Chemistry 4 (Proteins)

Question 1

recommended method for total protein

Answer 1

Biuret technique
copper salts turn purple colon
absorbance at 540nm

Question 2

proteins absorb UV light at

Answer 2

210 and 280 nm

Question 3

T1/2 albumin

Answer 3

17d

Question 4

fastest migrating protein on SPEP

Answer 4

prealbumin (transthyretin)

Question 5

2 functions of prealbumin

Answer 5

binds T3 and T4

complexes with Vitamin A

Question 6

prealbumin is the amyloid precursor in

Answer 6

senile cardiac amyloidosis

Question 7

T1/2 of prealbumin is

Answer 7

48hr

Question 8

true increase in prealbumin seen in

Answer 8

chronic alcoholic

corticosteroid use

Question 9

predominant alpha 1 band

Answer 9

alpha1-antitrypsin

Question 10

minor component of alpha band but highly increased in acute inflammation

Answer 10

alpha1-acid glycoprotein

Question 11

large protease inhibitor which is not lost in nephrotic syndrome

Answer 11

alpha2-macroglobulin

Question 12

3 components of alpha 2 band

Answer 12

alpha2-macroglobulin
ceruloplasmin
haptoglobin

Question 13

3 components of beta band

Answer 13

transferrin
fibrinogen
complement (C3)

Question 14

transferrin increased in

Answer 14

iron deficiency
pregnancy
estrogen

Question 15

transferrin in CSF

Answer 15

double peak from asialated transferrin (Tau)

Question 16

Carbohydrate deficient transferrin is a marker for

Answer 16

EtOH use

Question 17

fibrinogen may be seen in serum in

Answer 17

heparin treatmtnte, dysfibrinogenemia, APL, liver dz, Vit K def

Question 18

protein besides Ig in gamma region

Answer 18

CRP

Question 19

dz assd with selective loss of albumin

Answer 19

minimal change

Question 20

band that is not decreased in nephrotic syndrome

Answer 20

alpha2 (alpha2 macroglobulin)

Question 21

SPEP in acute inflammation

Answer 21

increased alpha 1 and alpha2
decreased albumin
eventual polyclonal increase in Ig

Question 22

beta-gamma bridging seen in

Answer 22

cirrhosis (due to increased IgA)

Question 23

monoclonal protein seen in

Answer 23

myeloma, waldenstrom (LPL), CLL/SLL

Question 24

biclona peak can be see in what gammopathy

Answer 24

IgA due to monomers and dimers

Question 25

____ assay very sensitive to myeloma recurrence

Answer 25

free light chain assay

Question 26

Hyperviscosity syndrome when viscosity >___

Answer 26

3cp

Question 27

measure viscosity when Ig___

Answer 27

IgM>4 | IgG or A >6

Question 28

Characteristics of CSF electrophoresis

Answer 28

increased prealbumin double B (transferrin) dimmer albumin

Question 29

Characteristics of glomerular proteinuria

Answer 29

very increased albumin increast AAT increased transferrin

Question 30

characteristics of tubular proteinuria

Answer 30

increased alpha 2, beta, and light chains

Question 31

procedure to detect cryoglobulins

Answer 31

draw, clot, and centrifuge at 37 | store at 4 for 3d then centrifuge at 4

Question 32

type 1 cryoglobulinemia

Answer 32

monoclonal (MM or waldenstrom)

Question 33

Type 2 cryoglobulinemia

Answer 33

``` monoclonal IgM (rheumatoid factor) with polyclonal IgG ```

Question 34

type 3 cryoglobulinemia

Answer 34

2 polyclonal Ig's

Question 35

most common cuase of mixed cryoglob

Answer 35

HCV

Question 36

sx of cryoglob

Answer 36

leukocytoclastic vasculitis (palpable purpura) arthralgias, organomegaly lymphadenopathy, anemia sensorineural defects, glomerulonephritis

Question 37

other lab abnormality in cryoglobulinemia

Answer 37

hypocomplementemia

Question 38

most common renal finding in cryoglob

Answer 38

MPGN type II (dense deposit dz)