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Vibrio Cholerae Flashcards

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1
Q

Biotypes of Vibrio cholerae O-1 ??

Difference in biological/biochemical reactions

A
  1. Classical
  2. E1-Tor

242

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2
Q

Serotypes of Vibrio cholerae (Classical )??

Antigen difference

A
  1. Inaba
  2. Ogawa
  3. Hikojima

242 IOH

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3
Q

Serotypes of Vibrio Cholerae (E1-Tor) ?

A
  1. Inaba
  2. Ogawa
  3. Hikojimu

242

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4
Q

Appearance of Vibrio cholerae on Gram Stain ??

A

Curved Comma shaped

242

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5
Q

Biochemical characteristics of V C ??

A

Oxidase (+) which differentiate them from enteric gram (-) bacteria

243

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6
Q

Which colour is V C

A

Blue
Oxidase (+)

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6
Q

Virulence factor of VC ??

A
  1. Enterotoxin
  2. Pili
  3. Mucinase

243

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7
Q

Toxin name of V C ??

A

Enterotoxin - Choleragen

243

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7
Q

VC vs Enterobacteriaceae ??

A
  1. Single polar flagella
  2. Curved bacillus
  3. Oxidase (+)
  4. Aerobe

Opposite of enterobacteriaceae

243

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8
Q

Hnaging drop preparation appearance V C ??

A

Fish in stream in appearance

243

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9
Q

Antigen structure of V C ??

A
  1. O atg
  2. H atg

243

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10
Q

Media for V C ??

A
  1. Thiosulphate citrate bile sucrose TCBS agar
  2. Monsurs media - Tellurite Taurocolate Gelatin AgAR MEDIA
  3. MacConkey agar
  4. Nutrient agar

243

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11
Q

TCBS media appearance of VC ??

A
  1. Yellow colony
  2. Background- Dark-Green

243

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11
Q

Monsurs media appearance of VC ?

A
  1. Dark colony
  2. Characteristics hollows

243

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12
Q

BD stain of V C ??

A

V C O139 Bengal

245

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12
Q

High risk of V C infection ??

A
  1. Person have little/No Gastric acid - Take antacid
  2. Who had - Gastrectomy
  3. Blood group- O

244

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13
Q

Subunit of Choleragen ??

A
  1. A = Active subunit
  2. B = Binding subunit

244

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14
Q

A subunit function ??

A

ADP- Riborylation
Enters into the cell

244

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15
Q

B subunit function of Cholerageen ??

A

Binds to a ganglioside receptor of enterocyte
GM1 = Activate Gs Pathway

244

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16
Q

Why watery D in cholera ??

A
  1. C is not an invasive infection
  2. Choleragen toxin
  3. Inc secretion of Cl & H2O from the cells
  4. Watery D = No RBC or Neutrophills

244

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17
Q

A subunit further action ??

A
  1. Stimulate adenyl cyclase
  2. Overproduction of cAMP
  3. pKa = Protein kinase = Activate transport channel = Cystric fibrosis

244

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18
Q

Hallmark of cholera infection ??

Study These Flashcards
A

Watery diarrhea

245

19
Q

MacConkey agar characteristics in V C ??

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A

Colorless
Lactose fermented slowly

245

20
Q

Dark ground illumination DGI appearance of V C ??

