Bio150: Exam #2 Review Sheet Flashcards

1
Q

What is the Surface Area-To-Volume Ratio?

A

As the cells size increase, the surface increases by a squared power and the volume increases by a cubed power.

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2
Q

What do Compound Microscopes do?

A
  • Magnify in stages using several lenses

- Resolve structures by at least 200 nanometers

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3
Q

What do Transmission Microscopes do?

A
  • Transmits electrons through material

- Resolves objects only 0.2 nanometers apart

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4
Q

What do Scanning Electron Microscopes?

A
  • Beams electrons onto surface of specimen and reflect back
  • Specimen amplifies and transmits the reflected electrons
  • Yields 3D images
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5
Q

Facts about Prokaryotes.

A
  • Encased in a Cell Wall
  • All cellular functions occur within the same area in the cell (one room)
  • Bacterial Cell Walls contain Peptidoglycan
  • Archaean Cells Walls lack Peptidoglycan and have polysaccharides and proteins
  • Move by flagella
  • Instead of Nucleus there is a Nucleoid
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6
Q

Facts about Eukaryotes.

A
  • Compartmentalized by the endomembrane system
  • Supported by Cytoskeleton
  • Has Nucleus
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7
Q

What occurs in the Nucleolus?

A

Intensive synthesis of ribosomal RNA.

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8
Q

What is the Nuclear Envelope?

A

The two phospholipid bilayer membranes that bind to the surface of the nucleus.

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9
Q

What are Nuclear Pores and what do they do?

A
  • Structures that are scattered over the surface of the Nuclear Envelope
  • Allow ions and small molecules to diffuse freely between nucleoplasm and cytoplasm
  • Controls passage of proteins and RNA-protein complexes
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10
Q

Facts about Chromatins.

A
  • They are composed by chromosomes that are organized with proteins
  • Structure affects the function of DNA
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11
Q

Facts about Ribosomes.

A
  • Composed of ribosomal RNA and Proteins
  • Only function when actively synthesizing proteins
  • Process requires messenger and transfer RNA
  • They are the factories that make proteins
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12
Q

Facts about Endoplasmic Reticulum.

A
  • Composed of a phospholipid bilayer embedded with proteins

- Two largest components are the Cisternal Space (Lumen) and Cytosol (fluid component of cytoplasm)

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13
Q

Rough Endoplasmic Reticulum Facts.

A
  • Composed primarily of flattened sacs
  • Surface is studded with ribosomes
  • Proteins from here are exported to lysosomes, vacuoles, or plasma membrane
  • Glycoproteins modify new proteins
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14
Q

Smooth Endoplasmic Reticulum Facts.

A
  • Composed primarily of tubules
  • Membranes are embedded with enzymes
  • Enzymes create carbohydrates and lipids
  • Steroid Hormones are made in SER as well
  • Storage of Ca2+
  • Ca2+ triggers muscle contraction and is used for signaling pathways
  • Performs detoxification
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15
Q

Golgi Apparatus Facts.

A
  • Composed of flattened stacks of membranes
  • Stacks are called Cisternae
  • Functions in collection, packaging and distribution of molecules
  • Cis side of GA is the receiving end
  • Trans side of GA is the shipping end
  • Synthesizes cell wall components (non-cellulose polysaccharides)
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16
Q

Lysosome Facts.

A
  • Digestive vesicles that arise from Golgi Apparatus
  • Contain degrading enzymes that catalyze the rapid breakdown of organic molecules (proteins, lipid, carbohydrates and nucleic acids)
  • Break down old organelles and recycles their components
  • Function in acid pH and activated by phagocytosis of food vesicles
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17
Q

Peroxisomes Facts.

A
  • Contains enzymes that oxidize fatty acids

- The enzyme “Catalase” breaks down hydrogen peroxide to make it harmless

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18
Q

What is the Tonoplast?

A

The membrane surrounding the Central Vacuole that determines the cells tonicity (osmotic balance).

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19
Q

Mitochondria Facts.

A
  • Typically tubular or sausage-shaped found in Eukaryotic Cells
  • Bound by smooth outer membrane and inner folded membrane with Cristae
  • Cristae contains matrix and intermembrane space
  • Have their own DNA
  • Participates in Oxidative Metabolism
  • Mitochondria divides on its own accord
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20
Q

Chloroplast Facts.

A
  • Make their own food

- Contain chlorophyll (green pigment)

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21
Q

Centrosome Facts.

