Antigen-Antibody/Immunological Reaction Test

Question 1

Definition of serological test

412

Answer 1

The tests used to detect Atg/AB in serum

412

Question 2

Dangerous to culture ??

Answer 2

Rickettsial D

412

Question 2

Use of serological test ??

Answer 2

1. Dx of infectious D
2. Dx of autoimmune D
3. Determination of Blood type
4. Determination of HLA type

412

Question 2

Why Atg detection is superior to AB detection ?

Answer 2

Atg is more specific for Dx of D if Atg is detected it indicates the presence of target pathogen

412

Question 3

Which organisms cannot culture ??

Answer 3

1. Syphilis
2. Hepatitis-A B C

412

Question 4

Take to much time to grow ??

Answer 4

Mycoplasma

412

Question 5

Dx of autoimmune D ??

Answer 5

1. SLE=Ab against DNA
2. Rheumatoid arthritis = Ab against IgG

Question 5

Culture tecnique not readily available?

Answer 5

HIV
EBV

Question 6

Name of Ag-Ab reaction ??

Answer 6

1. Agglutination test
2. Precipitation test
3. Complement fixation test= CFT
4. Immunoassay using labeled reagents

413

Question 6

Name of agglutination test ??

ST LPH

Answer 6

1. slide
2. tube
3. latex
4. particle
5. Hemagglutination

413

Question 7

Complement fixation test use ?

Answer 7

Kala azar
Filarial parasite
Gonococcus

Question 8

Immunoflurescence IF use ?

Answer 8

Detection of T B Cells
Detection of treponemal AB in syphilitic patients

Question 9

Enzyme Linked Immuno Sorbent Assay ELISA use ?

Answer 9

HIV ab
Hepatitis B panel
HBsAg HBeAg anti hbs anti hbc

Question 10

Direct agglutination test ??

Answer 10

1. Slide
2. Tube

414

Question 11

Slide agglutination tets ??

Answer 11

1. Blood grouping
2. Serotype of bacteria

414

Question 11

Tube agglutination test ??

Answer 11

1. Widal test-Enteric fever
2. Weil-Felix test-Rickettsial infection

414

Question 12

Latex ??

Answer 12

1. HBsAg test
2. ASO titre estimation

414

Question 12

Indirect/Passive agglutination test ??

Answer 12

1. Lat3ex
2. Paticle
3. Hemagglutination
4. Co-agglutation

414

Question 13

Particle ??

Answer 13

Anti HIV ab

Question 14

Hemagglutination??

Answer 14

TPHS for Dx of syphillis

414

Question 15

Co-agglutination ??

Answer 15

atg from CSF in case of bacterial meningitis

414

Question 16

Indirect Hemagglutination test ??Procedure ??

Answer 16

RBC + Tannic acid/CrCl2 — (Tagged Atg)—-Pt serum —Serum contain AB – IF AB there will be hemagglutination
reverse can be occure
RBC= Sensitized RBC

415

Question 16

Use of coombs test ??

Answer 16

1. Hemolytic disease
2. Autoimmune hemolytic anemai

415

Question 17

Indirect HAG test ??

Answer 17

1. Treponema pallidum H-TPHA -Syphillis
2. Reverse passive H -RPHA - Hepatitis B

415

Question 18

Prozone phenomenon definition??

Answer 18

Inhibition of agglutination by excess AB | 415

Question 19

M/A of PP ??

Answer 19

Each epitope of Atg is occupied by one AB & there is no clumping of Ag - so no agglutination | 415

Question 21

How to overcome PP ??

Answer 21

Dilutions of AB are added to identical conc of particulate Atg | 415

Question 22

Agglutination definitoion ??

Answer 22

Atg= IS particulate or is an inert particle coated with an atg AB=cross react with Atg=Latticework formed = Clumping | 416

Question 23

Precipitation def ??

Answer 23

Atg in solution AB cross react with Atg & aggregates (precipitates) form | 416

Question 23

For the quantitation of either atg / ab in pt specimen which test ??

Answer 23

ELISA | 418

Question 24

Which ELISA detect Atg ??

Answer 24

1. Sandwich 2. Competitive | 419

Question 24

ELISA procedure ??

Answer 24

1. Bottom of the well=ATG 2. Atg --- Pt serum AB 3. Pt serum AB --- AB to human IgG 4. AB to Human IgG --- Enzyme 5. Enzyme --- Substarte 6. Sub change the colour when it is acted upon by the E | 419

Question 25

Types of ELISA ??

Answer 25

1. Sandwich 2. Competitive 3. Direct 4. In direct | 419

Question 26

precipitation in solution example ??

Answer 26

1. Detection of AB 2. Detection of Atg | 416

Question 26

Use of ELISA ??

Answer 26

1. Hepatitis B 2. HIV AB 3. Toxoplasma AB | 419

Question 27

Immunoflurescene procedure ?? | Fluroscence AB test

Answer 27

1. Known Atg is bind to the slide 2. Pt serum 3. If pt serum contain AB -it will stict to the atg -- 4. seen fluorescent dye-labeled AB to human IgG | 419

Question 28

Precipitation in agar example ??

Answer 28

1. measure IgG IgM 2. measuree C3 3. measur Creactive protein-CRP | 416

Question 29

Precipitation in agar with an electric field??

Answer 29

1. H influenza 2. N meningitis 3. S pneumoniae | 416 Dx

Question 30

CFT in (+) reaction ??

Answer 30

Atg + AB (if present ) = complement fix 2nd stage sensitized RBC ---- no lysis --no hemolysis

Question 30

CFT (-) test ??

Answer 30

Atg + AB (NO) = complement fix NOT 2nd stage sensitized RBC ---- lysis -- hemolysis