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Flashcards in Jax: Rules of Nomenclature of Mouse & Rat Strains Deck (34)
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1

True or False: Rules for strain nomenclature are not applicable to strains of both species.

False

2

Most of the laboratory mouse strains have contributions from

            a.         Mus musculus musculus

            b.         Mus musculus domesticus

            c.         Mus musculus molossinus

            d.         Mus musculus castaneus

            e.         All of the above

     e.         All of the above

3

The common laboratory rat strains derive from which species?

Rattus norvegicus.

4

What is the Laboratory Registration Code or Laboratory Code?

It is a code of usually three to four letters (first letter upper case, followed by all lower case) that identifies a particular institute, laboratory, or investigator that produced, and may hold stocks of, a mouse or rat strain.

5

Laboratory Codes are assigned by

            a.         National Research Council (NRC)

            b.         National Institutes of Health (NIH)

            c.         The Jackson Laboratory (JAX)

            d.         Institute of Laboratory Animal Research 

            e.         None of the above

        d.         Institute of Laboratory Animal Research 

6

When can a strain be named inbred?

If they have been mated brother x sister for 20 or more consecutive generations, and individuals of the strain can be traced to a single ancestral pair at the 20th or subsequent generation.

7

True or False: Inbred strains must be continuously mated brother x sister.

True.

8

Consecutive parent x offspring mating may be used for inbred strains provided that

a. the offspring that is mated to parent is never mated to its offspring

b. the parent is mated to all its subsequently offspring

c.  the offspring that is mated to parent is subsequently mated to its offspring

d. all of the above

e. none of the above

c.  the offspring that is mated to parent is subsequently mated to its offspring

9

What of the followings is an inbred strain?
a. CD1
b. Swiss
c. Wistar
d. 129P1
e. D2B6F1

d. 129P1

10

True or False: An inbred strain should be designated by a unique brief symbol made up of upper case roman letters or a combination of letters and numbers beginning with a letter.

True

11

What is the symbol used to indicate the generations of inbreeding?

F - Filial

12

A substrain will result if
a. branches are separated after 20 but before 40 generations of inbreeding
b. branches are separated for more than 20 generations from a common ancestor
c. genetic differences are proven by genetic analysis to have occurred between branches
d. all of the above
e. none of the above

d. all of the above

13

Which of the following are not substrains?
a. FL/1, FL/2
b. DBA/1, DBA/2
c. BALB/cHeAn, BALB/cJ
d. C57BL/6, C57BL/10
e. None of the above

b. DBA/1, DBA/2

14

True or False: Organizations distributing mice and rats should include the number of generations the strain has been separated from the parent strain in the information they provide regarding the strain.

True.

15

How are hybrids designated?

Hybrids can be designated using upper case abbreviations of the two parents (maternal strain listed first), followed by F1.

16

True or False: Reciprocal F1 hybrids are genetically identical.

False.

17

What is the abbreviation for each of the following...
a. BALB/c
b. C57BL/6
c. C3H
d. CBA
e. DBA/2

a. C
b. B6
c. C3
d. CB
e. D2

18

Recombinant inbred strains have equal proportions of genetic contributions from two original progenitor inbred strain.(T/F)

True

19

How many crossings of inbred strains needed to create a recombinant inbred strain?
a. 10
b. 5
c. 20
d. 30

c. 20

20

CXB  (cross of BALB/c x C57BL/6J) is...
a. Mixed inbred strain
b. Recombinant inbred strain
c. Hybrid strain
d. Recombinant Congenic Strain

b. Recombinant inbred strain

21

Which statement is FALSE about mixed strains?
a. Could be mutant strain, incipient or inbred
b. Derived from more than two progenitor strains
c. Having genetic contribution from a known sources
d. May be designated as STOCK Rb(16.17)5Bnr

c. should have been unknown sources

22

Incipient inbred strains separated by which of these symbols?
a. Colon            :
b. semicolon     ;
c. Slash             /
d. Period           .

b. semicolon     ;

23

Recombinant Congenic (RC) Strains are formed crossing two inbred strains, followed by a few (usually two) backcrosses of the hybrids to one of the parental strains (the "recipient" strain), with subsequent inbreeding without selection for any specific marker.  (T/F)

True

24

Recombinant congenic strain between BALB/c recipient and STS donor are separated by
a. Letter “c”    CcS
b. Letter “R”   CRS
c. Letter “C”   CCS
d. Letter “r”     CrS

a. Letter “c”    CcS

25

What is Pri:B6,D2-G#
a. Advanced intercross lines
b. Recombinant congenic strain
c. Recombinant inbred
d. Transgenic strain

a. Advanced intercross lines

26

In development of Recombinant Congenic , one generation of backcrossing will be regarded as being equivalent to two generations of brother x sister mating. (T/F)

True

27

With regard to coisogenic strains, which statement is FALSE?
a. are inbred strains that differ at only a single locus
b. chemically or radiation induced mutants on an inbred background
c. A coisogenic strain may accumulate genetic differences over time by genetic drift
d. are inbred strains that differ by a single chromosome

d. are inbred strains that differ by a single chromosome

28

How many backcross are needed to produce congenic strain?
a. 5
b. 10
c. 15
d. 20

b. 10

29

Speed congenic (marker assisted breeding) requires how many generations?
a. 10
b. 5
c. 15
d. 3

b. 5

30

Regarding congenic strains, congenic donor strain contribution unlinked to the selected locus or chromosomal region is less than
a. 0.01
b. 0.001
c. 0.1
d. 1

a. 0.01

31

Regarding consomic strains, which statement is FALSE?
a. Recipient strain acquired whole chromosome by repeated backcrossing
b. Recipient acquired strain unique allele by repeated backcrossing
c. a minimum of 10 backcross generations is required
d. Example: “C56BL/6J-Chr 19SPR”

b. Recipient acquired strain unique allele by repeated backcrossing

32

Regarding segregating inbred strains , which statement is FALSE?
a. particular allele or mutation is maintained in heterozygous state
b. particular allele or mutation is maintained in homozygous state
c. They are developed by inbreeding (usually brother x sister mating)
d. Designated by B6.Cg-m +/+ Leprdb

b. particular allele or mutation is maintained in homozygous state

33

Regarding conplastic strains, which statement is FALSE?
a. nuclear genome from one strain has been crossed onto the cytoplasm of another
b. Example:  57BL/6J-mt BALB/c
c. the mitochondrial donor is always the female parent
d. the mitochondrial donor is always the male parent
e. a minimum of 10 backcross generations is required

d. the mitochondrial donor is always the male parent

34

Congenic lines that differ at a histocompatibility locus and therefore resist each other's grafts are called congenic resistant (CR) lines.  (T/F)

True