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Microbiology > Ch 03 Microscope > Flashcards

Ch 03 Microscope Flashcards

1
Q

How is Robert Hooke’s invention related to Joseph Lister’s invention?

A

The compound lens and microscope

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2
Q

Was was van Leeuwenhoek’s invention?

A

Single lens 300X microscope

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3
Q

What is the advantage to oil immersion lenses?

A

Oil immersion lenses corrects the refractive index of glass which is a problem at high magnification.

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4
Q

Which lens is the occular and which is the objective?

A

Ocular: lens nearest the eye
Objective: lens nearest the slide

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5
Q

What is a smear?

A

A thin film of material containing the microbe to be studied under a microscope

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6
Q

What is a mordant?

A

A chemical added to the smear to intensify the stain

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7
Q

What devices focus light and electron microscopes?

A

Light - lenses

Electron - magnets

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8
Q

What is a chromophore?

A

Part of the molecule that reflects wavelengths back to the eye

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9
Q

What is Carolus Linnaeus contribution to science?

A

Developed two-part naming system

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10
Q

What is Robert Whitaker’s contribution to science?

A

Founded the five kindom system in which prokaryotes were placed in the prokaryote kindom

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11
Q

What is serology?

A

The study of blood serum and other bodily fluids

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12
Q

Which microscope is used to look at spirochetes?

A

Darkfield

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13
Q

What is the maximum resolving power and magnification of light and electron microscopes?

A

Light: 0.2 um, 2,000X
2D EM: 10 pm, 100,000X
3D EM: 10 nm, 10,000X

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14
Q

What is a simple stain?

A

Water or alcohol and dye

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15
Q

What can be used to improve the bonding between a simple stain and the specimen?

A

A modrant

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16
Q

In Two-Photon microscopy, what kind of dye is used?

A

Fluorescent dye

17
Q

In Scanning Acoustic Microscopy, what is the medium used?

A

Sound waves

18
Q

What kind of images do Scanning-tunneling Microscopy and Atomic Force Microscopy yeild?

A

3D

19
Q

Name two differential stains

A

Gram stain: violet, iodine, alcohol decolorizer, red

Acid-Fast stain: acid-fast microbes for red,
non-acid fast methylene blue counterstain

20
Q

What stain is used to make capsules visible?

A

Special stains

21
Q

What stain is used to make endospore visible?

A

Special stains

22
Q

A cell is stained because of __________?

A

ionic interaction

Microbiology (23 decks)

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