Bacteriology - Pyogranulomas Flashcards

(56 cards)

1
Q

What type of cells will be seen in acute inflammation?

A

neutrophils

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2
Q

Pyogenic bacteria causes suppurative inflammation primarily acting as __________

A

extracellular bacteria

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3
Q

Underlying disease process is usually of one ________ and ________

A

suppuration and abscess formation

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4
Q

What are the 4 main types of pyogenic bacteria?

A

staphylococci
streptococci
cornyebacteria
trueperella

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5
Q

Which bacteria are a mixture of acute and chronic inflammation?

A

pyogranulomatous

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6
Q

How do pyogranulomatus cause inflammation?

A

by acting both extra and intracellularly

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7
Q

How can pyogranulomatus avoid being killed?

A

evading intracellular destruction

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8
Q

How can we overcome bacteria evading intracellular destruction?

A

through cell mediated immunity which can be achieved by vaccines

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9
Q

What are the rules of thumb regarding Gram positive rods

A

less frequent isolated than other groups of bacteria
tend to cause syndromes - can give presumptive diagnosis
if more information is needed definitive diagnosis is needed

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10
Q

What are the 3 main types of actinomycetes?

A

actinomycetes
nocardia
dermatophilus

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11
Q

What is an important diptheroid gram positive bacteria?

A

rhodococcus

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12
Q

What are the oxygen requirements of
Actinomycetes
Nocardia
Dermatophilus

A

A - FA or ANO2
N - strict aerobes
D - strict aerobes

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13
Q

What are the 3 main characteristics of pyogranulomatous

A

gram +
filamentous
branching rods

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14
Q

What are distinguishing features of rhodococcus?

A

gram +, short club shaped rods

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15
Q

What is meant by actinomyces being an opportunistic disease?

A

requires something to allow access of organism into diseased sit and disease is NEAR where they are normal flora

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16
Q

Most species of actinomyces are normal flora of what two areas?

A

oral cavity and GIT

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17
Q

Which Actinomycetes is considered to be a saprophyte? What does this mean?

A

Saprophytes
found in soil and decaying vegetation

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18
Q

While nocardia is considered to be opportunistic, it is considered to be a ______ pathogen. What does this mean?

A

poor
requires significant compromise

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19
Q

Which Actinomycete is considered to be an obligate parasite of the skin but can survive in the environment for a long time

A

dermatophilus congolensis?

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20
Q

Who is considered to be a reservoir to dermatophilus congolensis?

A

carrier animals or fomites

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21
Q

Which Actinomycete requires alteration of the skin for invasion, typically wetting?

A

Dermatophilus congolensis

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22
Q

How can dermatophilus be transferred from an infected/carrier animal to a susceptible one?

A

rub against each other (contagious)
transferred by insects (flies, ticks)
by contaminated fomites (scabs, sheep dips, brushes)

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23
Q

Where do Rhodococci equi come from?

A

saprophytes
found in soil
secondary in GIT of horses
animal manure
inhalation or ingestion

24
Q

What are the virulence factors of nocardia?

A

mycolic acid and cell wall lipids

25
What are the virulence factors of dermatophilus?
motile zoospores aid invasion
26
What are the virulence factors of Rhodococcus?
plasmid mediated, VapA
27
Which two of the Actinomycetes are facultative intracellular parasites?
nocardia Rhodococcus
28
What "syndrome" does actinomyces bovis cause? In what species?
Lumpy Jaw in cattle
29
What does lumpy jaw require to cause disease?
some alteration to mucusa/skin to allow access of organism into site of disease production
30
What are some examples of mucosal/skin alteration that allows actinomyces bovis to gain access into organism?
grass awns sharp feed impacted feed
31
What does Lumpy Jaw result in?
chronic pyogranulomatous osteomyelitis of the mandible or maxilla
32
What clinical signs are seen with lumpy jaw? What are the signs caused by?
hard, non-painful lumps on the head of cattle caused by impairment of function
33
What 3 "syndromes" will actinomyces spp cause? (Including Actinomyces bovis)
lumpy jaw abscesses serositis
34
What are abscesses?
pyogranulomatous lesions
35
What do abscesses occur secondary to?
bite or traumatic injury
36
What flora are involved in abscesses?
mixed
37
What is serositis? What is it caused by? What species is it often seen in?
inflammation of serous membrane seen in dogs and cats caused by direct instillation such as trauma or fighting
38
What 3 "syndromes" are seen in nocardia spp.
pyothorax/peritonitis abscesses bovine mastitis
39
What is a distinguishing histological feature of nocardia?
beading or branching filaments
40
Who does pyothorax/peritonitis affect?
dogs and cats who are immunocompromised
41
What is bovine mastitis associated with? Is it curable?
intramammary infusions poor prognosis - cull
42
What syndrome does D. congolensis cause?
dermatophilosis - rain scald, greasy heel, lumpy wool, strawberry footrot
43
What species is affected by dermatophilosis?
mostly ruminants and horses
44
When does dermatophilosis commonly occur?
prolonged wet conditions causing skin maceration which allows organisms to invade epidermis
45
What is the basic lesion of dermatophilosis?
exudative dermatitis with thick scab formation
46
Explain the life cycle of dermatophilus
motile cocci (zoospores) elongate into rods, divide into multiple planes, motile cocci (zoospores)
47
What 2 main diseases do rhodococcus equi cause?
rattles cellulitis/lymphadentis
48
What is rattles?
pyogranulomatous bronchopneumonia of foals causing significant morbidity and mortality
49
What disease can be associated with type III hypersensitivity?
rattles
50
What is cellulitis/lymphadentis?
pyogranulomatous lesions that spread to lymph nodes in cats
51
What are 4 steps to diagnose infections?
1. signalment, history, clinical signs 2. obtain sample from lesions 3. perform a gram stain/diff quik 4. suspect either actinomyces of nocardia
52
What are 3 ways to treat infections?
1. surgical debridement, excision, or drainage 2. topical therapy 3. antibiotics
53
What are the drugs of choice for actinomyces and dermatophilus?
penicillin +/- streptomycin or tetracyclines
54
Penicillin +/- streptomycin are not the drugs of choice for nocardia or rhodococcus. Why?
They are intracellular - those antibiotics do not go into the cell
55
What is the drug of choice for rhodococcus?
macrolides AND rifampin
56
Why is susceptibility testing recommended for nocardia?
unpredictable