Study These Flashcards
A

Shooting star in dark sky

245

21
Microscopic examination of V C ??
1. Gram staining 2. Hanging drop preparation 3. DGI | 245
22
TSI agar characteristics??
1. Acid slant 2. Acid butt without gas / H2S 3. Ferments sucrose | 245
23
Why monsur media dark black colony??
Liquifaction of gelation | 245
24
Biochemical characteristics of V C ??
1. Urease (-) 2. Oxidase (+) 3. Reduce nitrate 4. Produce indole 5. Fermant sucrose & lactose 6. No gas | Concept
24
Tx of cholera ??
Azithromycin | 246
25
Vaccine of cholera ??
Vaxchora Live attenuated vaccine + Tetracycline =Close contact
26
Transport media for VC ??
1. Alkaline peptone water 2. Carry Blair Media | Concept
27
Clinical features of V C ??
1. Rice watery stool - 2. Dehydration 3. Cardiac F 4. Renal F 5. Acidosis 6. Hypokalemia 7. No Abdominal pain 8. 40% case death | 246
28
Toxins act by inc intracellular cAMP ??
1. Cholera toxin 2. Labile T of E coli 3. Pertusis T 4. Edema factor of anthrax T | Concept
28
Never casue extra intestinal infection ??
1. V C 2. Shigella | C
29
Serological test for VC ??
1. Slide agglutination test 2. Rise in AB titre
30
why TTGA media selective ??
bile salt K tellurite
31
why indicatior TTGA media ?
K tellurite
32
Findings of TTGAM ??
central black colony sourrounded by clear halo
33
why clear halo ?
liquefaction of gelatin
34
why central black colony <<
metallic tellurium
35
name of storage media ??
egg saline media chalk cooked meat broth | 7-2-2025 12.32 PM
36
which organization in BD work in cholera <
icddrb
37
ICDDRB full form ??
International Centre for Diarrhoeal Disease Research, Bangladesh
38
how to prepare monsurs media for vibrio cholera
Dr. Monsur, in the preparation of **Monsur's medium**, used specific ingredients to create a selective and differential medium for isolating *Vibrio cholerae*. The key components he used include: 1. **Peptone** and **Gelatin**: As nutrient sources for bacterial growth. 2. **Sodium Chloride (NaCl)**: To provide an optimal saline environment for *Vibrio* species, which are halophilic. 3. **Potassium Tellurite**: As a selective agent to inhibit the growth of most bacteria except *Vibrio* species. 4. **Sodium Taurocholate**: To enhance the growth of *Vibrio cholerae* and inhibit non-cholera vibrios. 5. **Agar**: As a solidifying agent. The medium was adjusted to an alkaline pH (8.5–9.0) to favor the growth of *Vibrio cholerae*, which thrives in alkaline conditions. Potassium tellurite also acts as a differential agent, causing *Vibrio cholerae* colonies to appear gray or black due to tellurite reduction.
39
5 common example of oxidase positive bacteria ?
Oxidase-positive bacteria are those that produce the enzyme **cytochrome c oxidase**, which is detected using oxidase tests. Here are 5 common examples: 1. **Pseudomonas aeruginosa** 2. **Neisseria gonorrhoeae** 3. **Vibrio cholerae** 4. **Campylobacter jejuni** 5. **Aeromonas hydrophila** These bacteria are often identified in clinical and environmental microbiology using oxidase tests as a key diagnostic tool.
40
Why shooting star in dark sky ?
In Dark ground illumination - rapidly motile rods
41
enterotoxin name of Vibrio ?
Choleragen
42
Oxidase kemon vibrio er ?
+
43
name some oxidase + bacteria ?
**🦠 Oxidase-Positive Bacteria 🔬** ✔ **Gram (-) Rods** ➡ *Pseudomonas aeruginosa* ➡ *Vibrio* spp. (e.g., *V. cholerae*) ➡ *Campylobacter jejuni* ➡ *Helicobacter pylori* ✔ **Gram (-) Diplococci** ➡ *Neisseria* spp. (*N. meningitidis, N. gonorrhoeae*) ✔ **Curved Gram (-) Rods** ➡ *Aeromonas* spp. ➡ *Pasteurella multocida* 🚨 **Dx:** **Oxidase test (+) → Purple color reaction** @usmlereviews
44
name of some catalase positive bacteria ?
**🦠 Catalase-Positive Bacteria 🔬** ✔ **Gram (+) Cocci** ➡ *Staphylococcus* spp. (**S. aureus, S. epidermidis, S. saprophyticus**) ✔ **Gram (-) Rods** ➡ *Pseudomonas aeruginosa* ➡ *E. coli* ➡ *Klebsiella* spp. ➡ *Salmonella* spp. ➡ *Serratia* spp. ✔ **Other Catalase (+) Bacteria** ➡ *Listeria monocytogenes* ➡ *Campylobacter jejuni* ➡ *Helicobacter pylori* ➡ *Nocardia* spp. 🚨 **Dx:** **Catalase test (+) → Bubbles (H₂O₂ breakdown)** @usmlereviews
45
Kivabe fish in stream dekhba ?
Hanging drop preparation
46
why Vibrio not a enterobacteriaceae ?
oxidase + motile - monotrichus flagella grow in alkaline pH curved bacilli
47
shape of vibrio ?
comma shaped

Microbiology -Medical-Prof (49 decks)

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