Ch 03 Microscope Flashcards Preview

Microbiology > Ch 03 Microscope > Flashcards

Flashcards in Ch 03 Microscope Deck (22):
1

How is Robert Hooke's invention related to Joseph Lister's invention?

The compound lens and microscope

2

Was was van Leeuwenhoek's invention?

Single lens 300X microscope

3

What is the advantage to oil immersion lenses?

Oil immersion lenses corrects the refractive index of glass which is a problem at high magnification.

4

Which lens is the occular and which is the objective?

Ocular: lens nearest the eye
Objective: lens nearest the slide

5

What is a smear?

A thin film of material containing the microbe to be studied under a microscope

6

What is a mordant?

A chemical added to the smear to intensify the stain

7

What devices focus light and electron microscopes?

Light - lenses
Electron - magnets

8

What is a chromophore?

Part of the molecule that reflects wavelengths back to the eye

9

What is Carolus Linnaeus contribution to science?

Developed two-part naming system

10

What is Robert Whitaker's contribution to science?

Founded the five kindom system in which prokaryotes were placed in the prokaryote kindom

11

What is serology?

The study of blood serum and other bodily fluids

12

Which microscope is used to look at spirochetes?

Darkfield

13

What is the maximum resolving power and magnification of light and electron microscopes?

Light: 0.2 um, 2,000X
2D EM: 10 pm, 100,000X
3D EM: 10 nm, 10,000X

14

What is a simple stain?

Water or alcohol and dye

15

What can be used to improve the bonding between a simple stain and the specimen?

A modrant

16

In Two-Photon microscopy, what kind of dye is used?

Fluorescent dye

17

In Scanning Acoustic Microscopy, what is the medium used?

Sound waves

18

What kind of images do Scanning-tunneling Microscopy and Atomic Force Microscopy yeild?

3D

19

Name two differential stains

Gram stain: violet, iodine, alcohol decolorizer, red


Acid-Fast stain: acid-fast microbes for red,
non-acid fast methylene blue counterstain

20

What stain is used to make capsules visible?

Special stains

21

What stain is used to make endospore visible?

Special stains

22

A cell is stained because of __________?

ionic interaction