bulas sa mundo
142
# Adenovirus
Virus particle by Electron microscopy can be detected by direct examination of
Fecal extract
143
# Adenovirus
Detection of adenoviral
- antigens by ELISA
- Nuclear antigen by PCR
144
# Adenovirus
Diagnosis:
cell culture detects
grape like cytopathic effect
145
# Adenovirus - diagnosis
media used for cell culture
Media: HEK, PMKC, Hep-2, KB, HeLa
146
# Adenovirus - diagnosis
isolation depending on the clinical disease, the virus may be
- Throat
- Conjunctival swabs
- Urine
147
# Adenovirus - diagnosis
solation is much more difficult from
- Stool
- rectal swab (sarap)
148
adenovirus
shakes their ass, u should too