A
  • Surrounds Centrioles
  • Reorganizes microtubules during cell division
  • Centrosomes of plants and fungi lack centrioles
22
Q

What are the 3 types of fibers that make up the Cytoskeleton?

A
  • Actin (Microfilaments)
  • Microtubules
  • Intermediate Filaments
23
Q

What are Actin Filaments?

A
  • Long fibers (7nm)
  • Composed of two protein chains which are made up of actin
  • Exhibit polarity (have + and - ends)
24
Q

What are Microtubules?

A
  • Largest of cytoskeletal elements; Hollow tubes (25nm)
  • Composed of 13 protein protofilaments
  • Protofilaments are made of alpha and beta tubulin subunits
  • Facilitate cellular movement
  • Organizes the cytoplasm
  • Responsible for moving materials within the cell itself
25
Q

What are Intermediate Filaments?

A
  • Most durable element of cytoskeleton (8-10nm)
  • Stable and do not break down
  • Keratin (Intermediate Filament) is found in epithelial cells (hair, nails, etc)
26
Q

Flagella (Cilia) Facts.

A
  • Cellular extensions with 9+2 arrangement of pairs of microtubules
  • Motility or moving fluids over surfaces
27
Q

Extracellular Matrix Facts.

A
  • Mixture of glycoproteins surrounding animal cells
  • Attached by fibronectin
  • Integrins allow ECM to influence cell behavior
  • Integrins can alter gene expression and cell migration patterns
28
Q

Facts about Desmosomes.

A
  • Cadherin-based Junction (in Vertebrates)

- Creates strong, flexible connections between cells

29
Q

Tight Junction Facts.

A
  • Unique to vertebrates
  • Contain Claudins (proteins)
  • Occlude or Block substances from passing between cells
30
Q

Gap Junction Facts.

A
  • Found in both invertebrates (Pannexins) and vertebrates (Pannexins and Connexons)
  • Provide passageways large enough to permit small substances from one cell to the next
31
Q

Plasmodesmate Facts (in plants).

A
  • Cytoplasmic Connections between adjacent cells
  • Lined with plasma membrane
  • Contain central tubule that connects the endoplasmic reticulum of two cells
32
Q

What is the Fluid Mosaic Model and what does it do?

A
  • Mosaic of proteins

- Floats in or on the fluid lipid bilayer like boats on a pond

33
Q

What is the purpose of the Phospholipid Bilayer?

A
  • Imposes a barrier to permeability

- Provides flexible matrix

34
Q

What do Transmembrane Proteins do?

A

Provide transport and communication across the membrane.

35
Q

What happens in Facilitated Diffusion?

A

Aquaporins allow water to diffuse in and out of cells through proteins.

36
Q

What happens in Endocytosis?

A

Cells extend membranes and engulf what it eats and turns it into a vesicle.

37
Q

What happens in Exocytosis?

A

Vesicles attaching to the plasma membrane release larger molecules out of cell (requires energy).

38
Q

Glycocalyx Facts.

A
  • Determines blood type
  • Sugar-coating over cells
  • Similar to cell wall
39
Q

What are the 3 types of Endocytosis?

A
  • Phagocytosis (Matter)
  • Pinocytosis (Fluid)
  • Receptor-mediated
40
Q

What does a catalyst do?

A

Lowers activation energy.

41
Q

What are Competitive Inhibitors?

A

Substances that compete with substrate for active sites on Enzymes.

42
Q

What is the First Law of Thermodynamics?

A
  • Energy cannot be created or destroyed

- Energy remains constant in the universe

43
Q

What is the Second Law of Thermodynamics?

A

States the entropy is increasing.

44
Q

What are Non-Competitive Inhibitors?

A

Bind to enzyme in the allosteric site, changing the shape of enzyme and making it unable to bind to substrate.

45
Q

What does an Allosteric Activator do?

A

Binds to allosteric sites to keep an enzyme active.

46
Q

Fermentation Facts.

A
  • Recycles NAD+

- Use of organic molecules as final electron acceptor

47
Q

What are the steps in Glycolysis?

A
  • Priming Reactions (Investment)
  • Cleavage (Splitting into two)
  • Payoff
48
Q

Where does Glycolysis happen?

A

In cytoplasm.

49
Q

Where does the Krebs’ Cycle occur?

A

In mitochondria.

50
Q

Where does Pyruvate Oxidation occur?

A

In mitochondria.

51
Q

How much ATP does each NADH and FADH2 produce?

A
  • 3 ATP

- 2 ATP