Name the order, family, subfamily, and genus of the lab mouse.
Order Rodentia, family Muridae, subfamily Murinae, genus Mus.
Mice have ___ chromosomes.
The ______loci control expression of cell surface molecules that modulate major immunological phenomena. This complex is one of the most studied genetic systems of the mouse.
Mating mice of two inbred strains produces what?
F1 hybrid mice
Recombinant inbred strains a developed how?
By single-pair random matings of mice from an F2 generation created by crossing mice of two inbred strains.
How are transgenic strains named?
Symbol for the strain followed by a symbol for the transgene- e.g. Tg(YYYYY)#Zzz, where Tg = transgene, (YYYYY) = description of the inserted DNA, # = assigned number in the series of events generated using a given construct, Zzz = lab code
What are some criteria for rodent contact bedding?
Moisture absorbent, dust free, does not promote microbial growth, atraumatic, sterilizable, uniform batch to batch, nontoxic, inexpensive
Adult mice drink ____ml of water per day.
Due to the large surface area per gram of body weight, mice have significant physiologic changes in response to change in ambient temperature. T or F
The neonatal mouse is ectothermic. T or F
The neonatal mouse develops temperature control about ____ days of age.
20 days of age
The thermoneutral zone (29.6-30.5 C) is the optimum temperature for mice to live. T or F
False- 21-25 C is the idea temperature for mice, not the thermoneutral zone
The right lung has ____lobes; the left lung has _____.
Right = 4 lobes; left = 1 lobe
Describe the stomach of the mouse.
The proximal portion is keratinized, the distal portion is glandular.
The altered Schaedler flora (ASF) are given to mice to make them ___________ (know microbiota).
What bacteria are in the "altered Schaedler flora" (ASF)?
Four members of the original Schaedler flora (two lactobacilli, Bacteroides distasonis, and the EOS fusiform bacterium), a spiral-shaped bacterium, and three new fusiform EOS bacteria.
The thymus reaches maximum size at ________ and involutes at _________.
sexual maturity, 35-80 days of age.
Mean systolic blood pressure is (range)__________ and heart rate is (range)_______.
84-105 mm Hg, 310-840/ min
Normal dentition in a mouse is ____ incisor and ____ molars in each quadrant.
The female mouse has how many pair of mammary glands?
Sexual maturity occurs in the female at _____ days and the male at ____.
24-28 days, up to 2 weeks later
Describe the estrous cycle of the mouse.
Polyestrous, cycles every 4-5 days, spontaneous ovulators
Ovulation does not accompany every estrus, and estrus may not coincide with every ovulation. T or F
What is the Whitten effect?
Estrus is suppressed in mice housed in large groups because of pseudopregnancy or diestrus
What is the Bruce effect?
Pheromones from a strange male mouse may prevent implantation or pseudopregnancy in recently bred females.
Gestation is ___ days.
There are two categories of pheromones. Name them.
Primer and releaser pheromones
There are ____ classes of immunoglobulins.. They are :
5 , IgM, IgD, IgG (subtypes IgG1, IgG2a, IgG2b, IgG3, IgA, IgE
Mouse T lymphocytes can be differentiated into 2 phenotypes:
CD4+ T cells (MHC class II) and CD8+ T cells (MHC class I)
Definition of Inbred
Strain established by a minimum of 20 consecutivegenerations of sister × brother matings (sib matings)
Definition of Outbred
Stock/line established and maintained by strictavoidance of sib mating
Created by mating mice of two inbred strains in adefined direction
Created by mating two F1 hybrid mice
Lines established by inbreeding of the progeny ofindividual F2 hybrid sibling pairs for at least 20generations
Introduction of a mutation onto a defined inbredstrain background by 5–10 or more generations ofmatings back to the inbred strain with selection forthe mutation (backcrossing)
An inbred strain that carries one chromosome fromanother inbred strainTen generations of backcrossing and selection for thedonor chromosome is required for a fully consomicstrain
An inbred strain that carries the nuclear genome ofone inbred strain and the cytoplasm and cytoplasmic(mitochondrial) genome of anotherSince the egg contributes the embryonic cytoplasm,10 generations of backcrossing inbred females to aninbred male recipient is required for a fully conplasticstrain
Restriction fragment length polymorphism - DNA is isolated, digested by bacterial restriction enzymes, then the lengths of the digested fragments compared for differences.
Single Nucleotide Polymorphisms - identified variations in single nucleotides used to monitor genotypes