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Flashcards in BB Ch27 - Genetic Monitoring Deck (24)
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1

2. The first (oldest) inbred mouse strain was ___ and was
described in ____ ?

DBA, 1909

2

3. What does “Genetically defined” mean?

Genetic homogeneity between individual animals of a breeding
group.

3

4. Which of the following fit the definition of
“genetically-defined”?
a. F1 hybrids (between inbred strains)
b. Outbred
c. Inbred and outbred
d. Random-bred, inbred

a

4

Many inbred strains were initially developed to study the role
of genes in ?

cancer

5

What is a common chemical agent used to generate mice carrying
point mutations?

ENU

6

At F20 of brother-sister matings, the number of loci that remained unfixed is estimated
to be?

273

7

What is one of the cardinal indicators of genetic problems in
a colony?

Improved reproductive performance

8

Congenic strains .
a. Do not need to be genetically monitored at each back cross
generation.
b. Are created by complete back crossing to the recipient strain
usually 10-12 generations.
c. Can be made faster if the animal with the least residual
homozygosity is chosen for the breeder in the subsequent
generation.
d. Are rare because to take over 3 years to produce them.

b

9

The majority of mutations remain silent, mutations that occur
in ____ and ______ , may alter physiology of a given strain.

Coding and regulatory regions

10

What is the most common source of genetic variation within
rodent colonies?________

human error

11

“Speed congenics” are made using _____ markers.

PCR amplified SSLP or SNPs

12

Tail skin grafting should be observed for _____ before drawing conclusions.

100 days

13

Name three immunologic assays used to detect genetic
differences among strains of rodents.

microcytotoxic assay, Hemagglutination assays, Mixed
lymphocyte reaction.

14

In the pyramid breeding system the top of the pyramid is?

foundation stock

15

Tail skin grafting is an easy way to check strain purity.

True

16

The mandible is the base of choice for identifying
osteometric traits.

True

17

Biochemical markers are a good tool for use in a genetic
quality-control program.

True

18

A minimum of ____ biochemical markers will provide a unique
profile for every independent inbred strain.

10

19

If separate lines are created at F20 with continued in breeding the number of loci differing between the two eventual
substrains is estimated to be ____.

117

20

More than ____ PCR reactions are required to generate a
single congenic generated by “speed congenics”.

3000

21

PCR based-assays for genetic monitoring may replace
immunologic assays in the future, 12 MHC haplotypes can be
identified by ___ PCR reactions..

2

22

The mutation rate of cryopreserved embryos is zero.

True

23

Who created the first inbred mouse strain?

Clarence Cook Little

24

Maintenance of an isogenic strain requires backcrossing to the parental inbred strain approximately every __ generations.

10