Chapter 18 Flashcards
(21 cards)
Suspension of living organisms or fractions of organisms or inactivated bacterial toxins that are used to induce active immunity
Vaccination
Inoculation if smallpox into skin with a needle
Variolaton
Use living but weakened microbs (MMR)
Attenuated vaccines
Hep-B
Use antigenic fragments if produced through genetic engineering
Sub unit vaccines
Sensitivity of a diagnostic test is determined by the percentage of positive samples it correctly detects and free specificity is determined by the percentage of false positives it gives
Diagnostic immunity
Use antibodies linked to an enzyme
Antigen-antibody reactions are detected by enzyme activity. If indicator enzyme is present in test well, an antigen antibody reaction has occurred
ELISA
Used to detect antigens against a specific antibody bound to a test well
Direct ELISA
Used to detect antibodies against an antigen bound in a test well
Indirect ELISA
Technique can identify microorganisms in a clinical sample and detect the presence of a specific antibody in serum
Fluorescent antibody techniques
Used to identify specific microorganisms
Direct FA
Are used to demonstrate the presence of antibodies in serum
Indirect FA
Serological test based on the depletion of fixed ambling of complement In the presence of an antigen-antibody reaction
1. Complement fixation.
2. Indicator stage
Positive reaction no hemolysis
Complement Fixation.
Eliminate the harmful Effect of a virus or exotoxin by a specific antibody
Neutralization
Antibody produced in response to a bacterial exotoxin or a toxoid that neutralizes the exotoxin
Antitoxin
Antibodies against a virus can be detected by antibodys ability to prevent cytopathic effects of viruses in cell culture
Virus neutralization test
Reaction using RBC
used in blood typing the diagnosis of certain disease and identification of viruses
Hem agglutination
Involves the clumping together of insoluble molecules
Involve particular antigens and antibodies
Agglutination
On a cell
Direct agglutination
Attached to latex spheres
Indirect agglutination
Involve interaction of soluble antigens with IgG or IgM antibodies
Formation if precipitation reactions lattices
Precipitation reactions
Studies serum and immune response that are evident in the serum
Direct test: detect antigens from patient sample
Indirect test: detect antibodies in patient serum
Serological test