Enterobacteriaceae

Question 1

common characteristics of enterobacteriaceae ??

Answer 1

• facultative anaerobes
• oxidase -
• fermant glucose
• reduce nitrates to nitrites

219

Question 2

other features of E ?/

Answer 2

• G - rods
• motile with peritrichous flagella
• catalase +
• grow-bile containing MacConkey agar media

219

Question 3

only capsulated E ??

Answer 3

klebsiella

219

Question 4

Non motile E ??

Answer 4

SKY
Shigella
Klebsiella
Yersinia

219

Question 4

lactose fermented rapidly E ??

Answer 4

Klebsiella
E coli
Enterobacter

Question 5

lactose non-fermenter ?/

Answer 5

shigella
salmonella
proteus

Question 6

Lactose F slowly ?/

Answer 6

serratia
vibrio

Question 7

why Pseudomonas is not E >??

Answer 7

aerobe
doesnot ferment glucose
oxidase +
donot reduce nitrates

220

Question 8

Lactose + colour in Macconkey ??

Answer 8

pink

Question 9

colourless colony ?/

Answer 9

shigella
salmonelaa
proteus

Question 10

Pink colony by which E in macConkey media ??

Answer 10

e coli
\klebsiella
citobacter
E

Question 11

Diseases caused by Escherichia Coli ??

Answer 11

223

Question 12

source of E coli in UTI ?/

Answer 12

Colonic flors

Question 13

source of e coli in neonatal meningitis ??

Answer 13

mothers birth canal

Question 14

properties of E coli ?

Answer 14

222

Question 15

E coli VS Shigella /Salmonella ?/

Answer 15

SS = Donot ferment lactose

Question 16

UTI by E coli >???

Answer 16

223

Question 17

endotoxin mediated diarrhea causing baccteria ??

Answer 17

E coli
Vibrio cholerae

Question 18

Invasive inflammatory Diarrhea causing bacteria ??

Answer 18

shigella
salmonelaa\
compylobacteer jejuni
E coli O157:H7

Question 19

Classification of E coli ??

Answer 19

Enteropathogenic
Enterotoxigenic
Enteroinvasive
Enterohemorrhagic
Enteroaggregative

224

Question 20

why UTI common in women ?/

Answer 20

short urethra
proximity of urethra to the anus
colonization of the vagina by member of fecal flora

Question 21

enterohemorrhagic E coli diseases name ?

Answer 21

• Bloody Diarrhea
• Hemorrhagic colitis
• Hemolytic uremic syndrome

226

Question 22

why name shiga toxin ??

Answer 22

same toxin as shigella

Question 23

e coli Butt & slant colour ?

Answer 23

Yellow
Yellow

Question 24

M/A of diarrhea by E coli ?/

Answer 24

224

Question 25

MacConkey agar media sterilization ??

Answer 25

Tyndalization - 100

Question 26

anti-microbial susceptibility test name ?/

Answer 26

mueller hinton agar media disk diffusion dilution methos

Question 27

Muller hinton media type <<

Answer 27

solid basic media

Question 28

M H media sensitive to what ??

Answer 28

Clear zone of inhibition

Question 29

Antibiotic disc in M H media ?/

Answer 29

Inhibit growth of bacteria

Question 30

enteropathogenic E coli cause ?

Answer 30

bloody D

Question 31

Travelers D cause by ??

Answer 31

Entertoxigenic E coli

Question 32

Tx of O157:H7 ??

Answer 32

ciprofloxacin

Question 33

E coli VS lactose fermenting other G - rods ?/

Answer 33

* produce indole from tryptophan decarboxylate lysine source of carbon - acetate motile

Question 34

Mode of transmission of EHEC ?

Answer 34

undercooked hambuerger fast food restaurant unpasturized juices contact with animals - O157:H7

Question 35

M/A of shiga toxin ?

Answer 35

226

Question 36

how to prevent hemorrhagic collitis ?

Answer 36

cooking ground beef 28.2.2025 6.28PM

Question 37

UTI kara kore ?

Answer 37

E coli enterobacter proteus serratia klebsiella

Question 38

Enterobacteriaceae ki ki ache ??

Answer 38

E coli shigella salmonella klebshiella proteus serratia

Question 39

Bacteroides aerobic or anaerobic ?

Answer 39

anaerobic 291

Question 40

why Exotoxins of E coli are called Heat labile and heat stable ? meaning of this ?

Answer 40

🔬 **Heat-Labile (LT) vs. Heat-Stable (ST) Exotoxins in *E. coli*** - **💨 Heat-Labile (LT):** Denatured @ **>60°C** - Activates **Gsα → ↑cAMP** - ⬆ Cl⁻ secretion, H₂O efflux → **Watery diarrhea** - Similar to **Cholera toxin** - Encoded by **plasmid** - **🔥 Heat-Stable (ST):** Resistant to heat **(Stable @ 100°C)** - Activates **Guanylate cyclase → ↑cGMP** - ⬇ Na⁺ reabsorption → **Watery diarrhea** - Types: **STa (human) & STb (animal)** - Encoded by **plasmid or transposon** 🦠 **Diseases:** Traveler’s diarrhea (ETEC) 💊 **Tx:** Supportive, rehydration, ± fluoroquinolones @usmlereviews

Question 41

MC media type ?

Answer 41

selective indicator

Question 42

why MC indicator ?

Answer 42

neutral red

Question 43

E coli Virulence factors

Answer 43

LT ST Endotoxin verotoxin capsule pili

Question 44

Ekjon Pt aslo - UTI suspect korcho ?? ekhon kivabe approach korba ?

Answer 44

Urine culture - whole 769

Question 45

which bacteria is suspected if colour of macconkey agar media in translucent ?

Answer 45

🦠 **MacConkey Agar Interpretation** 🔍 **Translucent/Colorless Colonies** = **Non-lactose fermenter (NLF) → Possible Pathogen** 🚨 **Suspected Bacteria** (Gram-negative rods): 1️⃣ **Salmonella** 🥚 (H2S + on TSI) 2️⃣ **Shigella** 💩 (H2S -) 3️⃣ **Proteus** (Swarming, Urease +) 4️⃣ **Pseudomonas aeruginosa** 💚 (Oxidase +, Fruity odor) 🧪 **Confirmatory Tests:** TSI, H2S, Urease, Oxidase, API @usmlereviews

Question 46

how urine sample collected ?

Answer 46

early morning mid-stream urine sample - 5ml - clean dry sterile screw cap container - sterelized test tube urine sample should be properly labeled to avoid contamination - urine should be collected after proper cleansing of genitalia