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Flashcards in SBG Mouse Qs_BBCh3 Deck (35)
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1

What is the full taxonomic classification of the house mouse?

kingdom Animalia, phylum Vertebrata, class Mammalia, order Rodentia, family Muridae, subfamily Murinae, Mus musculus

2

2. What is diploid chromosome number of mice?

40

3

3. Specific genes can be visualized on chromosomes by what cytogenetic technique?

FISH - fluorescent in situ hybridization

4

4. Which genes are responsible for rejection of foreign skin grafts within 10-20 days, and where are these genes located in the mouse?

genes in the major histocompatibility complex (MHC, H2) located in chromosome 17

5

5. Which genes are responsible for delayed graft rejection and where are they located in the mouse?

minor histocompatibility (H) genes located throughout the genome

6

6. Other than graft rejection, what are the other functions of the H2 complex?

Cell-cell interactions in primary immune responses and the level of response to a given antigen
Immune-mediated responses to infectious agents such as viruses and complement activity

7

7. What is the definition of an inbred strain of mice?

brother-sister matings for more than 20 generations, but should not be considered completely inbred until after 40 generations of sib matings

8

What is the primary research use of wild-derived inbred strains of Mus musculus castaneus and Mus spretus?

genetic mapping because of large numbers of polymorphic differences from standard inbred laboratory mice

9

9. What is the definition of a coisogenic inbred strain?

a mutation of interest occurred within that strain

10

How can a coisogenic strain be perpetuated?

brother-sister matings within strain of origin
Backcross or cross-intercross system within strain of origin
Brother-sister mating with heterozygosity forced by back- or intercrosses, or
Brother-sister mating between homozygotes

11

11. What is a congenic?

when the mutation of interest was transferred from another stock or strain by repeated backcrossings

12

How can a congenic strain be perpetuated?

brother-sister matings within strain of origin after 10-12 generations of backcrossing with periodic backcrosses to background strain
Backcross or cross-intercross system within strain of origin
Brother-sister mating with heterozygosity forced by back- or intercrosses, or
Brother-sister mating between homozygotes

13

In a random bred stock population of 25 breeding pairs, heterozygosity will ___________ at ________ per generation with standard randomization techniques.

decrease, 1%

14

14. A common random breeding program called circular pair mating system involves each pair mating how many times?

once

15

15. What is a recombinant inbred strain?

sets of inbred strains developed by single-pair random matings of mice from an F2 generation created by crossing mice of two inbred strains

16

16. How are recombinant inbred strains perpetuated?

brother-sister matings for more than 20 generations to obtain homozygosity; can take up to 7 years to produce

17

What is the main general research purpose of recombinant inbred strains?

mapping phenotypic or quantitative traits that differ between progenitor strains

18

18. What is a recombinant congenic strain?

Sets of inbred strains derived in a manner similar to RI strains except that one or more backcrosses to one parental strain (background strain) are made after the F1 generation, before inbreeding has begun. The other parental strain is designated as the donor strain. The proportion of background and donor genomes is determined by the number of backcrosses preceding inbreeding.

19

What is an advanced intercross line (AIL)?

made by producing an F2 generation between two inbred strains and then, in each subsequent generation, intercrossing mice but avoiding sibling matings. The purpose is to increase the possibility of recombination between tightly linked genes.

20

Describe: DBA/2J

inbred strain; named for coat color genes dilute (d), brown (b), and nonagouti (a); second of two sublines separated before 20 generations of sib mating and is the subline maintained at Jackson Laboratories (J)

21

21. Describe: Hsd:ICR

ICR outbred stock maintained at Harland

22

22. Describe: C3H/HeSn-ash/+

coisogenic segregating inbred mutant strain carrying the ashen (ash) mutation, which arose on C3H/ HeSn

23

23. Describe: AKR.B6-H2b

congenic inbred strain in which the b haplotype at the H2 complex was transferred from C57BL/6J (B6) to the AKR background

24

24. Describe: BXD-1/Ty

recombinant inbred (RI) strain number 1 in a set of RI strains derived from a C57BL/6J (B) female mated to a DBA/2J (D) male and made by Taylor (Ty)

25

25. Describe: CcS1

recombinant congenic (RC) strain number 1 in a set made by crossing the BALB/c (C) and STS (S) strains, backcrossing 1 or 2 times to BALB/c and then inbreeding as with RI strains

26

26. Describe: CcS1(N4)

recombinant congenic (RC) strain number 1 in a set made by crossing the BALB/c (C) and STS (S) strains, backcrossing N4 times to BALB/c and then inbreeding as with RI strains

27

27. How much feed do mice typically consume per day after weaning?

3-5 g/day

28

28. How much water do adult mice typically consume per day?

6-7 ml/day

29

29. How does a mouse respond to cold exposure?

non-shivering thermogenesis (activated by norepinephrine)

30

30. What is the biological half-time of water for mouse?

1.1 days, more rapid than for larger mammals

31

T/F Mice have no sweat glands

True

32

32. What is the thermoneutral zone for mice?

approximately 29.6-30.5 C, narrower than that of any other mammal measured thus far

33

T/F Mice reared in the thermoneutral zone grow faster, have larger litters, and more viable pups than those reared outside that range.

F, optimal range for having larger litters, growing faster, and more viable pups is 21-25 C. Thermoneutrality should not be equated with comfort or physiological economy.

34

34. What is oxygen consumption of a typical mouse at rest?

3.5 ml O2/g/hr

35

35. What physiologic and anatomical mechanisms allows the mouse to accommodate having such a high metabolic rate?

high alveolar PO2
Rapid respiratory rate
Short air passage
moderately high RBC
high RBC hemoglobin and carbonic anhydrase concentrations
High O2 blood capacity
Slight shift in O2-dissociation curve enabling O2 to be unloaded in the tissue capillaries at high PO2
More pronounced Bohr effect (hemoglobin affinity for O2 with changes in pH is more pronounced)
High capillary density
High blood glucose concentrations