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Flashcards in Basic bacteriology Deck (25):
1

What components of the cell wall and outer membrane induce TNF and IL-1?

Lipoteichoic acid (cell wall is major surface antigen)

Lipid A (O polysaccharide)

2

Chocolate agar and factors

H. flu

Must include Factor V (NAD) and Factor X (hematin)

3

Thayer Martin agar

Neisseria

4

MacConkey agar

Lactose-fermenting enterics turn PINK

Enterobacter, E. Coli, Klebsiella

5

EMB agar

E. coli

Colonies form green metallic sheen

6

Charcoal yeast extract agar with cysteine and iron

Legionella

7

Obligate intracellular

Rickettsia, Chlamydia, Coxiella

Rely on host ATP

8

Encapsulated bacteria vaccines

Polysaccharide capsule is conjugated to a carrier protein (Diptheria toxin), promotes T cell activation and class switching

Pneumococcal, H. flu, Meningococcal

9

Catalase + organisms

Nocardia, Pseudomonas, Listeria, Aspergillus, Candida, E. coli, Staph, Serratia

10

Protein A

Binds Fc region of IgG

S. aureus

11

IgA protease

cleaves IgA

SHiN (Strep pneumo, H flu, Neisseria)

12

M protein

Prevent phagocytosis

Group A strep

13

Diptheria toxin

inactivate elongation-factor 2

14

Pseudomonas toxin

Exotoxin A

inactivate elongation-factor 2

15

Shigella toxin

Shiga

inactivate 60S ribosome by removing adenine from tRNA

causes HUS (enhances cytokine release)

16

EHEC toxin

Shiga-like toxin

inactivate 60S ribosome by removing adenine from tRNA

causes HUS (enhances cytokine release)

17

ETEC toxin

heat-labile = incr. cAMP --> incr. Cl secretion

heat-stable = incr. cGMP --> decr. resorption

18

Bacillus anthracis toxin

Edema toxin

increased cAMP

19

Vibrio cholerae toxin

Cholera toxin

overactivates adenylate cyclase (incr. cAMP), incr. Gs

incr. Cl secretion

20

Pertussis toxin

overactivates adenylate cyclase, disables Gi

impaired phagocytosis

21

C. perfringens toxin

alpha toxin

Phospholipase/lecithinase --> myonecrosis

22

Strep pyogenes

Streptolysin O

degrades cell membrane, lyses RBCs, contributes to B-hemolysis

23

Transformation

ability to take up naked DNA from environment

SHiN bacteria

prevent by adding deoxyribonuclease

24

Transposition

Segment of DNA moves from one location in the genome to another

Allows for DNA to move from plasmid to chromosome

25

Transduction

Phage moves DNA from one bacteria to another

Lysogenic phages contain DNA for 5 toxins

Shiga-like toxin
Botulinum
Cholera
Diphtheria
Strep pyogenes