EC Paper Flashcards Preview

Biochemistry 431 > EC Paper > Flashcards

Flashcards in EC Paper Deck (6)
1

Obg

is a universally conserved P-loop GTPase that acts at the crossroads of the major cellular processes of translation and DNA replication, processes thought to be repressed in dormant persister cells; y, it plays a role in DNA replication initiation, replication fork stabilization, and ribosome maturation, processes
that are targeted by many antibiotics

2

Deletion of RelA and SpoT

results in a dramatic decrease in HipA-mediated persistence.

3

high cellular levels of (p)ppGpp

were recently demonstrated to activate TA modules through a regulatory cascade depending on inorganic
polyphosphate and the Lon protease

4

global control of persistence in E. coli through

Obg

5

Obg-mediated persistence requires

(p)ppGpp and proceeds via transcriptional activation of the
type I hokB-sokB TA module, ultimately resulting in a decrease
in membrane potential.

6

antisense RNA (asRNA)-based approach

Used this approach because knockout obg could not be easily obtained; depleting Obg levels by inhibiting translation of obg mRNA; . Expression of obgasRNA caused a moderate 7-fold decrease in Obg concentration

Decks in Biochemistry 431 Class (80):