Chapter 7 Flashcards
Absence of significant contamination
Asepsis
Refers to microbial contamination. Condition resulting from growth and spread of bacteria in blood in tissues
Sepsis
Removal of pathogens from living tissue
Antisepsis
Removal of all microbial life including endospores
Sterilization
Process of treatment especially of canned foods to kill the vegetative cell and kill Clostridium botulinum endospores
Commercial sterilization
Removal of pathogens from non-living surfaces
Disinfection
Removal of microbes from a limited area, the skin is swabbed with alcohol for injection
Degerming
Reducing microbial counts on eating utensils and from good preparation areas
Sanitization
Substance that kills microbes
Biocides/Germicide
Inhibiting
Not killing
Bacteria
Bacteriostasis
Number of microbes
Environment
Time of exposure
Microbial characteristics
Effectiveness of microbial treatments
Alteration of membrane permeability
Damage to proteins
Damage to nucleic acids
Actions of microbial control agents
Kills microbes by denaturing enzymes
Moist heat
Lowest temperature at which all cells in a culture are killed in 10 min.
- Heat resistance of bacteria
- Clumping of the cells
- Amount of water present
- Presence of organic matter
- Past history of treatment
Thermal death point
Minimum length of time to kill all bacteria in a liquid culture at a given temperature
Thermal death time
Minutes to kill 90% of a population at a given temperature
Boiling kills many vegetative cells and viruses within 10 mins
Decimal reduction time
Stem under pressure
Is most effectively method of moist heat sterilization
Kills microbes
Auto calving
Reduces spoilage organisms and pathogens from foods. Kills vegetative cells
Pasteurization
63 degrees for 30 mins
Low temp-long time
72 degrees for 15 seconds
High temp-short time
140 degrees for 1 seconds
Ultra high temperature
Heat resistant organisms survive pasteurization but do not cause any threat because they will grow at storage temp
Thermoduric
Kills by oxidation
Flaming inculcating loops
Incineration
Hot air sterilization.
Dry heat sterilization
Filtration is the passage of a liquid or gas through a filter with pores small enough to retain microbes
Used to sterilize heat-labor heat sensitive materials like enzymes, vaccines, antibiotics
Microbes can be removed from air by high-efficiency particulate air filters
Filtration
