Chapter 9 Flashcards Preview

Microbiology > Chapter 9 > Flashcards

Flashcards in Chapter 9 Deck (15):
0

Recombinant DNA is a DNA. Molecule produced by recombination that occurs naturally through transformation, conjugation, and transduction

Biotechnology & Recombinant DNA

1

Special class of DNA cutting enzymes that exist in many bacteria
Destroy bacteriophage DNA in bacteria cells that is why bacteriophages infect selected bacteria that do not produce specific restriction enzyme to kill the bacteriophages

Restriction enzymes

2

Carry DNA to desired cell
Plasmid and viruses
Self replicating
Circular DNA
R factor plasmids preferred
Restriction enzymes are used to insert desired DNA into the vector

Vectors

3

Can exist in several different species and are used to move cloned sequence of DNA among different organism like Bactria, fungi, plants, mammals, yeast

Vectors

4

DNA in viral cloned inside the host

Vectors

5

Retroviruses
Adenovirus
Herpesviruses

Human gene therapy

6

To make multiple copies of a piece of DNA enzymatically
Clone DNA for recombination
Amplify DNA to detectable levels
Diagnose genetic disease
2 cycles
Sequence DNA

PCR

7

Transformation
Electroproration
Protoplast
Micro injection
Gene gun

DNA insertion

8

Procedure in which cell can pick up DNA from surrounding environment
E. Coli yeast, mammalian are not competent

Transformation

9

Process uses electrical current to form microscope pores in the membrane of the cell through the pores
Applicable to all cells
Protoplasm

Electroporation

10

Protoplast fuse at slow rate In solution of polyethylene glycerol
DNA of cells may undergo natural recombination
Plant algal cells may be genetically manipulated this way

Protoplast fusion

11

Direct way of inserting DNA into an animal cell

Microinjection

12

Can also be used to insert DNA coated bullets of tungsten or hold I to a cell with the help of burst if helium

Gene gun

13

Made from mRNA by reverse transcriptase
mRNA no introns

cRNA

14

Process in which DNA is made from mRNA by reverse transcriptase.

Reverse transcriptase